Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113332
Name   oriT_S10_VPH_Chula|unnamed2 in_silico
Organism   Salmonella enterica strain S10_VPH_Chula
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP121394 (17344..19050 [+], 1707 nt)
oriT length   1707 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 1707 nt

>oriT_S10_VPH_Chula|unnamed2
GCCGTATGAATGACATCTATTTCCGTCCAATTCATCGAAACCAGTATTTTGAGGAAATGAAAGCATGATAAAGCAAATTAAAGTAATCATGTTGGCAGCAATAGTTACCGGATGTTCATCGCCGCCGCCTCCCGTTCCTGTGGAGTGGGATAAAGCCGCTGACCCATTAAACACCAGTTTACCGCAATGGAGTGACAATAACGTTATAGTCCCTTCCCCGACAGTTAACGGTAAATGGACATCATCAATCAGCGCACAAAGTTTCTACGATACAATATGGACTCCAGCAGTATTTTACGCAGTCGCCCATTCAACACGAATTGTCGTCACTGCACAATCCGGCACTGATTTCTTTAATGCCAAAAACTGGCTTCGCCAGAATGGCGCAAAAGGCGTTATTGAATACCAGCCTGTCTTTAATTGCCTGACGTGCAGAGAAACCACTATTTATTTCTCTCGCTAATCCATTTAAGCAACACAATCTAATAAGGATTTATCATGAAAAAAACCGTTATCGCGGCGGCTGTATTATGCCTGACCGCAGGAACGCCGATTTATTCCTTTGCCGCTGACCCGTGCGAAGTTGTTCTTTGCATGTACGGAAAAGCTACAGGTAATAGTGGTGGGAGTGAATGCAGTTCAGCAGAACGCGCATTTTTTAAAATAAACGCATTTAAAAAACACCATCGTTTCAATCCGGGTAAAACATTCGATATGCGAAAAGAATTTTTAGGGGAGTGCAAAGCCGCAGACCCTGAATCAATAAGCAAAATCCTTTCAAAATTTGGTAGAGTGAAAGGATAACTGACATATCCATTTTTATATGAGATGTTCGGAATGATAAATGAAAATTTATTCATTAAGAATATACACTCAAAAAATCAGGACAGAATATCTGTAGCCCTGGTATATGACACGTTATCAAAAGAAGCCCACAGAGGTTGTGGGCTGTATTACGAAATATACGAAAGTTGTTTTATCGGTCTTTTGCGCGATCATCTTTCAGAACTTAATGAGGTTGACGCAAACAAATTAATACGCTATGCCGAAAGCAAAGGCACAAAAATTGATGATGCGTCCTACAGTGAAGCACTGGAAGCTGAAAGGGAGTGCCGGGCTGAAATATACCGCGAGCAAATGTAATCACACAAAACAAAAGTGTATTGTACAATACACAGACTAACAGGCTACGGGATTTTAAGGCTTCATTATTTTTGGCTTCAATTTCAAAGAAATTGTTAAGACGAAAAATAATGAAGTGGTACGCGGAAAGAAAATTCAATTTCGGAGAAATTGCTAATTTTCAGGAACCGCGCCCACCTGCGAACATTCGCCCACAGCGAATTGAGCAGGCCGGGGGGAGCCGCAAAAATTTTGCCGCCCCAGCGAGCAAAAAGGCACGCCCACGTGCCGTTTTTTGTGGGGGGCTTGCCCCGCACACCGTCGCAGCCAGGTAATATAACCCGCCCCAGCGGGGGATATTACCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCGATTTTTTCGAGCTTGCGAGGAAAAATAGGCTAAACGCAGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCAAGACGGTGTGCCGCCTTTGACCTTGGGGGGTTTTGGCTCGGCGTCCAGCCGGGACATTACCCCCATGTGTCATGCAGTGAGGGCGTCCAGCCCGAACGTAATGGCACACGCCGT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


T4CP

ID   9868 GenBank   WP_012457110
Name   t4cp2_QAT98_RS24730_S10_VPH_Chula|unnamed2 insolico UniProt ID   _
Length   912 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 912 a.a.        Molecular weight: 103056.99 Da        Isoelectric Point: 7.4625

>WP_012457110.1 MULTISPECIES: VirB3 family type IV secretion system protein [Enterobacterales]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGSAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

  Protein domains


Predicted by InterproScan.

Type IV secretory pathway, VirB3-like protein (VirB3)

(3-79)

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(269-470)

TraG P-loop domain (P-loop_TraG)

(535-813)

  Protein structure



No available structure.



ID   9869 GenBank   WP_094309419
Name   t4cp2_QAT98_RS24785_S10_VPH_Chula|unnamed2 insolico UniProt ID   _
Length   578 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 578 a.a.        Molecular weight: 65670.93 Da        Isoelectric Point: 7.4566

>WP_094309419.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLLAGLTLPFIFHLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKSLDNNILFRVPLWIMTIIFTAAGLIFHIAVRRLLAGEIDNLKHRMIKKSKLERNT
RTDVRKVKELLPDSIEYNPLDYIDLKKGVFIGLNKDDQPQYITIKEFKTQHAAIIGTTGSGKGVTATVLL
YQAILLGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKLNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLRDLFNTDFVQSLREDVPAFCGELEEIALVNSINATKGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDSVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKVDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTSTELPRATKMFQVQVKKENIELLSFEYK
QATPEKNESQEHKPSINL

  Protein domains


Predicted by InterproScan.

Helicase HerA, central domain (DUF87)

(174-280)

TraM recognition site of TraD and TraG (TraG-D_C)

(399-490)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 7454..21003

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QAT98_RS24675 (QAT98_24675) 2566..2874 - 309 WP_353958078 hypothetical protein -
QAT98_RS24680 (QAT98_24680) 3137..3418 - 282 WP_000780222 helix-turn-helix transcriptional regulator -
QAT98_RS24685 (QAT98_24685) 3399..3728 - 330 WP_000493286 type II toxin-antitoxin system RelE/ParE family toxin -
QAT98_RS24690 (QAT98_24690) 4593..5294 - 702 WP_258549312 RepA family replication protein -
QAT98_RS24695 (QAT98_24695) 5758..6117 - 360 WP_012457108 helix-turn-helix transcriptional regulator -
QAT98_RS24700 (QAT98_24700) 6371..6826 + 456 WP_009309974 transcription termination/antitermination NusG family protein -
QAT98_RS24705 (QAT98_24705) 6898..7113 + 216 WP_004178170 hypothetical protein -
QAT98_RS24710 (QAT98_24710) 7118..7336 + 219 WP_032722781 hypothetical protein -
QAT98_RS24715 (QAT98_24715) 7333..7455 - 123 WP_255093128 hypothetical protein -
QAT98_RS24720 (QAT98_24720) 7454..8164 + 711 WP_004178175 lytic transglycosylase domain-containing protein virB1
QAT98_RS24725 (QAT98_24725) 8161..8466 + 306 WP_012457109 TrbC/VirB2 family protein virB2
QAT98_RS24730 (QAT98_24730) 8479..11217 + 2739 WP_012457110 VirB3 family type IV secretion system protein virb4
QAT98_RS24735 (QAT98_24735) 11236..11946 + 711 WP_012457111 type IV secretion system protein -
QAT98_RS24740 (QAT98_24740) 11956..12183 + 228 WP_004178109 EexN family lipoprotein -
QAT98_RS24745 (QAT98_24745) 12195..13271 + 1077 WP_012457112 type IV secretion system protein virB6
QAT98_RS24750 (QAT98_24750) 13493..14176 + 684 WP_094309417 type IV secretion system protein virB8
QAT98_RS24755 (QAT98_24755) 14173..15081 + 909 WP_012457114 P-type conjugative transfer protein VirB9 virB9
QAT98_RS24760 (QAT98_24760) 15128..16396 + 1269 WP_139968916 VirB10/TraB/TrbI family type IV secretion system protein virB10
QAT98_RS24765 (QAT98_24765) 16386..17411 + 1026 WP_012457116 P-type DNA transfer ATPase VirB11 virB11
QAT98_RS24770 (QAT98_24770) 17408..17806 + 399 WP_012457117 cag pathogenicity island Cag12 family protein -
QAT98_RS24775 (QAT98_24775) 17842..18147 + 306 WP_012457118 hypothetical protein -
QAT98_RS24780 (QAT98_24780) 18181..18486 + 306 WP_094309429 dpoa decarboxylase -
QAT98_RS24785 (QAT98_24785) 19267..21003 + 1737 WP_094309419 type IV secretory system conjugative DNA transfer family protein virb4
QAT98_RS24790 (QAT98_24790) 21012..21767 + 756 WP_023316386 MobC family replication-relaxation protein -
QAT98_RS24795 (QAT98_24795) 21870..22400 + 531 WP_012457122 phospholipase D family protein -
QAT98_RS24800 (QAT98_24800) 22584..23552 - 969 WP_012457123 ArdC family protein -
QAT98_RS24805 (QAT98_24805) 23937..24602 + 666 WP_094309421 hypothetical protein -
QAT98_RS24810 (QAT98_24810) 24765..24914 + 150 WP_162262154 hypothetical protein -
QAT98_RS24815 (QAT98_24815) 24976..25275 - 300 WP_012457126 helix-turn-helix domain-containing protein -
QAT98_RS24820 (QAT98_24820) 25278..25628 - 351 WP_012457127 type II toxin-antitoxin system RelE/ParE family toxin -

Host bacterium

ID   13767 GenBank   NZ_CP121394
Plasmid name   S10_VPH_Chula|unnamed2 Incompatibility group   IncFII
Plasmid size   38472 bp Coordinate of oriT [Strand]   17344..19050 [+]
Host baterium   Salmonella enterica strain S10_VPH_Chula

Cargo genes

Drug resistance gene   qnrS1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -