Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113128
Name   oriT_HH-P049|pla2 in_silico
Organism   Escherichia sp. HH154_1D strain HH-P049
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_AP025753 (36239..36327 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_HH-P049|pla2
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   8427 GenBank   WP_001350015
Name   nikB_QUE53_RS27805_HH-P049|pla2 insolico UniProt ID   E0J389
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104034.43 Da        Isoelectric Point: 7.3526

>WP_001350015.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB E0J389


Auxiliary protein

ID   4837 GenBank   WP_001283947
Name   WP_001283947_HH-P049|pla2 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   9712 GenBank   WP_072119433
Name   trbC_QUE53_RS27810_HH-P049|pla2 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86925.06 Da        Isoelectric Point: 6.7713

>WP_072119433.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPEPPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 41705..79415

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE53_RS27810 (EsCd1HHP049_05569) 39421..41712 - 2292 WP_072119433 F-type conjugative transfer protein TrbC -
QUE53_RS27815 (EsCd1HHP049_05570) 41705..42775 - 1071 WP_000151580 IncI1-type conjugal transfer protein TrbB trbB
QUE53_RS27820 (EsCd1HHP049_05571) 42794..44002 - 1209 WP_001303305 IncI1-type conjugal transfer protein TrbA trbA
QUE53_RS27825 44294..44446 + 153 WP_001303307 Hok/Gef family protein -
QUE53_RS27830 (EsCd1HHP049_05572) 44518..44769 - 252 WP_001291967 hypothetical protein -
QUE53_RS27835 45268..45363 + 96 WP_001303310 DinQ-like type I toxin DqlB -
QUE53_RS27840 (EsCd1HHP049_05573) 45428..45604 - 177 WP_001054898 hypothetical protein -
QUE53_RS27845 (EsCd1HHP049_05574) 45936..46145 + 210 WP_000062602 HEAT repeat domain-containing protein -
QUE53_RS27850 (EsCd1HHP049_05575) 46217..46879 - 663 WP_000653333 plasmid IncI1-type surface exclusion protein ExcA -
QUE53_RS27855 (EsCd1HHP049_05576) 46944..49106 - 2163 WP_032220734 DotA/TraY family protein traY
QUE53_RS27860 (EsCd1HHP049_05577) 49203..49787 - 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
QUE53_RS27865 (EsCd1HHP049_05578) 49816..51018 - 1203 WP_001189156 IncI1-type conjugal transfer protein TraW traW
QUE53_RS27870 50985..51599 - 615 WP_000337399 IncI1-type conjugal transfer protein TraV traV
QUE53_RS27875 (EsCd1HHP049_05579) 51599..54643 - 3045 WP_001024780 IncI1-type conjugal transfer protein TraU traU
QUE53_RS27880 (EsCd1HHP049_05580) 54733..55533 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
QUE53_RS27885 (EsCd1HHP049_05581) 55517..55705 - 189 WP_001277255 putative conjugal transfer protein TraS -
QUE53_RS27890 (EsCd1HHP049_05582) 55769..56173 - 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
QUE53_RS27895 (EsCd1HHP049_05583) 56224..56751 - 528 WP_001055569 conjugal transfer protein TraQ traQ
QUE53_RS27900 (EsCd1HHP049_05584) 56751..57461 - 711 WP_286347571 IncI1-type conjugal transfer protein TraP traP
QUE53_RS27905 (EsCd1HHP049_05585) 57461..58750 - 1290 WP_001272005 conjugal transfer protein TraO traO
QUE53_RS27910 (EsCd1HHP049_05586) 58753..59736 - 984 WP_001191877 IncI1-type conjugal transfer protein TraN traN
QUE53_RS27915 59747..60439 - 693 WP_000138548 DotI/IcmL family type IV secretion protein traM
QUE53_RS27920 (EsCd1HHP049_05588) 60436..60783 - 348 WP_001055900 conjugal transfer protein traL
QUE53_RS27925 (EsCd1HHP049_05589) 60801..64568 - 3768 WP_103900478 LPD7 domain-containing protein -
QUE53_RS27930 (EsCd1HHP049_05590) 64659..65210 - 552 WP_000014584 phospholipase D family protein -
QUE53_RS27935 65225..65515 - 291 WP_001314267 hypothetical protein traK
QUE53_RS27940 (EsCd1HHP049_05591) 65512..66660 - 1149 WP_001024971 plasmid transfer ATPase TraJ virB11
QUE53_RS27945 (EsCd1HHP049_05592) 66657..67475 - 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
QUE53_RS27950 (EsCd1HHP049_05593) 67472..67930 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
QUE53_RS27955 (EsCd1HHP049_05594) 68324..68908 - 585 WP_000977522 histidine phosphatase family protein -
QUE53_RS27960 (EsCd1HHP049_05595) 68968..70170 - 1203 WP_000976353 conjugal transfer protein TraF -
QUE53_RS27965 70255..71079 - 825 WP_001238939 conjugal transfer protein TraE traE
QUE53_RS27970 (EsCd1HHP049_05597) 71230..72384 - 1155 WP_074516522 tyrosine-type recombinase/integrase -
QUE53_RS27975 (EsCd1HHP049_05598) 72383..72700 + 318 WP_001558195 hypothetical protein -
QUE53_RS27980 (EsCd1HHP049_05601) 73372..73620 + 249 WP_001349157 hypothetical protein -
QUE53_RS27985 (EsCd1HHP049_05602) 73617..74909 - 1293 WP_001417545 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
QUE53_RS27990 74909..75565 - 657 WP_001193553 prepilin peptidase -
QUE53_RS27995 75550..76110 - 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
QUE53_RS28000 (EsCd1HHP049_05603) 76120..76734 - 615 WP_001542527 type 4 pilus major pilin -
QUE53_RS28005 (EsCd1HHP049_05604) 76752..77849 - 1098 WP_001492931 type II secretion system F family protein -
QUE53_RS28010 (EsCd1HHP049_05605) 77862..79415 - 1554 WP_001384241 GspE/PulE family protein virB11
QUE53_RS28015 (EsCd1HHP049_05606) 79426..79878 - 453 WP_089520140 type IV pilus biogenesis protein PilP -
QUE53_RS28020 (EsCd1HHP049_05607) 79865..81160 - 1296 WP_286347572 type 4b pilus protein PilO2 -
QUE53_RS28025 (EsCd1HHP049_05608) 81153..82835 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
QUE53_RS28030 (EsCd1HHP049_05609) 82849..83286 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
QUE53_RS28035 83286..84353 - 1068 WP_012783947 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   13563 GenBank   NZ_AP025753
Plasmid name   HH-P049|pla2 Incompatibility group   IncI
Plasmid size   91132 bp Coordinate of oriT [Strand]   36239..36327 [-]
Host baterium   Escherichia sp. HH154_1D strain HH-P049

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -