Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 113059
• Name: oriT_HRBS-10TDI13|unnamed
• Organism: Bacillus subtilis strain HRBS-10TDI13
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP015223 (738..760 [-], 23 nt)
• oriT length: 23 nt
• IRs (inverted repeats): 2..7, 17..22 (CCCCCC..GGGGGG) 1..6, 17..22 (CCCCCC..GGGGGG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 23 nt

>oriT_HRBS-10TDI13|unnamed
CCCCCCCACGCTAACAGGGGGGT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_A4A60_RS22210_HRBS-10TDI13|unnamed

• ID: 8381
• GenBank: WP_048407173
• Name: mobV_A4A60_RS22210_HRBS-10TDI13|unnamed
• UniProt ID: _
• Length: 482 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 482 a.a.
• Molecular weight: 56667.44 Da
• Isoelectric Point: 9.6717

>WP_048407173.1 MULTISPECIES: MobV family relaxase [Bacillus]
MANYAVIRMEKYKKDRLNGTQKHNQREFQKSKNENIDRERTHLNYDLVNEKPISYSKAIHEKIEGRVKRK
VRADAVLVSEFLITASPDYMNGLSDEEQRRYFETAVDHLKEKYSAENMLYATVHMDEATPHMHVGIVPIT
EDGRLSAKDFFNGKLKMKAIQDDFHRHMVENGFDLVRGEPSEKKHENVHQYKINQRKAELERLNAEIALK
EKQREELEKQNKAVQAVIEVKKESLTVKAEELKMPTIEHEKAWLKKDKVIVPERELHALYAYAEQKTKTA
AELAGQLKSETKEKERWQSIARQEADRADEKDQRLQELQSRIHSEVEASKKEMRRKLAKEFTEEQRQDLR
QEVKEELTTLRTENEELSAENKVLIIQRNSEAAESLKLKQELDKRNGQYAEVLSFAKKQNQTLEKVAGEN
KALKKENKTLKERVAVLEQWKDKMVQWAKEKLPKMRKLAASFFRTAGMPREANKYKDNELER

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-192)

Protein structure

No available structure.

Host bacterium

• ID: 13494
• GenBank: NZ_CP015223
• Plasmid name: HRBS-10TDI13|unnamed
• Incompatibility group: Col
• Plasmid size: 9149 bp
• Coordinate of oriT [Strand]: 738..760 [-]
• Host baterium: Bacillus subtilis strain HRBS-10TDI13

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -