Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 113291
• Name: oriT_KF1|unnamed3
• Organism: Bacillus thuringiensis strain KF1
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP085412 (4497..4528 [-], 32 nt)
• oriT length: 32 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 32 nt

>oriT_KF1|unnamed3
ATAGTAAAGTATAGTGTGCTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_LIT28_RS30040_KF1|unnamed3

• ID: 8525
• GenBank: WP_252209376
• Name: mobV_LIT28_RS30040_KF1|unnamed3
• UniProt ID: _
• Length: 408 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 408 a.a.
• Molecular weight: 48071.85 Da
• Isoelectric Point: 9.4636

>WP_252209376.1 MULTISPECIES: MobV family relaxase [Bacillus]
MSYAICRMQKFKMRDVKGIQIHNQREKESHTNHDIESERTHLNYDLHNDGHIDYLKTVKDKIEQNVEINR
AIRKDAVVMCEFVVTSDREFFDVLDMVDPAQQKVFFKEAYSFLKERYDERNIVHAAVHLDEKTPHMHVGM
VPVTEENKLSAKQIFNRKELVSLQDDFHAHMVEKGFYLERGVSSDKKHVEMRKFKAMTAKEEINALEKEI
GQCLQKKEVVNKQMEQLESRLNQLQNAVNSTNKVDKIEVKHKGGILRSKTVEMSVEDFEELKTKAKASEA
FKRENMTLHRKNEALQASNTDLHTTLKRLEKENEGLKPYKGKYERLAKLFDEMNKFYEKFIPKEVSKFHE
IIGFCKRKVNASINRFSSFRYSEKALNENEKKGYEAASKFLATQQKQQRKERGNEREL

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-196)

Protein structure

No available structure.

Host bacterium

• ID: 13726
• GenBank: NZ_CP085412
• Plasmid name: KF1|unnamed3
• Incompatibility group: -
• Plasmid size: 5764 bp
• Coordinate of oriT [Strand]: 4497..4528 [-]
• Host baterium: Bacillus thuringiensis strain KF1

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -