Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113553
Name   oriT_pSSE2 in_silico
Organism   Shigella sonnei strain 500867
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KP979588 (1053..1227 [+], 175 nt)
oriT length   175 nt
IRs (inverted repeats)      101..106, 120..125  (ACAATC..GATTGT)
Location of nic site      65..66
Conserved sequence flanking the
  nic site    		  ACTGGCATAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 175 nt

>oriT_pSSE2
GTGACTCCGTGCGCGGTGAAAAATCGCATTTTAGCGCGTCACTGGTAGTTGCAAAACTGAACTGGCATAATGCACGGCACATCACGAAGTGCGCACTTATACAATCTCTACTCCGTTTCGATTGTGTGCGGTCTGCGACGCTAAAAGAAAACGGCAAAAAGGTATTACGGCAGAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   8685 GenBank   WP_052979306
Name   mobQ_HPF10_RS00015_pSSE2 insolico UniProt ID   A0A0G3EZY5
Length   739 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 739 a.a.        Molecular weight: 84368.62 Da        Isoelectric Point: 8.2796

>WP_052979306.1 MULTISPECIES: MobQ family relaxase [Enterobacteriaceae]
MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTRKRGVDDAVILTPANAPSWCSDRSVLW
NAVEKAEQRRNSQLAREIELAIPREISREAAREAVLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTT
RAVGETGFAGKVRDWNERALAETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAM
EKRGMETERGEQNRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLR
EFIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASLLSEIPLMTWA
EPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFETQWNTWIAPGMAELKEKLSAREAERRREE
AEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELGRAREPGTGRITRYMMLSNRAGEFTVWGDELAH
YPQSVHDPVNVYLSPGGAVMVSDIREGMPESHETMARPERMRMYSGATVRHVLEQMRQGWPSYGFPALPH
HWPDNFYFSDDRRPVASPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREG
GFCPVLVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAAVHKPEE
MPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(18-213)


  Protein structure


Source ID Structure
AlphaFold DB A0A0G3EZY5


Host bacterium

ID   13988 GenBank   NZ_KP979588
Plasmid name   pSSE2 Incompatibility group   Col156
Plasmid size   6728 bp Coordinate of oriT [Strand]   1053..1227 [+]
Host baterium   Shigella sonnei strain 500867

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -