Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113612
Name   oriT_S18BD02037|p127 in_silico
Organism   Salmonella enterica strain S18BD02037
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP099762 (233395..233483 [+], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_S18BD02037|p127
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   8721 GenBank   WP_006855641
Name   nikB_NHV97_RS01120_S18BD02037|p127 insolico UniProt ID   A0A620WL05
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104006.42 Da        Isoelectric Point: 7.3526

>WP_006855641.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A620WL05


Auxiliary protein

ID   5107 GenBank   WP_001283947
Name   WP_001283947_S18BD02037|p127 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   10098 GenBank   WP_001289275
Name   trbC_NHV97_RS01115_S18BD02037|p127 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86957.15 Da        Isoelectric Point: 6.7713

>WP_001289275.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHLTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 189128..228017

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NHV97_RS00890 184190..185257 + 1068 WP_001360287 type IV pilus biogenesis lipoprotein PilL -
NHV97_RS00895 185257..185694 + 438 WP_000539807 type IV pilus biogenesis protein PilM -
NHV97_RS00900 185708..187390 + 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
NHV97_RS00905 187383..188678 + 1296 Protein_188 type 4b pilus protein PilO2 -
NHV97_RS00910 188665..189117 + 453 WP_058649963 type IV pilus biogenesis protein PilP -
NHV97_RS00915 189128..190681 + 1554 WP_000362204 GspE/PulE family protein virB11
NHV97_RS00920 190694..191779 + 1086 WP_001208803 type II secretion system F family protein -
NHV97_RS00925 191796..192410 + 615 WP_000908228 type 4 pilus major pilin -
NHV97_RS00930 192420..192980 + 561 WP_000014005 lytic transglycosylase domain-containing protein virB1
NHV97_RS00935 192965..193621 + 657 WP_001193551 A24 family peptidase -
NHV97_RS00940 193621..194913 + 1293 WP_039719938 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
NHV97_RS00945 194910..195158 - 249 WP_001349157 hypothetical protein -
NHV97_RS01680 195539..195808 - 270 Protein_197 hypothetical protein -
NHV97_RS00950 195848..197002 + 1155 WP_001139955 tyrosine-type recombinase/integrase -
NHV97_RS00955 197153..197977 + 825 WP_058649947 conjugal transfer protein TraE traE
NHV97_RS00960 198063..199265 + 1203 WP_000976353 conjugal transfer protein TraF -
NHV97_RS00965 199325..199909 + 585 WP_000977520 histidine phosphatase family protein -
NHV97_RS00970 200304..200762 + 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
NHV97_RS00975 200759..201577 + 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
NHV97_RS00980 201574..202722 + 1149 WP_001024976 plasmid transfer ATPase TraJ virB11
NHV97_RS00985 202719..203009 + 291 WP_001299214 hypothetical protein traK
NHV97_RS00990 203024..203575 + 552 WP_000014584 phospholipase D family protein -
NHV97_RS00995 203665..207432 + 3768 WP_058649948 LPD7 domain-containing protein -
NHV97_RS01000 207450..207797 + 348 WP_001055900 conjugal transfer protein traL
NHV97_RS01005 207794..208486 + 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
NHV97_RS01010 208497..209480 + 984 WP_021513963 IncI1-type conjugal transfer protein TraN traN
NHV97_RS01015 209483..210772 + 1290 WP_001271994 conjugal transfer protein TraO traO
NHV97_RS01020 210772..211476 + 705 WP_023155244 IncI1-type conjugal transfer protein TraP traP
NHV97_RS01025 211476..212003 + 528 WP_001055569 conjugal transfer protein TraQ traQ
NHV97_RS01030 212054..212458 + 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
NHV97_RS01035 212522..212710 + 189 WP_001277255 putative conjugal transfer protein TraS -
NHV97_RS01040 212694..213494 + 801 WP_001164792 IncI1-type conjugal transfer protein TraT traT
NHV97_RS01045 213584..216628 + 3045 WP_001024776 IncI1-type conjugal transfer protein TraU traU
NHV97_RS01050 216628..217242 + 615 WP_000337399 IncI1-type conjugal transfer protein TraV traV
NHV97_RS01055 217209..218411 + 1203 WP_001189156 IncI1-type conjugal transfer protein TraW traW
NHV97_RS01060 218440..219024 + 585 WP_001037996 IncI1-type conjugal transfer protein TraX -
NHV97_RS01065 219121..221289 + 2169 WP_058649949 DotA/TraY family protein traY
NHV97_RS01070 221360..222022 + 663 WP_000644796 plasmid IncI1-type surface exclusion protein ExcA -
NHV97_RS01075 222094..222303 - 210 WP_000062603 HEAT repeat domain-containing protein -
NHV97_RS01080 222695..222871 + 177 WP_001054897 hypothetical protein -
NHV97_RS01645 222936..223031 - 96 WP_000609148 DinQ-like type I toxin DqlB -
NHV97_RS01090 223532..223783 + 252 WP_001291964 hypothetical protein -
NHV97_RS01095 223855..224007 - 153 WP_001387489 Hok/Gef family protein -
NHV97_RS01100 224634..225413 + 780 WP_275450201 protein FinQ -
NHV97_RS01105 225720..226928 + 1209 WP_001383960 IncI1-type conjugal transfer protein TrbA trbA
NHV97_RS01110 226947..228017 + 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
NHV97_RS01115 228010..230301 + 2292 WP_001289275 F-type conjugative transfer protein TrbC -

Host bacterium

ID   14047 GenBank   NZ_CP099762
Plasmid name   S18BD02037|p127 Incompatibility group   IncFIB
Plasmid size   318539 bp Coordinate of oriT [Strand]   233395..233483 [+]
Host baterium   Salmonella enterica strain S18BD02037

Cargo genes

Drug resistance gene   ant(3'')-Ia, qacE, sul1, tet(A), blaCTX-M-65, floR, aph(4)-Ia, aac(3)-IVa, dfrA14, aph(3')-Ia
Virulence gene   faeC, faeE, faeF, faeH, faeI, fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS
Metal resistance gene   merE, merD, merA, merP, merT, merR, arsH
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrVA2