Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113542
Name   oriT_EF62pB in_silico
Organism   Enterococcus faecalis 62
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NC_017313 (30553..30602 [+], 50 nt)
oriT length   50 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_EF62pB
AATATCGCAACATGCTAGCATGTTGCTCTGCTCGCAAAAAGAAAGCCTAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   8679 GenBank   WP_002367932
Name   Relaxase_EF62_RS15575_EF62pB insolico UniProt ID   R3K157
Length   561 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 561 a.a.        Molecular weight: 65400.15 Da        Isoelectric Point: 6.0174

>WP_002367932.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterococcus]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKIAAKEGAKIIEKSPKNS
HKKYTMWETESLYKEKIKSRLDFLLEQSSSINDFLEKAEALNLSVDFSKKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIVERLKENKSVLTLKEVVEKYVEKSEKSKNEFDYQLTIDEWQISHKTTRGYYVNVDFG
FGERGKVFIGAYKVDPLENGQYNIYVKRKDFFYLMNEKNADRNRYMTGETLIKQLRLYNGQTPLKKEPVM
RTIDELVNAINFLAANEIEDTRQLELLEEKLEAAFLEAEETLETLDEKMLELHQLSNLLLENELNGELEL
IQQKLKTLLPDATLAEFSYEDVRGEIEAIKTSQSLLENKLERTRNEINQLHEIQAVQKKETANQNNVKPK
L

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(37-296)


  Protein structure


Source ID Structure
AlphaFold DB R3K157


Auxiliary protein

ID   5068 GenBank   WP_002362408
Name   WP_002362408_EF62pB insolico UniProt ID   A0A1J6XY92
Length   118 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 118 a.a.        Molecular weight: 13979.04 Da        Isoelectric Point: 9.6545

>WP_002362408.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Bacillota]
MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKEQS

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(64-105)


  Protein structure


Source ID Structure
AlphaFold DB A0A1J6XY92


T4CP

ID   10035 GenBank   WP_002386494
Name   t4cp2_EF62_RS15555_EF62pB insolico UniProt ID   _
Length   609 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 609 a.a.        Molecular weight: 70117.08 Da        Isoelectric Point: 6.1807

>WP_002386494.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQTYKKELKPYLYLGVFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
AGVLGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(144-558)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 10622..27908

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EF62_RS15450 (EF62_pB0006) 5845..6225 + 381 WP_014524900 hypothetical protein -
EF62_RS16375 (EF62_pB0007) 6275..6496 + 222 WP_002385566 hypothetical protein -
EF62_RS15460 (EF62_pB0008) 6796..7101 + 306 WP_002375285 hypothetical protein -
EF62_RS15465 (EF62_pB0009) 7112..9676 + 2565 WP_014524901 surface exclusion protein SEA1/PrgA -
EF62_RS15470 (EF62_pB0010) 9755..9949 + 195 WP_014524902 hypothetical protein -
EF62_RS16380 (EF62_pB0011) 10222..10410 + 189 Protein_9 hypothetical protein -
EF62_RS15480 (EF62_pB0012) 10622..14539 + 3918 WP_014524903 LPXTG-anchored aggregation substance PrgB/Asc10 prgB
EF62_RS15485 (EF62_pB0013) 14601..14957 + 357 WP_002367672 pheromone response system RNA-binding regulator PrgU -
EF62_RS15490 (EF62_pB0014) 14985..15842 + 858 WP_002367673 pheromone response LPXTG-anchored adhesin PrgC prgC
EF62_RS15495 (EF62_pB0015) 15884..16783 + 900 WP_002409287 hypothetical protein -
EF62_RS15500 (EF62_pB0016) 16803..17237 + 435 WP_002365857 hypothetical protein -
EF62_RS15505 (EF62_pB0017) 17284..17511 + 228 WP_002365858 hypothetical protein -
EF62_RS15510 (EF62_pB0018) 17524..17820 + 297 WP_002382798 hypothetical protein -
EF62_RS15515 (EF62_pB0019) 17835..18635 + 801 WP_002382799 type IV secretion system protein prgHb
EF62_RS15520 (EF62_pB0020) 18637..18990 + 354 WP_014524904 PrgI family protein prgIc
EF62_RS15525 (EF62_pB0021) 18944..21334 + 2391 WP_045009097 VirB4-like conjugal transfer ATPase, CD1110 family virb4
EF62_RS15530 (EF62_pB0022) 21346..23961 + 2616 WP_014524906 phage tail tip lysozyme prgK
EF62_RS15535 (EF62_pB0023) 23985..24611 + 627 WP_045009098 hypothetical protein prgL
EF62_RS15540 (EF62_pB0024) 24598..24843 + 246 WP_002365866 hypothetical protein -
EF62_RS15545 (EF62_pB0025) 24827..25435 + 609 WP_010715953 hypothetical protein -
EF62_RS15550 (EF62_pB0026) 25594..26079 + 486 WP_002386493 PcfB family protein gbs1365
EF62_RS15555 (EF62_pB0027) 26079..27908 + 1830 WP_002386494 VirD4-like conjugal transfer protein, CD1115 family virb4
EF62_RS15560 (EF62_pB0028) 27959..30118 + 2160 WP_014524908 ArdC-like ssDNA-binding domain-containing protein -
EF62_RS15565 (EF62_pB0029) 30151..30423 + 273 WP_002367930 LtrD -
EF62_RS15570 (EF62_pB0030) 30669..31025 + 357 WP_002362408 plasmid mobilization relaxosome protein MobC -
EF62_RS15575 (EF62_pB0031) 31026..32711 + 1686 WP_002367932 relaxase/mobilization nuclease domain-containing protein -

Host bacterium

ID   13977 GenBank   NC_017313
Plasmid name   EF62pB Incompatibility group   -
Plasmid size   51104 bp Coordinate of oriT [Strand]   30553..30602 [+]
Host baterium   Enterococcus faecalis 62

Cargo genes

Drug resistance gene   -
Virulence gene   prgB/asc10
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21, AcrIIA9