Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 113604 |
Name | oriT_S16BD07964|p114 |
Organism | Salmonella enterica strain S16BD07964 |
Sequence Completeness | - |
NCBI accession of oriT (coordinates [strand]) | NZ_CP099764 (79260..79517 [-], 258 nt) |
oriT length | 258 nt |
IRs (inverted repeats) | 107..113, 127..133 (GCGGTGT..ACACCGC) 19..26, 29..36 (GCAAAAAC..GTTTTTGC) |
Location of nic site | _ |
Conserved sequence flanking the nic site |
_ |
Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 258 nt
AAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTTTCTTTATAAATAGAGAGTTATGACAAATTAGTTCTTCTTGCTCTCTTTGTGATATTTAAAAAAGCGGTGTCGGCGCAGCTACAACACCGCGCCGACACCGCTTTTTAGGGGTGGTACTGACTATTTTCATAAAAAACATCATCTTATATTAGGGGTGCTGCTAGCGGCGCGGTGTGTTTTTTTATAGGATACCGCCGGGGGCGCTGCTAGCGGTG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 8713 | GenBank | WP_000986969 |
Name | traI_NHV95_RS00300_S16BD07964|p114 | UniProt ID | A0A731Y7R8 |
Length | 1756 a.a. | PDB ID | |
Note | Predicted by oriTfinder 2.0 |
Relaxase protein sequence
Download Length: 1756 a.a. Molecular weight: 191917.73 Da Isoelectric Point: 5.9718
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGKLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLQEGMAFTPGNTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGRPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSNSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVSDSPFMALKL
ENGWVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKTGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLLQRSGETPNFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWVPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
APVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKSDREVMNAERLFSTARELRDVVAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVQIARDNPDSGVVVRIAGE
GRPWNPGTITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVRDIAGLERDRSAISEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
ID | 5097 | GenBank | WP_223195105 |
Name | WP_223195105_S16BD07964|p114 | UniProt ID | _ |
Length | 133 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
Auxiliary protein sequence
Download Length: 133 a.a. Molecular weight: 15423.73 Da Isoelectric Point: 9.9240
MLLKRFGTRSATGKMVKLKLPLDVESLLIEASNRSGRSRSFEAVIRLKDHLHRYPKFNRAGNIYGKSLVK
YLTMRLDDETNQLLIAAKNRSGWCKTDEAADRVIDHLIKFPDFYNSEIFREADKEEDITFNTL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
ID | 5098 | GenBank | WP_001151528 |
Name | WP_001151528_S16BD07964|p114 | UniProt ID | A0A731UEV6 |
Length | 127 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
Auxiliary protein sequence
Download Length: 127 a.a. Molecular weight: 14577.51 Da Isoelectric Point: 5.0517
MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLELGLRVHEAQMERKESAFNQTEFNKLL
LEYVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSSEMERFFPKNDDE
Protein domains
Predicted by InterproScan.
Protein structure
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A731UEV6 |
T4CP
ID | 10088 | GenBank | WP_000009324 |
Name | traD_NHV95_RS00315_S16BD07964|p114 | UniProt ID | _ |
Length | 717 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 717 a.a. Molecular weight: 81528.14 Da Isoelectric Point: 5.1126
MSFNAKDMTQGGQIASMRIRMFSQIANIILYCLFIFFWILVGLVLWVKISWQTFVNGCIYWWCTTLEGMR
DLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQ
QSENEITGGRQLTDNPKEVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQTRPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPVSPAI
NDKKSDAGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAVYEAWQREQNPDIQQKMQRREEVN
INVHRERGEDVEPGDDF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
ID | 10089 | GenBank | WP_001064265 |
Name | traC_NHV95_RS00405_S16BD07964|p114 | UniProt ID | _ |
Length | 875 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 875 a.a. Molecular weight: 99296.07 Da Isoelectric Point: 6.3032
MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANESIVEALDHMLRTKLPRGVPFCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAA
TQFPLPEGMNLPLTLRHYRVFFSYCSPSKKKSRADILEMENLVKIIRASLQGASITTQAVDAQAFIDIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLREKGRNSTARILNFHLARNPEIAFL
WNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLAKKNRARIDDVVDFLKNARDNDQYVESPTIRSRLDEMIVLLDQYT
AGGTYGRYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGWR
LLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGL
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 53651..80109
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
NHV95_RS00305 | 53017..53244 | + | 228 | WP_000450520 | toxin-antitoxin system antitoxin VapB | - |
NHV95_RS00310 | 53244..53642 | + | 399 | WP_000911313 | type II toxin-antitoxin system VapC family toxin | - |
NHV95_RS00315 | 53651..55804 | - | 2154 | WP_000009324 | type IV conjugative transfer system coupling protein TraD | virb4 |
NHV95_RS00320 | 56057..56788 | - | 732 | WP_023565183 | conjugal transfer complement resistance protein TraT | - |
NHV95_RS00325 | 56820..57317 | - | 498 | WP_000605859 | entry exclusion protein | - |
NHV95_RS00330 | 57333..60155 | - | 2823 | WP_001007016 | conjugal transfer mating-pair stabilization protein TraG | traG |
NHV95_RS00335 | 60152..61528 | - | 1377 | WP_000982840 | conjugal transfer pilus assembly protein TraH | traH |
NHV95_RS00340 | 61525..61905 | - | 381 | WP_001366201 | F-type conjugal transfer protein TrbF | - |
NHV95_RS00345 | 61892..62233 | - | 342 | WP_001443031 | P-type conjugative transfer protein TrbJ | - |
NHV95_RS00350 | 62163..62708 | - | 546 | WP_000059851 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
NHV95_RS00355 | 62695..62979 | - | 285 | WP_000624109 | type-F conjugative transfer system pilin chaperone TraQ | - |
NHV95_RS00360 | 63060..63374 | + | 315 | WP_000415566 | protein ArtA | - |
NHV95_RS00365 | 63376..63723 | - | 348 | WP_001287910 | conjugal transfer protein TrbA | - |
NHV95_RS00370 | 63739..64482 | - | 744 | WP_001030378 | type-F conjugative transfer system pilin assembly protein TraF | traF |
NHV95_RS00375 | 64475..64732 | - | 258 | WP_000864318 | conjugal transfer protein TrbE | - |
NHV95_RS00380 | 64759..66567 | - | 1809 | WP_000821840 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
NHV95_RS00385 | 66564..67202 | - | 639 | WP_000777690 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
NHV95_RS00390 | 67211..68203 | - | 993 | WP_000830835 | conjugal transfer pilus assembly protein TraU | traU |
NHV95_RS00395 | 68200..68832 | - | 633 | WP_001203750 | type-F conjugative transfer system protein TraW | traW |
NHV95_RS00400 | 68829..69215 | - | 387 | WP_000099693 | type-F conjugative transfer system protein TrbI | - |
NHV95_RS00405 | 69212..71839 | - | 2628 | WP_001064265 | type IV secretion system protein TraC | virb4 |
NHV95_RS00410 | 71999..72220 | - | 222 | WP_001278686 | conjugal transfer protein TraR | - |
NHV95_RS00415 | 72355..72870 | - | 516 | WP_000809885 | type IV conjugative transfer system lipoprotein TraV | traV |
NHV95_RS00420 | 72867..73118 | - | 252 | WP_001038331 | conjugal transfer protein TrbG | - |
NHV95_RS00425 | 73111..73431 | - | 321 | WP_001057304 | conjugal transfer protein TrbD | virb4 |
NHV95_RS00430 | 73418..73978 | - | 561 | WP_000896605 | conjugal transfer pilus-stabilizing protein TraP | - |
NHV95_RS00435 | 73968..75419 | - | 1452 | WP_000146659 | F-type conjugal transfer pilus assembly protein TraB | traB |
NHV95_RS00440 | 75419..76147 | - | 729 | WP_001230805 | type-F conjugative transfer system secretin TraK | traK |
NHV95_RS00445 | 76134..76700 | - | 567 | WP_000399792 | type IV conjugative transfer system protein TraE | traE |
NHV95_RS00450 | 76722..77033 | - | 312 | WP_000012106 | type IV conjugative transfer system protein TraL | traL |
NHV95_RS00455 | 77038..77400 | - | 363 | WP_000994784 | type IV conjugative transfer system pilin TraA | - |
NHV95_RS00460 | 77433..77828 | - | 396 | WP_001366159 | conjugal transfer relaxosome DNA-bindin protein TraY | - |
NHV95_RS00465 | 77927..78616 | - | 690 | WP_000283383 | conjugal transfer transcriptional regulator TraJ | - |
NHV95_RS00470 | 78803..79186 | - | 384 | WP_001151528 | conjugal transfer relaxosome DNA-binding protein TraM | - |
NHV95_RS00475 | 79507..80109 | + | 603 | WP_000243710 | transglycosylase SLT domain-containing protein | virB1 |
NHV95_RS00480 | 80406..81227 | - | 822 | WP_253075089 | DUF932 domain-containing protein | - |
NHV95_RS00485 | 81347..81633 | - | 287 | Protein_96 | hypothetical protein | - |
NHV95_RS00490 | 81658..81864 | - | 207 | WP_015059635 | hypothetical protein | - |
NHV95_RS00495 | 81934..82107 | + | 174 | Protein_98 | hypothetical protein | - |
NHV95_RS00770 | 82105..82334 | - | 230 | Protein_99 | hypothetical protein | - |
NHV95_RS00500 | 82554..82679 | - | 126 | WP_001372321 | type I toxin-antitoxin system Hok family toxin | - |
NHV95_RS00505 | 82621..82770 | - | 150 | Protein_101 | plasmid maintenance protein Mok | - |
NHV95_RS00515 | 84231..84419 | + | 189 | WP_001299721 | hypothetical protein | - |
Host bacterium
ID | 14039 | GenBank | NZ_CP099764 |
Plasmid name | S16BD07964|p114 | Incompatibility group | IncFIB |
Plasmid size | 127932 bp | Coordinate of oriT [Strand] | 79260..79517 [-] |
Host baterium | Salmonella enterica strain S16BD07964 |
Cargo genes
Drug resistance gene | sul2, aph(3')-Ia, aph(6)-Id, aph(3'')-Ib, tet(B), blaCTX-M-32, sitABCD |
Virulence gene | iutA, iucC, iucB, iucA |
Metal resistance gene | yfeC |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | - |