Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113099
Name   oriT_10290|pla2 in_silico
Organism   Escherichia sp. 10290
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_AP025743 (54007..54079 [-], 73 nt)
oriT length   73 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 73 nt

>oriT_10290|pla2
GTCGGGGCAAAGCCCTGACCAGGTGGGGAATGTCTGAGTGCGCGTGCGCGGTCCGACATTCCCACATCCTGTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   8408 GenBank   WP_000991386
Name   Relaxase_QUE74_RS28970_10290|pla2 insolico UniProt ID   A0A648F3C8
Length   903 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 903 a.a.        Molecular weight: 104553.93 Da        Isoelectric Point: 7.5347

>WP_000991386.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacteriaceae]
MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDVPHRNRFRRLVDYATRLRNEKFVSLID
VMKDGSQWVNFYGVTCFHNCNSLETAAEEMQYKADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVR
HTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCF
VHAPGNRIVRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGE
LVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETE
SLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVIPGERFRGRNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGY
TTVQCESLIEDMIAGGNFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEP
QAGPFEIAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLVWKEHWRK
PDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYP
LSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNADRCVILCEPGGTPLYEDTGVLEARLQKDGS
VRFRDRRNGELVCVDYGDRVVFYHHQDRNELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAW
HNAAGITGNGHFVISRPDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-315)


  Protein structure


Source ID Structure
AlphaFold DB A0A648F3C8


Auxiliary protein

ID   4816 GenBank   WP_001291058
Name   WP_001291058_10290|pla2 insolico UniProt ID   A0A7I0L241
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12730.52 Da        Isoelectric Point: 9.9656

>WP_001291058.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVSTFIRSSSLDKVVNCRTDDRMIDEIMRL
GRLQKHLFVEGKRTGDKEYAEVLVAITEYVNALRRDLMGR

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A7I0L241


T4CP

ID   9686 GenBank   WP_286332282
Name   trbC_QUE74_RS28985_10290|pla2 insolico UniProt ID   _
Length   767 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 767 a.a.        Molecular weight: 87060.25 Da        Isoelectric Point: 7.7106

>WP_286332282.1 F-type conjugative transfer protein TrbC [Escherichia sp. 10290]
MSQHHVNPDLIHRTAWGNPLWNALHNLNIIGLCLAGSIITALIWPLALPVCLLFTLVTSVIFTLQRWRCP
LRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQYEADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHVM
FFATTGGGKTETTFAWLLNPLSWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEI
IQSGEKSRPQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVRERKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWTEEPRKQHAYLSGQFSE
TFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTSAHTTSALGRMFVTQQSMILARDLGYRLE
GTDAQTLEVKKYKGSFPYICILDEVGAYYTDRIAVEATQVRSLEFSLIMMAQDQERIEGQTSATNTATLM
QNAGTKFAGRIVSDDKTARTVKNAAGEEARARMGSLLRHDGVMGESWVDGNQITIQMESKIDVQDLIRLN
AGEFFTVFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPEHVSSII
GVLTAKPSRKRRKNRTEPYRIIDTFQRRLATAQTSLDLLPQYDTDIESRANELWKKAVHTINNTTREERR
VCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPDLTASPPHKKNVAQTPSGGKQESRKKRF

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(439-520)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 79565..112668

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE74_RS29060 (EsCdI10290_05762) 76116..76358 - 243 WP_000064387 hypothetical protein -
QUE74_RS29065 (EsCdI10290_05763) 76691..76843 + 153 WP_001597021 Hok/Gef family protein -
QUE74_RS29070 (EsCdI10290_05764) 76981..78567 - 1587 WP_000004565 TnsA endonuclease N-terminal domain-containing protein -
QUE74_RS29075 (EsCdI10290_05765) 78841..79494 - 654 WP_001098361 plasmid IncI1-type surface exclusion protein ExcA -
QUE74_RS29080 (EsCdI10290_05766) 79565..81730 - 2166 WP_000691804 DotA/TraY family protein traY
QUE74_RS29085 (EsCdI10290_05767) 81803..82372 - 570 WP_000650755 conjugal transfer protein TraX -
QUE74_RS29090 (EsCdI10290_05768) 82369..83574 - 1206 WP_001189539 conjugal transfer protein TraW traW
QUE74_RS29095 (EsCdI10290_05769) 83535..84152 - 618 WP_000286861 hypothetical protein traV
QUE74_RS29100 (EsCdI10290_05770) 84152..87196 - 3045 WP_001024760 hypothetical protein traU
QUE74_RS29105 (EsCdI10290_05771) 87421..87966 + 546 WP_001369268 hypothetical protein -
QUE74_RS29110 87939..88319 - 381 WP_000690882 hypothetical protein -
QUE74_RS29115 (EsCdI10290_05772) 88319..88678 - 360 WP_000379509 hypothetical protein -
QUE74_RS29120 (EsCdI10290_05773) 88737..89555 - 819 WP_072148514 conjugal transfer protein TraT traT
QUE74_RS29125 (EsCdI10290_05774) 89470..89721 - 252 WP_001277575 hypothetical protein -
QUE74_RS29130 (EsCdI10290_05775) 89778..90176 - 399 WP_000086067 DUF6750 family protein traR
QUE74_RS29135 (EsCdI10290_05776) 90223..90753 - 531 WP_000987022 conjugal transfer protein TraQ traQ
QUE74_RS29140 (EsCdI10290_05777) 90750..91463 - 714 WP_000787006 conjugal transfer protein TraP traP
QUE74_RS29145 (EsCdI10290_05778) 91460..92791 - 1332 WP_009453278 conjugal transfer protein TraO traO
QUE74_RS29150 (EsCdI10290_05779) 92795..93769 - 975 WP_001165565 DotH/IcmK family type IV secretion protein traN
QUE74_RS29155 (EsCdI10290_05780) 93780..94475 - 696 WP_001287261 DotI/IcmL family type IV secretion protein traM
QUE74_RS29160 (EsCdI10290_05781) 94487..94837 - 351 WP_001056365 hypothetical protein traL
QUE74_RS29165 (EsCdI10290_05782) 94854..98870 - 4017 WP_021554958 LPD7 domain-containing protein -
QUE74_RS29170 (EsCdI10290_05783) 98934..99224 - 291 WP_000817799 hypothetical protein traK
QUE74_RS29175 (EsCdI10290_05784) 99221..100369 - 1149 WP_000625246 plasmid transfer ATPase TraJ virB11
QUE74_RS29180 (EsCdI10290_05785) 100353..101189 - 837 WP_000848138 type IV secretory system conjugative DNA transfer family protein traI
QUE74_RS29185 (EsCdI10290_05786) 101186..101638 - 453 WP_001081198 DotD/TraH family lipoprotein -
QUE74_RS29190 (EsCdI10290_05787) 101687..101857 - 171 WP_000482367 hypothetical protein -
QUE74_RS29195 (EsCdI10290_05788) 101997..103199 - 1203 WP_000980151 conjugal transfer protein TraF -
QUE74_RS29200 (EsCdI10290_05789) 103289..104110 - 822 WP_000804090 hypothetical protein traE
QUE74_RS29205 (EsCdI10290_05790) 104370..104690 + 321 WP_000695052 hypothetical protein -
QUE74_RS29210 (EsCdI10290_05791) 104782..105072 - 291 WP_000681898 hypothetical protein -
QUE74_RS29215 (EsCdI10290_05792) 105114..106283 - 1170 WP_000486835 site-specific integrase -
QUE74_RS29220 (EsCdI10290_05793) 106335..106736 + 402 Protein_122 phage tail protein -
QUE74_RS29225 (EsCdI10290_05794) 107024..108262 - 1239 WP_032072136 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
QUE74_RS29230 (EsCdI10290_05795) 108267..108902 - 636 WP_000478596 prepilin peptidase -
QUE74_RS29235 (EsCdI10290_05796) 108921..109394 - 474 WP_000870986 lytic transglycosylase domain-containing protein virB1
QUE74_RS29240 (EsCdI10290_05797) 109448..109984 - 537 WP_001011156 type 4 pilus major pilin -
QUE74_RS29245 (EsCdI10290_05798) 110058..111158 - 1101 WP_000670135 type II secretion system F family protein -
QUE74_RS29250 (EsCdI10290_05799) 111160..112668 - 1509 WP_000886638 GspE/PulE family protein virB11
QUE74_RS29255 (EsCdI10290_05800) 112753..113205 - 453 WP_001112926 type IV pilus biogenesis protein PilP -
QUE74_RS29260 (EsCdI10290_05801) 113195..114490 - 1296 WP_000129889 type 4b pilus protein PilO2 -
QUE74_RS29265 (EsCdI10290_05802) 114511..116130 - 1620 WP_001034998 PilN family type IVB pilus formation outer membrane protein -
QUE74_RS29270 (EsCdI10290_05803) 116161..116598 - 438 WP_000539685 type IV pilus biogenesis protein PilM -

Host bacterium

ID   13534 GenBank   NZ_AP025743
Plasmid name   10290|pla2 Incompatibility group   IncB/O/K/Z
Plasmid size   123362 bp Coordinate of oriT [Strand]   54007..54079 [-]
Host baterium   Escherichia sp. 10290

Cargo genes

Drug resistance gene   blaTEM-1B, sul2, aph(3'')-Ib, aph(6)-Id, aph(3')-Ia, tet(A)
Virulence gene   -
Metal resistance gene   merE, merD, merA, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -