Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 113069
• Name: oriT_pSZ.NG02
• Organism: Staphylococcus epidermidis strain NG02
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP127870 (5348..5468 [-], 121 nt)
• oriT length: 121 nt
• IRs (inverted repeats): 53..58, 64..69 (TTGGGG..CCCCAA) 24..30, 35..41 (TTTTTGC..GCAAAAA) 1..8, 14..21 (AGTGGCTA..TAGCCACT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 121 nt

>oriT_pSZ.NG02
AGTGGCTAGCAATTAGCCACTCTTTTTTGCGTAAGCAAAAATCATAAGGGGTTTGGGGATGTGCCCCAACAAGCTGGCGTCTCTACCACGTTAGTGGCTAGCAAAGCCAATGCTTGCCAAA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_QQ988_RS12030_pSZ.NG02

• ID: 8387
• GenBank: WP_002485550
• Name: Relaxase_QQ988_RS12030_pSZ.NG02
• UniProt ID: A0A9P2Z0A8
• Length: 336 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 336 a.a.
• Molecular weight: 38534.32 Da
• Isoelectric Point: 7.1083

>WP_002485550.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Bacilli]
MATTKISATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKASRELYGKTDGNQGHVIIQSFKPGEVTPE
QCNQLGLALAEKLAPNHQVAVYTHNDTDHVHNHIVMNSIDLETGKKFNNNKQALRNVRDFNDEVCLEHGL
SVPDKDTARLRYTQTEKAIADPNTKSTAQYSWKDEIREAIDQSQATNMDEFKDHLNQHGIEIERVTPKSI
TYRHLTEDKKVRGRKLGEDYNKGGIEDGFERQIQRRRQQERDSDTEFQPKRPTSNRDEATERDTGVTQSD
WDQFAQDTNELERSRQAAESARLAHEKARRDREERARAKQASRRIINNDRDHGLEL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (9-242)

Protein structure

No available structure.

Host bacterium

• ID: 13504
• GenBank: NZ_CP127870
• Plasmid name: pSZ.NG02
• Incompatibility group: -
• Plasmid size: 7956 bp
• Coordinate of oriT [Strand]: 5348..5468 [-]
• Host baterium: Staphylococcus epidermidis strain NG02

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -