Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113425
Name   oriT_pWM1B in_silico
Organism   Lactococcus lactis subsp. lactis strain WM1
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP032502 (48439..48575 [+], 137 nt)
oriT length   137 nt
IRs (inverted repeats)      21..28, 42..49  (AAAAGGGG..CCCCTTTT)
 25..30, 39..44  (GGGGAA..TTCCCC)
Location of nic site      104..105
Conserved sequence flanking the
  nic site    		  GCTTGCAGTA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 137 nt

>oriT_pWM1B
CGACACAATCCAAAGGGGATAAAAGGGGAAAGTAAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAATCACTTCGTTCTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   8603 GenBank   WP_240205153
Name   Relaxase_LLWM1_RS13395_pWM1B insolico UniProt ID   _
Length   410 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 47667.24 Da        Isoelectric Point: 8.1264

>WP_240205153.1 relaxase/mobilization nuclease domain-containing protein [Lactococcus lactis]
MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGIDPFIAKEQFD
VIRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLETGNKLREQKGESVQRAREMNDRLASRENWHILEPPKERQTETEKELIAKNEYSYMDDLRE
RINKSLQDVSVSSYETFKERLSDNGVILSERGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENR
ARQNEFSAVEQREPAITPLERDTQQRESEIVSLEQAIEPRKSEALKRESKINRFIDTIKQLAGRVPQLTQ
RVTRKLKQTKEKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-280)


  Protein structure



No available structure.




Host bacterium

ID   13860 GenBank   NZ_CP032502
Plasmid name   pWM1B Incompatibility group   -
Plasmid size   51875 bp Coordinate of oriT [Strand]   48439..48575 [+]
Host baterium   Lactococcus lactis subsp. lactis strain WM1

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   cadX
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -