Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 113614 |
Name | oriT_S16BD04351|p106 |
Organism | Salmonella enterica strain S16BD04351 |
Sequence Completeness | - |
NCBI accession of oriT (coordinates [strand]) | NZ_CP099765 (86511..86634 [-], 124 nt) |
oriT length | 124 nt |
IRs (inverted repeats) | 92..99, 113..120 (ATAATGTA..TACATTAT) 90..95, 107..112 (AAATAA..TTATTT) 39..46, 49..56 (GCAAAAAC..GTTTTTGC) 3..10, 15..22 (TTGGTGGT..ACCACCAA) |
Location of nic site | _ |
Conserved sequence flanking the nic site |
_ |
Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
GGTTGGTGGTTCTCACCACCAAAAGCACCACACACTACGCAAAAACAAGTTTTTGCTGATTTGCTACTTGAATCATTAACTTATGTTTTAAATAATGTATTTTAATTTATTTTACATTATAAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 8723 | GenBank | WP_180566001 |
Name | traI_NHV94_RS00360_S16BD04351|p106 | UniProt ID | _ |
Length | 1756 a.a. | PDB ID | |
Note | Predicted by oriTfinder 2.0 |
Relaxase protein sequence
Download Length: 1756 a.a. Molecular weight: 191691.14 Da Isoelectric Point: 5.5476
MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQDPQLHTHVVVANVTQHNDEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAEWMQT
LKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELAMMAREQGREVQIIAADRRSQMNLKQDEGLSGELITGR
RQLQEGMVFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLSLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPASLPVSDSPFTALKL
ENGWVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVLESKNQAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRLEGTWAPEHSVTEFSHSQEAELAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWEKNPDALALVDSVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKPDREVMNAQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDCNGRSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDTQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAGQERDRADITEREAALPESVLRESQREQEAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
ID | 5108 | GenBank | WP_001254388 |
Name | WP_001254388_S16BD04351|p106 | UniProt ID | W9A0Z2 |
Length | 75 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
Auxiliary protein sequence
Download Length: 75 a.a. Molecular weight: 9072.20 Da Isoelectric Point: 10.2071
MRRRNARGGISRTVSVYLDEDTNNRLIRAKDRSGRSKTIEVQIRLRDHLKRFPDFYNEEIFREVIEENES
TFKEL
Protein domains
Predicted by InterproScan.
Protein structure
Source | ID | Structure |
---|---|---|
AlphaFold DB | W9A0Z2 |
ID | 5109 | GenBank | WP_069067309 |
Name | WP_069067309_S16BD04351|p106 | UniProt ID | _ |
Length | 127 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
Auxiliary protein sequence
Download Length: 127 a.a. Molecular weight: 14503.49 Da Isoelectric Point: 4.8498
MAKVQAYVSDEIVYKINKIVERRRAEGAKNTDVSFSSISTMLLELGLRVYETQMERKESAFNQTEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSSEMERFFPENDEG
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
ID | 10099 | GenBank | WP_180566002 |
Name | traD_NHV94_RS00365_S16BD04351|p106 | UniProt ID | _ |
Length | 726 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 726 a.a. Molecular weight: 82565.27 Da Isoelectric Point: 5.3214
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLILWVKISWQTFVNGCIYWWCTTLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYAR
KRGDMVVIYDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTYLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGESFTIRDWMRGVREDKKNGWLFISSNADTHASLKPVVSMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEYLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEGVTQAEQPQQPQQPQQPQQ
PQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQQENHPDIQQ
HMQRREEVNINVHRERGEDVEPGDDF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
ID | 10100 | GenBank | WP_069067322 |
Name | traC_NHV94_RS00460_S16BD04351|p106 | UniProt ID | _ |
Length | 876 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 876 a.a. Molecular weight: 99447.38 Da Isoelectric Point: 6.8589
MSKNPLETVTQAVNSLLTALKLPDESSQANQILGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAI
PINGANKSIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAA
ATQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIV
GEMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNPEIAF
LWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWG
ELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGK
GLFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSG
AGKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLS
VMASPNGNLDEVHEGLLLQAVKASWLAKKNQARIDDVVNFLKNASDSEQYAGSPTIRSRLDEMIVLLDQY
TANGTYGRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRTLKKLNVIDEGW
RLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKY
NQLFPDQFQPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREG
MSIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 58396..87206
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
NHV94_RS00365 | 58396..60576 | - | 2181 | WP_180566002 | type IV conjugative transfer system coupling protein TraD | virb4 |
NHV94_RS00370 | 60573..61352 | - | 780 | WP_069067317 | hypothetical protein | - |
NHV94_RS00375 | 61555..62286 | - | 732 | WP_000850429 | conjugal transfer complement resistance protein TraT | - |
NHV94_RS00380 | 62317..62820 | - | 504 | WP_000605509 | hypothetical protein | - |
NHV94_RS00385 | 62835..65657 | - | 2823 | WP_069067318 | conjugal transfer mating-pair stabilization protein TraG | traG |
NHV94_RS00390 | 65654..67027 | - | 1374 | WP_001137364 | conjugal transfer pilus assembly protein TraH | traH |
NHV94_RS00395 | 67014..67406 | - | 393 | WP_001744736 | F-type conjugal transfer protein TrbF | - |
NHV94_RS00400 | 67387..67728 | - | 342 | WP_001448115 | P-type conjugative transfer protein TrbJ | - |
NHV94_RS00405 | 67658..68203 | - | 546 | WP_000059832 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
NHV94_RS00410 | 68190..68474 | - | 285 | WP_000623730 | type-F conjugative transfer system pilin chaperone TraQ | - |
NHV94_RS00415 | 68601..68939 | - | 339 | WP_001287905 | conjugal transfer protein TrbA | - |
NHV94_RS00420 | 68955..69698 | - | 744 | WP_001030371 | type-F conjugative transfer system pilin assembly protein TraF | traF |
NHV94_RS00425 | 69691..69948 | - | 258 | WP_000864329 | conjugal transfer protein TrbE | - |
NHV94_RS00430 | 69975..71825 | - | 1851 | WP_000821864 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
NHV94_RS00435 | 71822..72460 | - | 639 | WP_001080257 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
NHV94_RS00440 | 72469..72774 | - | 306 | WP_069067320 | hypothetical protein | - |
NHV94_RS00445 | 72804..73796 | - | 993 | WP_069067321 | conjugal transfer pilus assembly protein TraU | traU |
NHV94_RS00450 | 73793..74425 | - | 633 | WP_001203727 | type-F conjugative transfer system protein TraW | traW |
NHV94_RS00455 | 74422..74808 | - | 387 | WP_000214082 | type-F conjugative transfer system protein TrbI | - |
NHV94_RS00460 | 74805..77435 | - | 2631 | WP_069067322 | type IV secretion system protein TraC | virb4 |
NHV94_RS00465 | 77562..77924 | - | 363 | WP_069067323 | hypothetical protein | - |
NHV94_RS00470 | 77952..78167 | - | 216 | WP_000556747 | hypothetical protein | - |
NHV94_RS00475 | 78247..78723 | - | 477 | WP_069067324 | hypothetical protein | - |
NHV94_RS00480 | 78787..79484 | + | 698 | WP_094096600 | IS1-like element IS1A family transposase | - |
NHV94_RS00485 | 79495..79713 | - | 219 | WP_074447208 | conjugal transfer protein TraR | - |
NHV94_RS00490 | 79848..80363 | - | 516 | WP_001681800 | type IV conjugative transfer system lipoprotein TraV | traV |
NHV94_RS00495 | 80360..80680 | - | 321 | WP_069067340 | conjugal transfer protein TrbD | - |
NHV94_RS00500 | 80667..81233 | - | 567 | WP_001526038 | conjugal transfer pilus-stabilizing protein TraP | - |
NHV94_RS00505 | 81223..82674 | - | 1452 | WP_021503386 | F-type conjugal transfer pilus assembly protein TraB | traB |
NHV94_RS00510 | 82674..83402 | - | 729 | WP_001230809 | type-F conjugative transfer system secretin TraK | traK |
NHV94_RS00515 | 83389..83955 | - | 567 | WP_000399792 | type IV conjugative transfer system protein TraE | traE |
NHV94_RS00520 | 83977..84288 | - | 312 | WP_000012106 | type IV conjugative transfer system protein TraL | traL |
NHV94_RS00525 | 84293..84655 | - | 363 | WP_069067339 | type IV conjugative transfer system pilin TraA | - |
NHV94_RS00530 | 84689..84916 | - | 228 | WP_001254388 | conjugal transfer relaxosome protein TraY | - |
NHV94_RS00535 | 85004..85696 | - | 693 | WP_069067593 | PAS domain-containing protein | - |
NHV94_RS00540 | 85890..86273 | - | 384 | WP_069067309 | conjugal transfer relaxosome DNA-binding protein TraM | - |
NHV94_RS00545 | 86604..87206 | + | 603 | WP_000243712 | transglycosylase SLT domain-containing protein | virB1 |
NHV94_RS00550 | 87503..88324 | - | 822 | WP_001234469 | DUF932 domain-containing protein | - |
NHV94_RS00555 | 88444..88731 | - | 288 | WP_033553574 | hypothetical protein | - |
NHV94_RS00560 | 88756..88962 | - | 207 | WP_033553572 | hypothetical protein | - |
NHV94_RS00565 | 89032..89204 | + | 173 | Protein_111 | hypothetical protein | - |
NHV94_RS00570 | 89202..89432 | - | 231 | WP_001426396 | hypothetical protein | - |
NHV94_RS00575 | 89652..89777 | - | 126 | WP_001372321 | type I toxin-antitoxin system Hok family toxin | - |
NHV94_RS00580 | 89719..89868 | - | 150 | Protein_114 | plasmid maintenance protein Mok | - |
NHV94_RS00585 | 90090..90809 | - | 720 | WP_033553968 | plasmid SOS inhibition protein A | - |
NHV94_RS00590 | 90806..91240 | - | 435 | WP_001470779 | conjugation system SOS inhibitor PsiB | - |
Host bacterium
ID | 14049 | GenBank | NZ_CP099765 |
Plasmid name | S16BD04351|p106 | Incompatibility group | IncFIB |
Plasmid size | 123529 bp | Coordinate of oriT [Strand] | 86511..86634 [-] |
Host baterium | Salmonella enterica strain S16BD04351 |
Cargo genes
Drug resistance gene | blaCTX-M-15, mph(A), blaTEM-1B |
Virulence gene | - |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | - |