Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   113604
Name   oriT_S16BD07964|p114 in_silico
Organism   Salmonella enterica strain S16BD07964
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP099764 (79260..79517 [-], 258 nt)
oriT length   258 nt
IRs (inverted repeats)      107..113, 127..133  (GCGGTGT..ACACCGC)
 19..26, 29..36  (GCAAAAAC..GTTTTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 258 nt

>oriT_S16BD07964|p114
AAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTTTCTTTATAAATAGAGAGTTATGACAAATTAGTTCTTCTTGCTCTCTTTGTGATATTTAAAAAAGCGGTGTCGGCGCAGCTACAACACCGCGCCGACACCGCTTTTTAGGGGTGGTACTGACTATTTTCATAAAAAACATCATCTTATATTAGGGGTGCTGCTAGCGGCGCGGTGTGTTTTTTTATAGGATACCGCCGGGGGCGCTGCTAGCGGTG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   8713 GenBank   WP_000986969
Name   traI_NHV95_RS00300_S16BD07964|p114 insolico UniProt ID   A0A731Y7R8
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 191917.73 Da        Isoelectric Point: 5.9718

>WP_000986969.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGKLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLQEGMAFTPGNTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGRPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSNSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVSDSPFMALKL
ENGWVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKTGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLLQRSGETPNFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWVPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
APVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKSDREVMNAERLFSTARELRDVVAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVQIARDNPDSGVVVRIAGE
GRPWNPGTITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVRDIAGLERDRSAISEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

(575-626)

(969-1156)

(10-283)

(1434-1557)

(633-712)


  Protein structure



No available structure.




Auxiliary protein


ID   5097 GenBank   WP_223195105
Name   WP_223195105_S16BD07964|p114 insolico UniProt ID   _
Length   133 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 133 a.a.        Molecular weight: 15423.73 Da        Isoelectric Point: 9.9240

>WP_223195105.1 MULTISPECIES: conjugal transfer relaxosome DNA-bindin protein TraY [Enterobacteriaceae]
MLLKRFGTRSATGKMVKLKLPLDVESLLIEASNRSGRSRSFEAVIRLKDHLHRYPKFNRAGNIYGKSLVK
YLTMRLDDETNQLLIAAKNRSGWCKTDEAADRVIDHLIKFPDFYNSEIFREADKEEDITFNTL

  Protein domains


Predicted by InterproScan.

(18-61)

(72-119)


  Protein structure



No available structure.



ID   5098 GenBank   WP_001151528
Name   WP_001151528_S16BD07964|p114 insolico UniProt ID   A0A731UEV6
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14577.51 Da        Isoelectric Point: 5.0517

>WP_001151528.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Enterobacteriaceae]
MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLELGLRVHEAQMERKESAFNQTEFNKLL
LEYVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSSEMERFFPKNDDE

  Protein domains


Predicted by InterproScan.

(1-126)


  Protein structure


Source ID Structure
AlphaFold DB A0A731UEV6


T4CP


ID   10088 GenBank   WP_000009324
Name   traD_NHV95_RS00315_S16BD07964|p114 insolico UniProt ID   _
Length   717 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 717 a.a.        Molecular weight: 81528.14 Da        Isoelectric Point: 5.1126

>WP_000009324.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIASMRIRMFSQIANIILYCLFIFFWILVGLVLWVKISWQTFVNGCIYWWCTTLEGMR
DLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQ
QSENEITGGRQLTDNPKEVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQTRPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPVSPAI
NDKKSDAGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAVYEAWQREQNPDIQQKMQRREEVN
INVHRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

(173-560)

(32-128)

  Protein structure



No available structure.



ID   10089 GenBank   WP_001064265
Name   traC_NHV95_RS00405_S16BD07964|p114 insolico UniProt ID   _
Length   875 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 875 a.a.        Molecular weight: 99296.07 Da        Isoelectric Point: 6.3032

>WP_001064265.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacteriaceae]
MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANESIVEALDHMLRTKLPRGVPFCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAA
TQFPLPEGMNLPLTLRHYRVFFSYCSPSKKKSRADILEMENLVKIIRASLQGASITTQAVDAQAFIDIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLREKGRNSTARILNFHLARNPEIAFL
WNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLAKKNRARIDDVVDFLKNARDNDQYVESPTIRSRLDEMIVLLDQYT
AGGTYGRYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGWR
LLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGL
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

(38-276)

(467-771)

(289-446)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 53651..80109

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NHV95_RS00305 53017..53244 + 228 WP_000450520 toxin-antitoxin system antitoxin VapB -
NHV95_RS00310 53244..53642 + 399 WP_000911313 type II toxin-antitoxin system VapC family toxin -
NHV95_RS00315 53651..55804 - 2154 WP_000009324 type IV conjugative transfer system coupling protein TraD virb4
NHV95_RS00320 56057..56788 - 732 WP_023565183 conjugal transfer complement resistance protein TraT -
NHV95_RS00325 56820..57317 - 498 WP_000605859 entry exclusion protein -
NHV95_RS00330 57333..60155 - 2823 WP_001007016 conjugal transfer mating-pair stabilization protein TraG traG
NHV95_RS00335 60152..61528 - 1377 WP_000982840 conjugal transfer pilus assembly protein TraH traH
NHV95_RS00340 61525..61905 - 381 WP_001366201 F-type conjugal transfer protein TrbF -
NHV95_RS00345 61892..62233 - 342 WP_001443031 P-type conjugative transfer protein TrbJ -
NHV95_RS00350 62163..62708 - 546 WP_000059851 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
NHV95_RS00355 62695..62979 - 285 WP_000624109 type-F conjugative transfer system pilin chaperone TraQ -
NHV95_RS00360 63060..63374 + 315 WP_000415566 protein ArtA -
NHV95_RS00365 63376..63723 - 348 WP_001287910 conjugal transfer protein TrbA -
NHV95_RS00370 63739..64482 - 744 WP_001030378 type-F conjugative transfer system pilin assembly protein TraF traF
NHV95_RS00375 64475..64732 - 258 WP_000864318 conjugal transfer protein TrbE -
NHV95_RS00380 64759..66567 - 1809 WP_000821840 type-F conjugative transfer system mating-pair stabilization protein TraN traN
NHV95_RS00385 66564..67202 - 639 WP_000777690 type-F conjugative transfer system pilin assembly protein TrbC trbC
NHV95_RS00390 67211..68203 - 993 WP_000830835 conjugal transfer pilus assembly protein TraU traU
NHV95_RS00395 68200..68832 - 633 WP_001203750 type-F conjugative transfer system protein TraW traW
NHV95_RS00400 68829..69215 - 387 WP_000099693 type-F conjugative transfer system protein TrbI -
NHV95_RS00405 69212..71839 - 2628 WP_001064265 type IV secretion system protein TraC virb4
NHV95_RS00410 71999..72220 - 222 WP_001278686 conjugal transfer protein TraR -
NHV95_RS00415 72355..72870 - 516 WP_000809885 type IV conjugative transfer system lipoprotein TraV traV
NHV95_RS00420 72867..73118 - 252 WP_001038331 conjugal transfer protein TrbG -
NHV95_RS00425 73111..73431 - 321 WP_001057304 conjugal transfer protein TrbD virb4
NHV95_RS00430 73418..73978 - 561 WP_000896605 conjugal transfer pilus-stabilizing protein TraP -
NHV95_RS00435 73968..75419 - 1452 WP_000146659 F-type conjugal transfer pilus assembly protein TraB traB
NHV95_RS00440 75419..76147 - 729 WP_001230805 type-F conjugative transfer system secretin TraK traK
NHV95_RS00445 76134..76700 - 567 WP_000399792 type IV conjugative transfer system protein TraE traE
NHV95_RS00450 76722..77033 - 312 WP_000012106 type IV conjugative transfer system protein TraL traL
NHV95_RS00455 77038..77400 - 363 WP_000994784 type IV conjugative transfer system pilin TraA -
NHV95_RS00460 77433..77828 - 396 WP_001366159 conjugal transfer relaxosome DNA-bindin protein TraY -
NHV95_RS00465 77927..78616 - 690 WP_000283383 conjugal transfer transcriptional regulator TraJ -
NHV95_RS00470 78803..79186 - 384 WP_001151528 conjugal transfer relaxosome DNA-binding protein TraM -
NHV95_RS00475 79507..80109 + 603 WP_000243710 transglycosylase SLT domain-containing protein virB1
NHV95_RS00480 80406..81227 - 822 WP_253075089 DUF932 domain-containing protein -
NHV95_RS00485 81347..81633 - 287 Protein_96 hypothetical protein -
NHV95_RS00490 81658..81864 - 207 WP_015059635 hypothetical protein -
NHV95_RS00495 81934..82107 + 174 Protein_98 hypothetical protein -
NHV95_RS00770 82105..82334 - 230 Protein_99 hypothetical protein -
NHV95_RS00500 82554..82679 - 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
NHV95_RS00505 82621..82770 - 150 Protein_101 plasmid maintenance protein Mok -
NHV95_RS00515 84231..84419 + 189 WP_001299721 hypothetical protein -


Host bacterium


ID   14039 GenBank   NZ_CP099764
Plasmid name   S16BD07964|p114 Incompatibility group   IncFIB
Plasmid size   127932 bp Coordinate of oriT [Strand]   79260..79517 [-]
Host baterium   Salmonella enterica strain S16BD07964

Cargo genes


Drug resistance gene   sul2, aph(3')-Ia, aph(6)-Id, aph(3'')-Ib, tet(B), blaCTX-M-32, sitABCD
Virulence gene   iutA, iucC, iucB, iucA
Metal resistance gene   yfeC
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -