Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113032
Name   oriT_pST34VN3 in_silico
Organism   Salmonella enterica subsp. enterica serovar Typhimurium var. monophasic 4[5]12:i:- strain 02_1644
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OQ658821 (192840..193093 [-], 254 nt)
oriT length   254 nt
IRs (inverted repeats)      152..157, 159..164  (AAAAGT..ACTTTT)
 121..129, 134..142  (TTAAGGCTT..AAGCCTTAA)
 50..55, 59..64  (AATTTT..AAAATT)
Location of nic site      77..78
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 254 nt

>oriT_pST34VN3
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGAAGAAATTTTGATAAAATTCTGGTCAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   8368 GenBank   WP_001011151
Name   mobH_QTM66_RS01045_pST34VN3 insolico UniProt ID   A0A1P8VU63
Length   1048 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1048 a.a.        Molecular weight: 117773.04 Da        Isoelectric Point: 4.6054

>WP_001011151.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPTGI
QTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGGLL
SHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWTPS
SQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYTDG
NDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGEVY
LNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFRNG
IGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDAITKITDTVE
TAVLKVNDLGRSTASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESEDE
SAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDATMALLPGLDEKAFSEEPYFQLTFREGSLD
GMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLELP
PPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEEEDEEHEIIDYTDFTQLSVSRPEVGSC
ATSSSVHNEKLLPEPSELPELNREQNADPQGTIERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAAAT
SVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPASPV
SGHNLNSKVFATTESDQNGEFSLISEGDVTELEFVEIASVLHQILSKMEVAFKRKRKNRFMVSTPNTLYL
TQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(106-302)


  Protein structure


Source ID Structure
AlphaFold DB A0A1P8VU63


T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 22130..31337

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QTM66_RS00080 (pVN3_00075) 17239..17955 - 717 WP_000838218 zinc finger-like domain-containing protein -
QTM66_RS00085 (pVN3_00080) 17952..18152 - 201 WP_000594931 hypothetical protein -
QTM66_RS00090 (pVN3_00085) 18170..19225 - 1056 WP_065311103 hypothetical protein -
QTM66_RS00095 (pVN3_00090) 19702..20577 + 876 WP_000594612 RepB family plasmid replication initiator protein -
QTM66_RS00100 (pVN3_00100) 21726..22079 + 354 WP_000423602 hypothetical protein -
QTM66_RS00105 (pVN3_00105) 22130..22447 + 318 WP_000043356 type IV conjugative transfer system protein TraL traL
QTM66_RS00110 (pVN3_00110) 22459..23247 + 789 WP_000783153 TraE/TraK family type IV conjugative transfer system protein traE
QTM66_RS00115 (pVN3_00115) 23247..24518 + 1272 WP_000592090 type-F conjugative transfer system secretin TraK traK
QTM66_RS00120 (pVN3_00120) 24520..24981 + 462 WP_000521240 plasmid transfer protein HtdO -
QTM66_RS00125 (pVN3_00125) 24971..26326 + 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
QTM66_RS00130 (pVN3_00130) 26334..26816 + 483 WP_065311104 transcriptional regulator -
QTM66_RS00135 (pVN3_00135) 26935..27687 + 753 WP_183079335 protein-disulfide isomerase HtdT -
QTM66_RS00140 (pVN3_00140) 27697..28647 + 951 WP_001022587 IncHI-type conjugal transfer lipoprotein TrhV traV
QTM66_RS00145 (pVN3_00145) 28656..31337 + 2682 WP_000387412 TraC family protein virb4

Host bacterium

ID   13467 GenBank   NZ_OQ658821
Plasmid name   pST34VN3 Incompatibility group   IncHI2
Plasmid size   247148 bp Coordinate of oriT [Strand]   192840..193093 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Typhimurium var. monophasic 4[5]12:i:- strain 02_1644

Cargo genes

Drug resistance gene   aph(3')-Ia, floR, catA2, dfrA14, sul2, aph(3'')-Ib, aph(6)-Id, blaTEM-1B, aac(3)-IId, lnu(F), ant(3'')-Ia, qnrS1, mph(A), ARR-3
Virulence gene   -
Metal resistance gene   terW, terZ, terA, terB, terC, terD, terE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -