Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113098
Name   oriT_10290|pla1 in_silico
Organism   Escherichia sp. 10290
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_AP025742 (121243..121366 [-], 124 nt)
oriT length   124 nt
IRs (inverted repeats)      92..99, 113..120  (ATAATGTA..TACATTAT)
 90..95, 107..112  (AAATAA..TTATTT)
 39..46, 49..56  (GCAAAAAC..GTTTTTGC)
 3..10, 15..22  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 124 nt

>oriT_10290|pla1
GGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTGCTATTTGAATCATTAACTTATGTCTTAAATAATGTATTTTAATTTATTTTACATTATAAAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   8407 GenBank   WP_000986971
Name   traI_QUE74_RS27845_10290|pla1 insolico UniProt ID   _
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 192129.86 Da        Isoelectric Point: 5.8508

>WP_000986971.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Escherichia]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGKLPDGADLSRMQ
DGSNRHRPGYDLTFSAPKSVSMMAMPGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIRAAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAESRMQTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTTGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELAMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLISDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKIPVAEGERLRVTGKIPGLRISGGDRLQVASVSEDAMTVVVPGRAEPASLPVGYSPFTALKL
ESGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVVESKNLAFSQVDLLTEAKSFAAEGTSFTDLGREINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETIDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDAIVLVDNVYHRIAGISRDDGLITLQDTDGNSRLISPREAVAEGVT
LYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVMAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVF
EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIALGRKYPQPYVALPAFDRNGRSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSEDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAEEKPDQP
DDKTEQAVRDIAGQERDRAVTSEREAVLPESVLREPQRVQDAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(10-283)

DNA helicase TraI (TraI_C)

(1434-1557)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-626)

AAA domain (AAA_30)

(969-1156)


  Protein structure



No available structure.




Auxiliary protein

ID   4814 GenBank   WP_001254381
Name   WP_001254381_10290|pla1 insolico UniProt ID   _
Length   75 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 75 a.a.        Molecular weight: 9037.10 Da        Isoelectric Point: 9.9851

>WP_001254381.1 MULTISPECIES: conjugal transfer relaxosome protein TraY [Escherichia]
MRRRNAKAGTSRTISVYLDEDTNNRLIRAKDRSGRSKTIEVQIRLRDHLKRYPDFYNEEIFREVTEESES
TFKEL

  Protein domains


Predicted by InterproScan.

TraY domain (TraY)

(14-61)


  Protein structure



No available structure.



ID   4815 GenBank   WP_001151555
Name   WP_001151555_10290|pla1 insolico UniProt ID   _
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14461.36 Da        Isoelectric Point: 4.5221

>WP_001151555.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Escherichia]
MAKVQAYVSDEIVYKINEIVERRRAEGAKSTDVSFSSISTMLLELGLRVYEAQMERKESAFNQAEFNKVL
LECAIKTQSTVAKILGIESLSPHVSGNPKFEYANMVEDIRDKVSSEMERFFPENDEE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-126)


  Protein structure



No available structure.




T4CP

ID   9684 GenBank   WP_000009375
Name   traD_QUE74_RS27860_10290|pla1 insolico UniProt ID   _
Length   714 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 714 a.a.        Molecular weight: 81137.72 Da        Isoelectric Point: 5.1669

>WP_000009375.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Escherichia]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKLSWQTFVNGCIYWWCTTLEGMR
DLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATIVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPRDLNPEMENRLSAVLAAREAEGRQMASLFEPEVVSGEDVTQAEQPQQPVSSVINDK
KSDAGVSVPAGGIAQELKMKPEEEMEQQLPPGISESGEVVDMAVYEAWQREQNPDIQQQMQRREEVNINV
HRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(173-560)

  Protein structure



No available structure.



ID   9685 GenBank   WP_001064271
Name   traC_QUE74_RS27975_10290|pla1 insolico UniProt ID   _
Length   875 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 875 a.a.        Molecular weight: 99434.22 Da        Isoelectric Point: 5.9665

>WP_001064271.1 MULTISPECIES: type IV secretion system protein TraC [Escherichia]
MNNPLEAVTQAVNSLVTALKLPDESSKANEILGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANETIVEALDHMLRTKLPRGIPLCIHLMSSQLVGERIKYGLREFSWSGEQAERFNAITRAYYMKAAE
TLFPLPEGLNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASLQGAYITTQTVDAQAFIDIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAFL
WNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLVKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIVLLDQYT
ANGTYGRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGWR
LLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRQEGL
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(289-446)

TraG P-loop domain (P-loop_TraG)

(468-771)

TraC protein (TraC_F_IV)

(38-276)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 92912..121938

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE74_RS27850 (EsCdI10290_05518) 92278..92505 + 228 WP_000450526 toxin-antitoxin system antitoxin VapB -
QUE74_RS27855 (EsCdI10290_05519) 92505..92903 + 399 WP_000911316 type II toxin-antitoxin system VapC family toxin -
QUE74_RS27860 (EsCdI10290_05520) 92912..95056 - 2145 WP_000009375 type IV conjugative transfer system coupling protein TraD virb4
QUE74_RS27865 (EsCdI10290_05521) 95112..95842 - 731 Protein_112 hypothetical protein -
QUE74_RS27870 (EsCdI10290_05522) 96077..96556 - 480 WP_001355324 hypothetical protein -
QUE74_RS27875 (EsCdI10290_05523) 96760..97491 - 732 WP_001364068 conjugal transfer complement resistance protein TraT -
QUE74_RS27880 (EsCdI10290_05524) 97523..98026 - 504 WP_000605506 hypothetical protein -
QUE74_RS27885 (EsCdI10290_05525) 98042..100885 - 2844 WP_001006995 conjugal transfer mating-pair stabilization protein TraG traG
QUE74_RS27890 (EsCdI10290_05526) 100882..102255 - 1374 WP_001137366 conjugal transfer pilus assembly protein TraH traH
QUE74_RS27895 (EsCdI10290_05527) 102242..102634 - 393 WP_000138366 F-type conjugal transfer protein TrbF -
QUE74_RS27900 (EsCdI10290_05528) 102588..102902 - 315 WP_001244895 P-type conjugative transfer protein TrbJ -
QUE74_RS27905 (EsCdI10290_05529) 102892..103428 - 537 WP_000059854 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
QUE74_RS27910 103415..103699 - 285 WP_000624195 type-F conjugative transfer system pilin chaperone TraQ -
QUE74_RS27915 103780..104115 + 336 WP_000415768 hypothetical protein -
QUE74_RS27920 104096..104401 - 306 WP_236922840 conjugal transfer protein TrbA -
QUE74_RS27925 (EsCdI10290_05531) 104446..105189 - 744 WP_001030399 type-F conjugative transfer system pilin assembly protein TraF traF
QUE74_RS27930 (EsCdI10290_05532) 105182..105439 - 258 WP_000864356 conjugal transfer protein TrbE -
QUE74_RS27935 105453..107357 - 1905 WP_000662057 type-F conjugative transfer system mating-pair stabilization protein TraN traN
QUE74_RS27940 107385..107603 + 219 WP_001364024 hypothetical protein -
QUE74_RS27945 107592..107972 - 381 WP_000009645 hypothetical protein -
QUE74_RS27950 (EsCdI10290_05534) 107969..108607 - 639 WP_000087541 type-F conjugative transfer system pilin assembly protein TrbC trbC
QUE74_RS29505 108634..109153 - 520 Protein_130 hypothetical protein -
QUE74_RS27960 (EsCdI10290_05536) 109429..110421 - 993 WP_001355328 conjugal transfer pilus assembly protein TraU traU
QUE74_RS27965 (EsCdI10290_05537) 110418..111050 - 633 WP_000650119 type-F conjugative transfer system protein TraW traW
QUE74_RS27970 (EsCdI10290_05538) 111047..111433 - 387 WP_000214089 type-F conjugative transfer system protein TrbI -
QUE74_RS27975 (EsCdI10290_05539) 111430..114057 - 2628 WP_001064271 type IV secretion system protein TraC virb4
QUE74_RS27980 (EsCdI10290_05540) 114217..114438 - 222 WP_001278688 conjugal transfer protein TraR -
QUE74_RS27985 (EsCdI10290_05541) 114573..115088 - 516 WP_000802746 type IV conjugative transfer system lipoprotein TraV traV
QUE74_RS27990 (EsCdI10290_05542) 115085..115405 - 321 WP_001057269 DUF2689 domain-containing protein -
QUE74_RS27995 (EsCdI10290_05543) 115392..115979 - 588 WP_000002892 conjugal transfer pilus-stabilizing protein TraP -
QUE74_RS28000 (EsCdI10290_05544) 115969..117399 - 1431 WP_000146619 F-type conjugal transfer pilus assembly protein TraB traB
QUE74_RS28005 (EsCdI10290_05545) 117399..118127 - 729 WP_001355329 type-F conjugative transfer system secretin TraK traK
QUE74_RS28010 (EsCdI10290_05546) 118114..118680 - 567 WP_000399757 type IV conjugative transfer system protein TraE traE
QUE74_RS28015 (EsCdI10290_05547) 118702..119013 - 312 WP_000012121 type IV conjugative transfer system protein TraL traL
QUE74_RS28020 (EsCdI10290_05548) 119028..119387 - 360 WP_000340277 type IV conjugative transfer system pilin TraA -
QUE74_RS28025 (EsCdI10290_05549) 119421..119648 - 228 WP_001254381 conjugal transfer relaxosome protein TraY -
QUE74_RS28030 (EsCdI10290_05550) 119742..120428 - 687 WP_000332479 PAS domain-containing protein -
QUE74_RS28035 (EsCdI10290_05551) 120622..121005 - 384 WP_001151555 conjugal transfer relaxosome DNA-binding protein TraM -
QUE74_RS28040 (EsCdI10290_05552) 121291..121938 + 648 WP_000614283 transglycosylase SLT domain-containing protein virB1
QUE74_RS28045 (EsCdI10290_05553) 122235..123056 - 822 Protein_148 DUF932 domain-containing protein -
QUE74_RS28050 (EsCdI10290_05554) 123175..123462 - 288 WP_000107535 hypothetical protein -
QUE74_RS28055 (EsCdI10290_05555) 123713..124717 + 1005 WP_024166184 IS110 family transposase -
QUE74_RS28060 124811..125071 - 261 WP_000547943 hypothetical protein -
QUE74_RS28065 125141..125313 + 173 Protein_152 hypothetical protein -
QUE74_RS28070 125738..125863 - 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
QUE74_RS28075 (EsCdI10290_05556) 125805..125954 - 150 Protein_154 plasmid maintenance protein Mok -
QUE74_RS28080 125976..126164 + 189 WP_001355331 hypothetical protein -
QUE74_RS28085 (EsCdI10290_05557) 126109..126895 - 787 Protein_156 plasmid SOS inhibition protein A -

Host bacterium

ID   13533 GenBank   NZ_AP025742
Plasmid name   10290|pla1 Incompatibility group   IncFIB
Plasmid size   224339 bp Coordinate of oriT [Strand]   121243..121366 [-]
Host baterium   Escherichia sp. 10290

Cargo genes

Drug resistance gene   -
Virulence gene   hlyC, faeJ, faeI, faeH, faeF, faeE, faeD, faeC, espP
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIF11