Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113091
Name   oriT_pANY103d in_silico
Organism   Aminobacter sp. SS-2016 strain Y103A
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_AP017990 (39819..39863 [+], 45 nt)
oriT length   45 nt
IRs (inverted repeats)      22..27, 32..37  (CGTCGC..GCGACG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 45 nt

>oriT_pANY103d
GGTTCCAAGGGCGCAATTATACGTCGCTGACGCGACGCCCTGCGT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   8402 GenBank   WP_286300137
Name   traA_Amn_RS32365_pANY103d insolico UniProt ID   _
Length   1100 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1100 a.a.        Molecular weight: 123511.06 Da        Isoelectric Point: 9.9531

>WP_286300137.1 Ti-type conjugative transfer relaxase TraA [Aminobacter sp. SS-2016]
MAIAHFSAGILSRGNGRSALLSAAYRHCARMAHEREARTIDYTRKQGLLHEEFVLPVDAPNWARMLIADR
SIAGASETFWNKVEAYEKRSDAQLAKDLTIALPLELTPEQSIALVRDFVEKHILAKGMVADWVYHDNPGN
PHIHLMTTLRPLTEDGFGAKKVPVLGEDGQPLRNKAGKIVYALWAGGTDDFNAFRDAWFERLNHHLALNG
ISLRVDGRSYEKQGIDLVPTIHLGVGAKAIERKAEKQGVRPELERLELNEKRRSENARRILRNPGIVLDL
ITREKSVFDSRDVAKVLNRYVDDPAVFQQLLARIIQSPEVLRLQRDTIDFATGERVPARYTTRELIRIEA
DMALRSVSLAARASHGVSAKVLEATFARHSSLSEEQRTAIEHVAGSARIAAVVGRAGAGKTTMMKAAREA
WELAGYRVVGGALAGKAAEGLEKEAGIVSRTLASWQLRWQQGRDTLDDRCVFVMDEAGMVASKQMAGFVE
AVVRAGAKIVLVGDPEQLQPIEAGAAFRAIVDRIGYAELETIYRQREDWMRHASLDLARGNIEQGLLAYR
TRGHVVGEKLKSEAVESLISAWNHDYDPAKSTLMLAHLRRDVRMLNMMARAKLVERGIVGEGHVFKTADG
IRHFDAGDQIVFLKNEGSLGVKNGMIGRVVEAAPDRIVAVVGEGDQRRQVIVEQRFYNNVDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHREDLQVYFGRRSFEMNGGLTKILSRRNAKETTLDYERGTLY
RQALQFAESRGLHIMQVARTLMHDRLDWTLRQKSKLTDLTTRLRQLGERFGLRQSPPTQPANEAKPMVAG
ITRFENSVADTVEQKLDADLALKKQWQEVSIRFRYVFADPETAFRAMNFDAVLADRDTARQVLQKLETEP
VSIGPLKGKTGILASKTDREVRRVAEVNVPALKRDLERYLQIRESAVQRHEAEENTLRQRVSIDIPALSP
AARAVLERVRDAIDRNDLPAALAYALSNRETKLEIDEFNKAVAQRFGERTVLTNAAREPSSKLFDKLSEG
MQPQEKERLKEAWPVMRTAQQLAAHERTTETLRQVEELRLAQRQAPVVKQ

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(382-566)

MobA/MobL family (MobA_MobL)

(17-243)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(855-951)

Viral (Superfamily 1) RNA helicase (Viral_helicase1)

(692-736)


  Protein structure



No available structure.




T4CP

ID   9677 GenBank   WP_286300094
Name   tcpA_Amn_RS32250_pANY103d insolico UniProt ID   _
Length   1819 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 1819 a.a.        Molecular weight: 199132.71 Da        Isoelectric Point: 5.3808

>WP_286300094.1 FtsK/SpoIIIE domain-containing protein [Aminobacter sp. SS-2016]
MNPSDLIGAAGATSIRLRLDALSPEDGMARYLLDRLTGEQVAAITQALLTDAKATELLNIALPRSLVSPF
GLPDSVMTDERMVAIRHADYDRPALLFANTDEDQGASLGDVTLIGAKQLTEEPGPWVEAAAAGLGLSEGQ
VATWMAALKGLTSADDWTLHQIATYVAMTRTRIETDAVPVTDALGWALSALRLPRDSGYFLGLGEREREV
PRRWKKLFEKLVAERKPLMVKQRPNRQLIENDELREQFAEVRDDIPAEVHSAIEAFIEAAPGWGPQAEAL
SLFEWEAESVLQLFSGIKLKKTSLAQETINFFEFTFPDRLSPADNEYLRALKGRSLKETREDDREFFEAH
RDDLAQDKALRVKWERFVFGRPIECTDFLEGLLRVIERLFGQVNLGGGPRKLSIKSARRSRNQWLDLNAD
VGLYFGLRYRGLPALLGAAVEWDVPHLFSYEELLDRAKARQKKYRRNESTARSALQIKFDVALTVGRERA
TVQLVWTGQPSAIGLELPKDLGRLIKRPFGRSSVARLSVSRKGALQSVSLSDTGTLQPAFGQDAGTLIPR
TSTGIDLAKLFPKLLREAKEAGRITQAGADDIAAAWKQFATMYADALTSLQSSGYASATLIAQADAYGAL
LNSLAKNAIGDLNRRDIWEPVLRIGTIEVLGSAPSAIVAPWHPLRLAGVAAKMRSVTGLADYLLSDVDVN
FGDSRLFFSDLRDELNHPLYPEVAVGYDGSEPVLLTETSTVNDYSLIERPVRDPSEATTDVDPSEAARQI
RALLERYLDLQPHERSNLSIMLYNCDAAGLPLATVSALSSVQDQEEVHCNVLVRHRDRARLSGVYTELLE
RSENDPDAVVVSETSRNFMSKLRIGVMLDVAGGSKSGGAREIDVAFLHDVVSRQAREQWFPVPALTDNPT
LLEHVPARWSYRRVTAEDELKATSYLTCPRQPNAGWAYIDAVANVIRRQSHGPDEHYLPARQISFQDGGL
KGMFDEVHKLAEWVATYDDLLDKRQLAAQGINVIRYRRQRTHGRNMVVSSTSELRILHVLFKRRLAELSL
GLGDDRLAGLARRMIEDANAISGDIVLRAAKRGVSAGELIGLVLSRALVEQELGERAAVAWFLLDDYAEW
LGQKEEGIADILGISLSTGEDGLPKLRAIVTEAKYVDQSGLAEASRKSRSQLRQTVARIDDALFGDPGRL
DRDLWLSRISDLLLDGTAALGQANLLERVRDGIRRGAVPIDLRGYSHIFVSGPATDGISPGEQDELPEIQ
MGLQETFTREGLRKLIKAYEAKQSLTELRGQLGEKHTWNSVSYRHPAPRVDWTKTIKEEHSIAGSEKPIS
HDYDQDDDEGDPEGGIVIEAVEPGTNGNGSVAVSGDGGGLSGGEITPAPAPNQTKDQPTISLPSLTNTQP
SASALPSGSTAAPTPDKFPRAGAEKGIAALIEARAQKARENSADEREWLDSTAQKLRSALLGYNLQAKIV
GTRLTPNAALIRFMGSDRLRVEDIEAKQSSLLTTHGLRLISISPLPGEIVVGVARPQRQVVSLWDVWGRR
EINRNAAGVNTSFVLGLKELDGDILYLNLGVPFAGGQQHEPHTLVAGATGSGKSVLIQALLLDIAATNSS
DLAHIHLIDPKMGVDYAAIERLPHLQGGVIVDQTRAVVVMEGLVTEMERRYELFRSKGARDIRAFNSKVD
TAERLPYVFLVHDEFAEWMLTEDYKSAVTSNVSRLGVKARAAGMHLIFAAQRPDANVMPMQLRDNLGNRL
ILKVASVGTSEIALGVKGAEQLLGLGHLAARLSGEPAIIYAQAPFLSDDDIDAAVDAILASDAVSGPAD

  Protein domains


Predicted by InterproScan.

FtsK alpha domain (FtsK_alpha)

(1439-1526)

FtsK/SpoIIIE family (FtsK_SpoIIIE)

(1578-1683)

  Protein structure



No available structure.



ID   9678 GenBank   WP_286300145
Name   traG_Amn_RS32350_pANY103d insolico UniProt ID   _
Length   639 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 639 a.a.        Molecular weight: 68964.22 Da        Isoelectric Point: 9.3643

>WP_286300145.1 Ti-type conjugative transfer system protein TraG [Aminobacter sp. SS-2016]
MNRLLLTILPAALMITTMLAMLGIETWLSGFGTSPQAKLMLGRIGLALPYSAAAAIGMMALFATAGAANI
KTAGLSVLAGASFVCIAAIAREGWRLRALADQVPHGQSVFAYTDPSSMLGAFAAVLGGIFALRVAIRGNA
AFAKAAPRRIIGKRAVHGDADWMKMADAVKLFPDAGGIVIGERYRVDKDSVTAIPFRAGEALSWGAGGKS
PLLCFDGSFGSSHGIVFAGSGGFKTTSVTIPTALKWGGGLVVLDPSSEVAPMVVDHRRKAGRNVIVLDPA
TPAIGFNALDWIGRHGNTKEEDIVAVATWIMTDNARTASARDDFFRASAMQLLTALIADVCLSVHTDEKN
QTLRRVRANLSEPEPKLRERLTRIYEQSESDFVKENVAVFVNMTPETFSGVYANAVKETHWLSYPNYAAL
VSGDSFSTDDLAEGGADLFIALDLKVLEAHPGLARVVIGSLLNAIYNRNGNVEGRTLFLLDEVARLGYLR
ILETARDAGRKYGITLAMIFQSIGQMREAYGGRDATNKWFESASWISFAAINDSDTADYISKRCGDTTVE
VDQTNRSTGMKGSSRSRSKQLSRRPLIMPHEVLRMRSDEQIVFTAGNAPLRCGRAMWFRRDDMKACVKPN
VFFRESGGG

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(161-631)

  Protein structure



No available structure.



ID   9679 GenBank   WP_286300047
Name   t4cp2_Amn_RS32535_pANY103d insolico UniProt ID   _
Length   822 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 822 a.a.        Molecular weight: 91789.57 Da        Isoelectric Point: 5.7828

>WP_286300047.1 conjugal transfer protein TrbE [Aminobacter sp. SS-2016]
MVALKTFRHSEPSFADLVPYAGLVDNGVILLKDGSLMAGWYFAGPDSESSTDAERNEVSRQINAILSRLG
SGWMIQVEAIRVPTGDYPAEDDCHFPDPVTRAIDAERRAHFQKERGHFESRHALILTWRPPEPRRSGLTR
YVYSDTASRSASYADKALETFLTSIREVEQYLANVVSIRRMMTRETSERGGFRVARYDELFQFIRFCITG
ENHPVRLPDIPMYLDWLVTAELQHGLTPLVENRFLGVVAIDGLPAESSPGILNSLDLMPLTYRWSSRFIF
FDAEEARAKLERTRKKWQQKVRPFFDQLFQTQSRSVDQDAMMMVAETEDAIAEASSQLVAYGYYTPVIVI
FDEQQARCQEKCEAVRRLIQAEGFGARIETLNATDAFLGSLPGVSYANIREPLINTRNLADLIPLNSVWS
GSPTAPCPFYPPGSPPLMQVASGSTPFRLNLHVDDVGHTLIFGPTGSGKSTLLALVAAQFRRYSQAQVFA
FDKGGSMLPLTLGLNGNHYQIGGDIGPSAEGEVKALAFCPLAELSTDGDRAWASEWIETLVALQGVTITP
DYRNAISRQIGLMAESRGRSLSDFVSGVQMREIKDALHHYTVDGTMGQLLDAEEDGLALSAFQCFEIEEL
MNMGERNLVPVLTYLFRRIEKRLTGTPSLIILDEAWLMLGHPVFRDKIREWLKVLRKANCAVVLATQSIS
DAERSGIIDVLKESCPTKICLPNGAAREPGTREFYERIGFNERQIEIVATALPKREYYVVSPDGRRLFDM
ALGPVALSFVGASGKEDLKRIRALHSEHGAGWPLHWLQQRGIAHAETLFPAQ

  Protein domains


Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(186-400)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 69458..79159

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Amn_RS32480 (Amn_pd00580) 65281..66255 + 975 WP_286300017 tripartite tricarboxylate transporter substrate binding protein -
Amn_RS32485 (Amn_pd00590) 66341..66796 + 456 WP_286300019 tripartite tricarboxylate transporter TctB family protein -
Amn_RS32490 (Amn_pd00600) 66827..68356 + 1530 WP_286300021 tripartite tricarboxylate transporter permease -
Amn_RS32705 68505..68576 - 72 WP_353622459 hypothetical protein -
Amn_RS32495 (Amn_pd00610) 68800..69081 + 282 Protein_60 glutathione S-transferase family protein -
Amn_RS32500 (Amn_pd00620) 69458..70774 - 1317 WP_286300022 IncP-type conjugal transfer protein TrbI virB10
Amn_RS32505 (Amn_pd00630) 70786..71259 - 474 WP_286300025 conjugal transfer protein TrbH -
Amn_RS32510 (Amn_pd00640) 71259..72113 - 855 WP_286300028 P-type conjugative transfer protein TrbG virB9
Amn_RS32515 (Amn_pd00650) 72131..72808 - 678 WP_286300032 conjugal transfer protein TrbF virB8
Amn_RS32520 (Amn_pd00660) 72826..74022 - 1197 WP_286300035 P-type conjugative transfer protein TrbL virB6
Amn_RS32525 (Amn_pd00670) 74016..74246 - 231 WP_286300041 entry exclusion protein TrbK -
Amn_RS32530 (Amn_pd00680) 74243..75052 - 810 WP_286300044 P-type conjugative transfer protein TrbJ virB5
Amn_RS32535 (Amn_pd00690) 75024..77492 - 2469 WP_286300047 conjugal transfer protein TrbE virb4
Amn_RS32540 (Amn_pd00700) 77502..77801 - 300 WP_286300050 conjugal transfer protein TrbD virB3
Amn_RS32545 (Amn_pd00710) 77794..78198 - 405 WP_286300052 conjugal transfer pilin TrbC virB2
Amn_RS32550 (Amn_pd00720) 78188..79159 - 972 WP_286300054 P-type conjugative transfer ATPase TrbB virB11
Amn_RS32555 (Amn_pd00730) 79156..79791 - 636 WP_286300060 acyl-homoserine-lactone synthase TraI -

Host bacterium

ID   13526 GenBank   NZ_AP017990
Plasmid name   pANY103d Incompatibility group   -
Plasmid size   80166 bp Coordinate of oriT [Strand]   39819..39863 [+]
Host baterium   Aminobacter sp. SS-2016 strain Y103A

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -