Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113338
Name   oriT_S11_VPH_Chula|unnamed2 in_silico
Organism   Salmonella enterica strain S11_VPH_Chula
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP121388 (19423..21129 [-], 1707 nt)
oriT length   1707 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 1707 nt

>oriT_S11_VPH_Chula|unnamed2
GCCGTATGAATGACATCTATTTCCGTCCAATTCATCGAAACCAGTATTTTGAGGAAATGAAAGCATGATAAAGCAAATTAAAGTAATCATGTTGGCAGCAATAGTTACCGGATGTTCATCGCCGCCGCCTCCCGTTCCTGTGGAGTGGGATAAAGCCGCTGACCCATTAAACACCAGTTTACCGCAATGGAGTGACAATAACGTTATAGTCCCTTCCCCGACAGTTAACGGTAAATGGACATCATCAATCAGCGCACAAAGTTTCTACGATACAATATGGACTCCAGCAGTATTTTACGCAGTCGCCCATTCAACACGAATTGTCGTCACTGCACAATCCGGCACTGATTTCTTTAATGCCAAAAACTGGCTTCGCCAGAATGGCGCAAAAGGCGTTATTGAATACCAGCCTGTCTTTAATTGCCTGACGTGCAGAGAAACCACTATTTATTTCTCTCGCTAATCCATTTAAGCAACACAATCTAATAAGGATTTATCATGAAAAAAACCGTTATCGCGGCGGCTGTATTATGCCTGACCGCAGGAACGCCGATTTATTCCTTTGCCGCTGACCCGTGCGAAGTTGTTCTTTGCATGTACGGAAAAGCTACAGGTAATAGTGGTGGGAGTGAATGCAGTTCAGCAGAACGCGCATTTTTTAAAATAAACGCATTTAAAAAACACCATCGTTTCAATCCGGGTAAAACATTCGATATGCGAAAAGAATTTTTAGGGGAGTGCAAAGCCGCAGACCCTGAATCAATAAGCAAAATCCTTTCAAAATTTGGTAGAGTGAAAGGATAACTGACATATCCATTTTTATATGAGATGTTCGGAATGATAAATGAAAATTTATTCATTAAGAATATACACTCAAAAAATCAGGACAGAATATCTGTAGCCCTGGTATATGACACGTTATCAAAAGAAGCCCACAGAGGTTGTGGGCTGTATTACGAAATATACGAAAGTTGTTTTATCGGTCTTTTGCGCGATCATCTTTCAGAACTTAATGAGGTTGACGCAAACAAATTAATACGCTATGCCGAAAGCAAAGGCACAAAAATTGATGATGCGTCCTACAGTGAAGCACTGGAAGCTGAAAGGGAGTGCCGGGCTGAAATATACCGCGAGCAAATGTAATCACACAAAACAAAAGTGTATTGTACAATACACAGACTAACAGGCTACGGGATTTTAAGGCTTCATTATTTTTGGCTTCAATTTCAAAGAAATTGTTAAGACGAAAAATAATGAAGTGGTACGCGGAAAGAAAATTCAATTTCGGAGAAATTGCTAATTTTCAGGAACCGCGCCCACCTGCGAACATTCGCCCACAGCGAATTGAGCAGGCCGGGGGGAGCCGCAAAAATTTTGCCGCCCCAGCGAGCAAAAAGGCACGCCCACGTGCCGTTTTTTGTGGGGGGCTTGCCCCGCACACCGTCGCAGCCAGGTAATATAACCCGCCCCAGCGGGGGATATTACCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCGATTTTTTCGAGCTTGCGAGGAAAAATAGGCTAAACGCAGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCAAGACGGTGTGCCGCCTTTGACCTTGGGGGGTTTTGGCTCGGCGTCCAGCCGGGACATTACCCCCATGTGTCATGCAGTGAGGGCGTCCAGCCCGAACGTAATGGCACACGCCGT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


T4CP

ID   9874 GenBank   WP_094309419
Name   t4cp2_QAT90_RS24740_S11_VPH_Chula|unnamed2 insolico UniProt ID   _
Length   578 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 578 a.a.        Molecular weight: 65670.93 Da        Isoelectric Point: 7.4566

>WP_094309419.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLLAGLTLPFIFHLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKSLDNNILFRVPLWIMTIIFTAAGLIFHIAVRRLLAGEIDNLKHRMIKKSKLERNT
RTDVRKVKELLPDSIEYNPLDYIDLKKGVFIGLNKDDQPQYITIKEFKTQHAAIIGTTGSGKGVTATVLL
YQAILLGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKLNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLRDLFNTDFVQSLREDVPAFCGELEEIALVNSINATKGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDSVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKVDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTSTELPRATKMFQVQVKKENIELLSFEYK
QATPEKNESQEHKPSINL

  Protein domains


Predicted by InterproScan.

Helicase HerA, central domain (DUF87)

(174-280)

TraM recognition site of TraD and TraG (TraG-D_C)

(399-490)

  Protein structure



No available structure.



ID   9875 GenBank   WP_012457110
Name   t4cp2_QAT90_RS24795_S11_VPH_Chula|unnamed2 insolico UniProt ID   _
Length   912 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 912 a.a.        Molecular weight: 103056.99 Da        Isoelectric Point: 7.4625

>WP_012457110.1 MULTISPECIES: VirB3 family type IV secretion system protein [Enterobacterales]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGSAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

  Protein domains


Predicted by InterproScan.

Type IV secretory pathway, VirB3-like protein (VirB3)

(3-79)

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(269-470)

TraG P-loop domain (P-loop_TraG)

(535-813)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 17470..31019

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QAT90_RS24705 (QAT90_24705) 12845..13195 + 351 WP_012457127 type II toxin-antitoxin system RelE/ParE family toxin -
QAT90_RS24710 (QAT90_24710) 13198..13497 + 300 WP_012457126 helix-turn-helix domain-containing protein -
QAT90_RS24715 (QAT90_24715) 13559..13708 - 150 WP_162262154 hypothetical protein -
QAT90_RS24720 (QAT90_24720) 13871..14536 - 666 WP_094309421 hypothetical protein -
QAT90_RS24725 (QAT90_24725) 14921..15889 + 969 WP_012457123 ArdC family protein -
QAT90_RS24730 (QAT90_24730) 16073..16603 - 531 WP_012457122 phospholipase D family protein -
QAT90_RS24735 (QAT90_24735) 16706..17461 - 756 WP_023316386 MobC family replication-relaxation protein -
QAT90_RS24740 (QAT90_24740) 17470..19206 - 1737 WP_094309419 type IV secretory system conjugative DNA transfer family protein virb4
QAT90_RS24745 (QAT90_24745) 19987..20292 - 306 WP_094309429 dpoa decarboxylase -
QAT90_RS24750 (QAT90_24750) 20326..20631 - 306 WP_012457118 hypothetical protein -
QAT90_RS24755 (QAT90_24755) 20667..21065 - 399 WP_012457117 cag pathogenicity island Cag12 family protein -
QAT90_RS24760 (QAT90_24760) 21062..22087 - 1026 WP_012457116 P-type DNA transfer ATPase VirB11 virB11
QAT90_RS24765 (QAT90_24765) 22077..23345 - 1269 WP_139968916 VirB10/TraB/TrbI family type IV secretion system protein virB10
QAT90_RS24770 (QAT90_24770) 23392..24300 - 909 WP_012457114 P-type conjugative transfer protein VirB9 virB9
QAT90_RS24775 (QAT90_24775) 24297..24980 - 684 WP_094309417 type IV secretion system protein virB8
QAT90_RS24780 (QAT90_24780) 25202..26278 - 1077 WP_012457112 type IV secretion system protein virB6
QAT90_RS24785 (QAT90_24785) 26290..26517 - 228 WP_004178109 EexN family lipoprotein -
QAT90_RS24790 (QAT90_24790) 26527..27237 - 711 WP_012457111 type IV secretion system protein -
QAT90_RS24795 (QAT90_24795) 27256..29994 - 2739 WP_012457110 VirB3 family type IV secretion system protein virb4
QAT90_RS24800 (QAT90_24800) 30007..30312 - 306 WP_012457109 TrbC/VirB2 family protein virB2
QAT90_RS24805 (QAT90_24805) 30309..31019 - 711 WP_004178175 lytic transglycosylase domain-containing protein virB1
QAT90_RS24810 (QAT90_24810) 31018..31140 + 123 WP_255093128 hypothetical protein -
QAT90_RS24815 (QAT90_24815) 31137..31355 - 219 WP_032722781 hypothetical protein -
QAT90_RS24820 (QAT90_24820) 31360..31575 - 216 WP_004178170 hypothetical protein -
QAT90_RS24825 (QAT90_24825) 31647..32102 - 456 WP_009309974 transcription termination/antitermination NusG family protein -
QAT90_RS24830 (QAT90_24830) 32356..32715 + 360 WP_012457108 helix-turn-helix transcriptional regulator -
QAT90_RS24835 (QAT90_24835) 33179..33880 + 702 WP_258549312 RepA family replication protein -
QAT90_RS24840 (QAT90_24840) 34745..35074 + 330 WP_000493286 type II toxin-antitoxin system RelE/ParE family toxin -
QAT90_RS24845 (QAT90_24845) 35055..35336 + 282 WP_000780222 helix-turn-helix transcriptional regulator -
QAT90_RS24850 (QAT90_24850) 35599..35907 + 309 WP_353958078 hypothetical protein -

Host bacterium

ID   13773 GenBank   NZ_CP121388
Plasmid name   S11_VPH_Chula|unnamed2 Incompatibility group   IncFII
Plasmid size   38472 bp Coordinate of oriT [Strand]   19423..21129 [-]
Host baterium   Salmonella enterica strain S11_VPH_Chula

Cargo genes

Drug resistance gene   qnrS1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -