Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   113614
Name   oriT_S16BD04351|p106 in_silico
Organism   Salmonella enterica strain S16BD04351
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP099765 (86511..86634 [-], 124 nt)
oriT length   124 nt
IRs (inverted repeats)      92..99, 113..120  (ATAATGTA..TACATTAT)
 90..95, 107..112  (AAATAA..TTATTT)
 39..46, 49..56  (GCAAAAAC..GTTTTTGC)
 3..10, 15..22  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 124 nt

>oriT_S16BD04351|p106
GGTTGGTGGTTCTCACCACCAAAAGCACCACACACTACGCAAAAACAAGTTTTTGCTGATTTGCTACTTGAATCATTAACTTATGTTTTAAATAATGTATTTTAATTTATTTTACATTATAAAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   8723 GenBank   WP_180566001
Name   traI_NHV94_RS00360_S16BD04351|p106 insolico UniProt ID   _
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 191691.14 Da        Isoelectric Point: 5.5476

>WP_180566001.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQDPQLHTHVVVANVTQHNDEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAEWMQT
LKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELAMMAREQGREVQIIAADRRSQMNLKQDEGLSGELITGR
RQLQEGMVFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLSLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPASLPVSDSPFTALKL
ENGWVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVLESKNQAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRLEGTWAPEHSVTEFSHSQEAELAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWEKNPDALALVDSVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKPDREVMNAQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDCNGRSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDTQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAGQERDRADITEREAALPESVLRESQREQEAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

(633-712)

(968-1156)

(10-283)

(1434-1557)

(575-626)


  Protein structure



No available structure.




Auxiliary protein


ID   5108 GenBank   WP_001254388
Name   WP_001254388_S16BD04351|p106 insolico UniProt ID   W9A0Z2
Length   75 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 75 a.a.        Molecular weight: 9072.20 Da        Isoelectric Point: 10.2071

>WP_001254388.1 MULTISPECIES: conjugal transfer relaxosome protein TraY [Enterobacterales]
MRRRNARGGISRTVSVYLDEDTNNRLIRAKDRSGRSKTIEVQIRLRDHLKRFPDFYNEEIFREVIEENES
TFKEL

  Protein domains


Predicted by InterproScan.

(14-61)


  Protein structure


Source ID Structure
AlphaFold DB W9A0Z2

ID   5109 GenBank   WP_069067309
Name   WP_069067309_S16BD04351|p106 insolico UniProt ID   _
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14503.49 Da        Isoelectric Point: 4.8498

>WP_069067309.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Enterobacteriaceae]
MAKVQAYVSDEIVYKINKIVERRRAEGAKNTDVSFSSISTMLLELGLRVYETQMERKESAFNQTEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSSEMERFFPENDEG

  Protein domains


Predicted by InterproScan.

(1-126)


  Protein structure



No available structure.




T4CP


ID   10099 GenBank   WP_180566002
Name   traD_NHV94_RS00365_S16BD04351|p106 insolico UniProt ID   _
Length   726 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 726 a.a.        Molecular weight: 82565.27 Da        Isoelectric Point: 5.3214

>WP_180566002.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLILWVKISWQTFVNGCIYWWCTTLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYAR
KRGDMVVIYDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTYLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGESFTIRDWMRGVREDKKNGWLFISSNADTHASLKPVVSMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEYLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEGVTQAEQPQQPQQPQQPQQ
PQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQQENHPDIQQ
HMQRREEVNINVHRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

(32-128)

(173-560)

  Protein structure



No available structure.



ID   10100 GenBank   WP_069067322
Name   traC_NHV94_RS00460_S16BD04351|p106 insolico UniProt ID   _
Length   876 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 876 a.a.        Molecular weight: 99447.38 Da        Isoelectric Point: 6.8589

>WP_069067322.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacteriaceae]
MSKNPLETVTQAVNSLLTALKLPDESSQANQILGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAI
PINGANKSIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAA
ATQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIV
GEMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNPEIAF
LWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWG
ELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGK
GLFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSG
AGKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLS
VMASPNGNLDEVHEGLLLQAVKASWLAKKNQARIDDVVNFLKNASDSEQYAGSPTIRSRLDEMIVLLDQY
TANGTYGRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRTLKKLNVIDEGW
RLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKY
NQLFPDQFQPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREG
MSIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

(468-772)

(291-447)

(39-277)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 58396..87206

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NHV94_RS00365 58396..60576 - 2181 WP_180566002 type IV conjugative transfer system coupling protein TraD virb4
NHV94_RS00370 60573..61352 - 780 WP_069067317 hypothetical protein -
NHV94_RS00375 61555..62286 - 732 WP_000850429 conjugal transfer complement resistance protein TraT -
NHV94_RS00380 62317..62820 - 504 WP_000605509 hypothetical protein -
NHV94_RS00385 62835..65657 - 2823 WP_069067318 conjugal transfer mating-pair stabilization protein TraG traG
NHV94_RS00390 65654..67027 - 1374 WP_001137364 conjugal transfer pilus assembly protein TraH traH
NHV94_RS00395 67014..67406 - 393 WP_001744736 F-type conjugal transfer protein TrbF -
NHV94_RS00400 67387..67728 - 342 WP_001448115 P-type conjugative transfer protein TrbJ -
NHV94_RS00405 67658..68203 - 546 WP_000059832 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
NHV94_RS00410 68190..68474 - 285 WP_000623730 type-F conjugative transfer system pilin chaperone TraQ -
NHV94_RS00415 68601..68939 - 339 WP_001287905 conjugal transfer protein TrbA -
NHV94_RS00420 68955..69698 - 744 WP_001030371 type-F conjugative transfer system pilin assembly protein TraF traF
NHV94_RS00425 69691..69948 - 258 WP_000864329 conjugal transfer protein TrbE -
NHV94_RS00430 69975..71825 - 1851 WP_000821864 type-F conjugative transfer system mating-pair stabilization protein TraN traN
NHV94_RS00435 71822..72460 - 639 WP_001080257 type-F conjugative transfer system pilin assembly protein TrbC trbC
NHV94_RS00440 72469..72774 - 306 WP_069067320 hypothetical protein -
NHV94_RS00445 72804..73796 - 993 WP_069067321 conjugal transfer pilus assembly protein TraU traU
NHV94_RS00450 73793..74425 - 633 WP_001203727 type-F conjugative transfer system protein TraW traW
NHV94_RS00455 74422..74808 - 387 WP_000214082 type-F conjugative transfer system protein TrbI -
NHV94_RS00460 74805..77435 - 2631 WP_069067322 type IV secretion system protein TraC virb4
NHV94_RS00465 77562..77924 - 363 WP_069067323 hypothetical protein -
NHV94_RS00470 77952..78167 - 216 WP_000556747 hypothetical protein -
NHV94_RS00475 78247..78723 - 477 WP_069067324 hypothetical protein -
NHV94_RS00480 78787..79484 + 698 WP_094096600 IS1-like element IS1A family transposase -
NHV94_RS00485 79495..79713 - 219 WP_074447208 conjugal transfer protein TraR -
NHV94_RS00490 79848..80363 - 516 WP_001681800 type IV conjugative transfer system lipoprotein TraV traV
NHV94_RS00495 80360..80680 - 321 WP_069067340 conjugal transfer protein TrbD -
NHV94_RS00500 80667..81233 - 567 WP_001526038 conjugal transfer pilus-stabilizing protein TraP -
NHV94_RS00505 81223..82674 - 1452 WP_021503386 F-type conjugal transfer pilus assembly protein TraB traB
NHV94_RS00510 82674..83402 - 729 WP_001230809 type-F conjugative transfer system secretin TraK traK
NHV94_RS00515 83389..83955 - 567 WP_000399792 type IV conjugative transfer system protein TraE traE
NHV94_RS00520 83977..84288 - 312 WP_000012106 type IV conjugative transfer system protein TraL traL
NHV94_RS00525 84293..84655 - 363 WP_069067339 type IV conjugative transfer system pilin TraA -
NHV94_RS00530 84689..84916 - 228 WP_001254388 conjugal transfer relaxosome protein TraY -
NHV94_RS00535 85004..85696 - 693 WP_069067593 PAS domain-containing protein -
NHV94_RS00540 85890..86273 - 384 WP_069067309 conjugal transfer relaxosome DNA-binding protein TraM -
NHV94_RS00545 86604..87206 + 603 WP_000243712 transglycosylase SLT domain-containing protein virB1
NHV94_RS00550 87503..88324 - 822 WP_001234469 DUF932 domain-containing protein -
NHV94_RS00555 88444..88731 - 288 WP_033553574 hypothetical protein -
NHV94_RS00560 88756..88962 - 207 WP_033553572 hypothetical protein -
NHV94_RS00565 89032..89204 + 173 Protein_111 hypothetical protein -
NHV94_RS00570 89202..89432 - 231 WP_001426396 hypothetical protein -
NHV94_RS00575 89652..89777 - 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
NHV94_RS00580 89719..89868 - 150 Protein_114 plasmid maintenance protein Mok -
NHV94_RS00585 90090..90809 - 720 WP_033553968 plasmid SOS inhibition protein A -
NHV94_RS00590 90806..91240 - 435 WP_001470779 conjugation system SOS inhibitor PsiB -


Host bacterium


ID   14049 GenBank   NZ_CP099765
Plasmid name   S16BD04351|p106 Incompatibility group   IncFIB
Plasmid size   123529 bp Coordinate of oriT [Strand]   86511..86634 [-]
Host baterium   Salmonella enterica strain S16BD04351

Cargo genes


Drug resistance gene   blaCTX-M-15, mph(A), blaTEM-1B
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -