Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113222
Name   oriT_IncFIB/IncFIC in_silico
Organism   Salmonella enterica strain PIW95_S14_0299
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP117035 (75473..75824 [-], 352 nt)
oriT length   352 nt
IRs (inverted repeats)      255..262, 271..278  (ACCGCTAG..CTAGCGGT)
 192..198, 206..212  (TATAAAA..TTTTATA)
 40..47, 50..57  (GCAAAAAC..GTTTTTGC)
 4..11, 16..23  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 352 nt

>oriT_IncFIB/IncFIC
AGGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTTTCTTTATAAATAGAGAGTTATGAAAAATTAGTTTCTCTTACTCTCTTTATGATATTTAAAAAAGCGGTGTCGGCGCGGCTACAACAACGCGCCGACACCGCTTTGTAGGGGTGGTACTGACTATTTTTATAAAAAACATTATTTTATATTAGGGGTGCTGCTAGCGGCGCGGTGTGTTTTTTTATAGGATACCGCTAGGGGCGCTGCTAGCGGTGCGTCCCTGTTTGCATTATGAATTTTAGTGTTTCGAAATTAACTTTATTTTATGTTCAAAAAAGGTAATCTCTA

Visualization of oriT structure



Relaxase

ID   8483 GenBank   WP_054377139
Name   traI_PQP07_RS25965_IncFIB/IncFIC insolico UniProt ID   A0A5W1N632
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 191884.75 Da        Isoelectric Point: 6.1058

>WP_054377139.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHTVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNSYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVTSVSEDAMTVVVPGRAEPASLPVADSPFTALKL
ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGEILLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTSFTDLGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESGRPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALVLVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGAGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSGRQQTRVIRPAQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGSRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVF
EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETVRRADEIVRKMAENKPDLP
DSKTEQAVREIAGQERDRSAISEREAALPESVLRESQREREAVREIARENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)

AAA domain (AAA_30)

(969-1156)

DNA helicase TraI (TraI_C)

(1434-1557)

TrwC relaxase (TrwC)

(10-283)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-626)


  Protein structure



No available structure.




Auxiliary protein

ID   4881 GenBank   WP_001369361
Name   WP_001369361_IncFIB/IncFIC insolico UniProt ID   B7LI64
Length   133 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 133 a.a.        Molecular weight: 15409.70 Da        Isoelectric Point: 9.9240

>WP_001369361.1 MULTISPECIES: conjugal transfer relaxosome DNA-bindin protein TraY [Gammaproteobacteria]
MLLKRFGTRSATGKMVKLKLPVDVESLLIEASNRSGRSRSFEAVIRLKDHLHRYPKFNRAGNIYGKSLVK
YLTMRLDDETNQLLIAAKNRSGWCKTDEAADRVIDHLIKFPDFYNSEIFREADKEEDITFNTL

  Protein domains


Predicted by InterproScan.

TraY domain (TraY)

(18-61)

TraY domain (TraY)

(72-119)


  Protein structure


Source ID Structure
AlphaFold DB B7LI64

ID   4882 GenBank   WP_001151524
Name   WP_001151524_IncFIB/IncFIC insolico UniProt ID   A0A5W2DT22
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14517.48 Da        Isoelectric Point: 5.0516

>WP_001151524.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Gammaproteobacteria]
MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLELGLRVHEAQMERKESAFNQTEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSSEMERFFPKNDDE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-126)


  Protein structure


Source ID Structure
AlphaFold DB A0A5W2DT22


T4CP

ID   9787 GenBank   WP_001064265
Name   traC_PQP07_RS26065_IncFIB/IncFIC insolico UniProt ID   _
Length   875 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 875 a.a.        Molecular weight: 99296.07 Da        Isoelectric Point: 6.3032

>WP_001064265.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacteriaceae]
MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANESIVEALDHMLRTKLPRGVPFCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAA
TQFPLPEGMNLPLTLRHYRVFFSYCSPSKKKSRADILEMENLVKIIRASLQGASITTQAVDAQAFIDIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLREKGRNSTARILNFHLARNPEIAFL
WNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLAKKNRARIDDVVDFLKNARDNDQYVESPTIRSRLDEMIVLLDQYT
AGGTYGRYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGWR
LLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGL
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

TraC protein (TraC_F_IV)

(38-276)

TraG P-loop domain (P-loop_TraG)

(467-771)

Family of unknown function (DUF5934) (DUF5934)

(289-446)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 54850..76395

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PQP07_RS25985 (PQP07_25995) 50226..51839 + 1614 WP_000080195 IS66-like element ISEc23 family transposase -
PQP07_RS25990 (PQP07_26000) 51868..53316 - 1449 Protein_58 type IV conjugative transfer system coupling protein TraD -
PQP07_RS25995 (PQP07_26005) 53569..54300 - 732 WP_000850424 conjugal transfer complement resistance protein TraT -
PQP07_RS26000 (PQP07_26010) 54332..54835 - 504 WP_000605510 hypothetical protein -
PQP07_RS26005 (PQP07_26015) 54850..57672 - 2823 WP_001007048 conjugal transfer mating-pair stabilization protein TraG traG
PQP07_RS26010 (PQP07_26020) 57669..59045 - 1377 WP_000982842 conjugal transfer pilus assembly protein TraH traH
PQP07_RS26015 (PQP07_26025) 59042..59404 - 363 WP_001443292 P-type conjugative transfer protein TrbJ -
PQP07_RS26020 (PQP07_26030) 59334..59879 - 546 WP_000052613 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
PQP07_RS26025 (PQP07_26035) 59866..60147 - 282 WP_039002206 type-F conjugative transfer system pilin chaperone TraQ -
PQP07_RS26030 (PQP07_26040) 60263..61006 - 744 WP_039002205 type-F conjugative transfer system pilin assembly protein TraF traF
PQP07_RS26035 (PQP07_26045) 60999..61259 - 261 WP_000864348 conjugal transfer protein TrbE -
PQP07_RS26040 (PQP07_26050) 61286..63094 - 1809 WP_000821844 type-F conjugative transfer system mating-pair stabilization protein TraN traN
PQP07_RS26045 (PQP07_26055) 63091..63729 - 639 WP_000777691 type-F conjugative transfer system pilin assembly protein TrbC trbC
PQP07_RS26050 (PQP07_26060) 63738..64730 - 993 WP_023148235 conjugal transfer pilus assembly protein TraU traU
PQP07_RS26055 (PQP07_26065) 64727..65359 - 633 WP_001749440 type-F conjugative transfer system protein TraW traW
PQP07_RS26060 (PQP07_26070) 65356..65742 - 387 WP_039002198 type-F conjugative transfer system protein TrbI -
PQP07_RS26065 (PQP07_26075) 65739..68366 - 2628 WP_001064265 type IV secretion system protein TraC virb4
PQP07_RS26070 (PQP07_26080) 68526..68747 - 222 WP_001278692 conjugal transfer protein TraR -
PQP07_RS26075 (PQP07_26085) 68882..69397 - 516 WP_000724019 type IV conjugative transfer system lipoprotein TraV traV
PQP07_RS26080 (PQP07_26090) 69394..69714 - 321 WP_001057285 conjugal transfer protein TrbD -
PQP07_RS26085 (PQP07_26095) 69701..70288 - 588 WP_000002772 conjugal transfer pilus-stabilizing protein TraP -
PQP07_RS26090 (PQP07_26100) 70278..71705 - 1428 WP_000146690 F-type conjugal transfer pilus assembly protein TraB traB
PQP07_RS26095 (PQP07_26105) 71705..72433 - 729 WP_001230787 type-F conjugative transfer system secretin TraK traK
PQP07_RS26100 (PQP07_26110) 72420..72986 - 567 WP_000399792 type IV conjugative transfer system protein TraE traE
PQP07_RS26105 (PQP07_26115) 73008..73319 - 312 WP_000012106 type IV conjugative transfer system protein TraL traL
PQP07_RS26110 (PQP07_26120) 73324..73686 - 363 WP_000994784 type IV conjugative transfer system pilin TraA -
PQP07_RS26115 (PQP07_26125) 73719..74114 - 396 WP_001309237 conjugal transfer relaxosome DNA-bindin protein TraY -
PQP07_RS26120 (PQP07_26130) 74213..74902 - 690 WP_039002187 conjugal transfer transcriptional regulator TraJ -
PQP07_RS26125 (PQP07_26135) 75089..75472 - 384 WP_001151524 conjugal transfer relaxosome DNA-binding protein TraM -
PQP07_RS26130 (PQP07_26140) 75805..76395 + 591 WP_001376243 transglycosylase SLT domain-containing protein virB1
PQP07_RS26135 (PQP07_26145) 76692..77513 - 822 WP_001234469 DUF932 domain-containing protein -
PQP07_RS26140 (PQP07_26150) 77635..77922 - 288 WP_000107535 hypothetical protein -
PQP07_RS26145 (PQP07_26155) 77947..78153 - 207 WP_000547968 hypothetical protein -
PQP07_RS26150 (PQP07_26160) 78223..78396 + 174 Protein_90 hypothetical protein -
PQP07_RS26155 (PQP07_26165) 78394..78624 - 231 WP_001426396 hypothetical protein -
PQP07_RS26160 (PQP07_26170) 78844..78969 - 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
PQP07_RS26165 (PQP07_26175) 78911..79060 - 150 Protein_93 plasmid maintenance protein Mok -
PQP07_RS26170 (PQP07_26180) 79282..80001 - 720 WP_001276232 plasmid SOS inhibition protein A -
PQP07_RS26175 (PQP07_26185) 79998..80432 - 435 WP_000845953 conjugation system SOS inhibitor PsiB -

Host bacterium

ID   13657 GenBank   NZ_CP117035
Plasmid name   IncFIB/IncFIC Incompatibility group   IncFIB
Plasmid size   133901 bp Coordinate of oriT [Strand]   75473..75824 [-]
Host baterium   Salmonella enterica strain PIW95_S14_0299

Cargo genes

Drug resistance gene   aph(3')-Ia, tet(M), dfrA12, aadA2, cmlA1, ant(3'')-Ia, sul3, mef(B), blaTEM-1B
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -