Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113418
Name   oriT_pIncI1-ST26 in_silico
Organism   Salmonella enterica subsp. enterica serovar Heidelberg isolate FT2-21BPWGent
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP128391 (41409..41495 [-], 87 nt)
oriT length   87 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 87 nt

>oriT_pIncI1-ST26
GGGGTGTCGGGGCGAAGCCCTGACCAGGAGGCAATTGTATAATCGCGCGTGCGCGGTTATACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   8598 GenBank   WP_001354015
Name   nikB_QS243_RS23590_pIncI1-ST26 insolico UniProt ID   E8XLT1
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104018.47 Da        Isoelectric Point: 7.3526

>WP_001354015.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCLAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPENRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB E8XLT1


Auxiliary protein

ID   2376 GenBank   WP_001281051
Name   WP_001281051_pIncI1-ST26 insolico UniProt ID   A0A3U8I0C6
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12646.44 Da        Isoelectric Point: 10.4030

>WP_001281051.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSDKRQRSGSERRQKTILRAVRFSPDEDETICKNAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A3U8I0C6


T4CP

ID   9935 GenBank   WP_001289276
Name   trbC_QS243_RS23595_pIncI1-ST26 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 46874..76304

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QS243_RS23595 (QS243_23595) 44590..46881 - 2292 WP_001289276 F-type conjugative transfer protein TrbC -
QS243_RS23600 (QS243_23600) 46874..47944 - 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
QS243_RS23605 (QS243_23605) 47963..49171 - 1209 WP_000121274 IncI1-type conjugal transfer protein TrbA trbA
QS243_RS23610 (QS243_23610) 49463..49615 + 153 WP_001331364 Hok/Gef family protein -
QS243_RS24225 49687..49878 - 192 WP_174715448 hypothetical protein -
QS243_RS23615 (QS243_23615) 50439..50534 + 96 WP_000609148 DinQ-like type I toxin DqlB -
QS243_RS23620 (QS243_23620) 50599..50775 - 177 WP_001054904 hypothetical protein -
QS243_RS23625 (QS243_23625) 51167..51376 + 210 WP_000062603 HEAT repeat domain-containing protein -
QS243_RS23630 (QS243_23630) 51448..52110 - 663 WP_000653334 plasmid IncI1-type surface exclusion protein ExcA -
QS243_RS23635 (QS243_23635) 52175..54337 - 2163 WP_000698351 DotA/TraY family protein traY
QS243_RS23640 (QS243_23640) 54434..55018 - 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
QS243_RS23645 (QS243_23645) 55047..56249 - 1203 WP_001189160 IncI1-type conjugal transfer protein TraW traW
QS243_RS23650 (QS243_23650) 56216..56830 - 615 WP_000337398 IncI1-type conjugal transfer protein TraV traV
QS243_RS23655 (QS243_23655) 56830..59874 - 3045 WP_001024780 IncI1-type conjugal transfer protein TraU traU
QS243_RS23660 (QS243_23660) 59964..60764 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
QS243_RS23665 (QS243_23665) 60748..60936 - 189 WP_001277255 putative conjugal transfer protein TraS -
QS243_RS23670 (QS243_23670) 61000..61404 - 405 WP_000086957 IncI1-type conjugal transfer protein TraR traR
QS243_RS23675 (QS243_23675) 61455..61982 - 528 WP_001055569 conjugal transfer protein TraQ traQ
QS243_RS23680 (QS243_23680) 61982..62686 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
QS243_RS23685 (QS243_23685) 62686..63975 - 1290 WP_001272005 conjugal transfer protein TraO traO
QS243_RS23690 (QS243_23690) 63978..64961 - 984 WP_001191877 IncI1-type conjugal transfer protein TraN traN
QS243_RS23695 (QS243_23695) 64972..65664 - 693 WP_000138548 DotI/IcmL family type IV secretion protein traM
QS243_RS23700 (QS243_23700) 65661..66008 - 348 WP_001055900 conjugal transfer protein traL
QS243_RS23705 (QS243_23705) 66026..69793 - 3768 WP_001141529 LPD7 domain-containing protein -
QS243_RS23710 (QS243_23710) 69883..70434 - 552 WP_000014583 phospholipase D family protein -
QS243_RS23715 (QS243_23715) 70449..70739 - 291 WP_001299214 hypothetical protein traK
QS243_RS23720 (QS243_23720) 70736..71884 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
QS243_RS23725 (QS243_23725) 71881..72699 - 819 WP_000646098 IncI1-type conjugal transfer lipoprotein TraI traI
QS243_RS23730 (QS243_23730) 72696..73154 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
QS243_RS23735 (QS243_23735) 73549..74133 - 585 WP_000977522 histidine phosphatase family protein -
QS243_RS23740 (QS243_23740) 74193..75395 - 1203 WP_000976351 conjugal transfer protein TraF -
QS243_RS23745 (QS243_23745) 75480..76304 - 825 WP_001238939 conjugal transfer protein TraE traE
QS243_RS23750 (QS243_23750) 76455..77609 - 1155 WP_001139957 tyrosine-type recombinase/integrase -

Host bacterium

ID   13853 GenBank   NZ_CP128391
Plasmid name   pIncI1-ST26 Incompatibility group   -
Plasmid size   77659 bp Coordinate of oriT [Strand]   41409..41495 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Heidelberg isolate FT2-21BPWGent

Cargo genes

Drug resistance gene   aac(3)-VIa, ant(3'')-Ia
Virulence gene   -
Metal resistance gene   merR1, merT, merP, merA
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -