Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113029
Name   oriT_p10907_1 in_silico
Organism   Salmonella enterica subsp. enterica serovar Typhimurium var. monophasic 4[5]12:i:- strain 01_0907
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OQ658823 (129901..130185 [-], 285 nt)
oriT length   285 nt
IRs (inverted repeats)      184..189, 191..196  (AAAAGT..ACTTTT)
Location of nic site      109..110
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 285 nt

>oriT_p10907_1
GGTTATTGCTACTTAATGCCGATAACGACTCAGGCTTTGAGGTTTTTTTATACGGTTCACATTTCGTTAGCAAGGTCAGGGTTTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGGGTAATGGCTGAAAGGTTAGTTTTAAGGTTCAAAGCGGCAGTATTAAAATTCCAAAAGTTACTTTTCATCCTTCAGAATCCAGACCTTAATTTCATGTAGAAGATTCGTACAATTGTATTGGCGCAAGGACAATCCGCACATGTCAGAATCAGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   8365 GenBank   WP_001011149
Name   mobH_QTM62_RS00645_p10907_1 insolico UniProt ID   A0A0G3BA46
Length   1011 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1011 a.a.        Molecular weight: 113023.00 Da        Isoelectric Point: 4.5108

>WP_001011149.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MNFRALFLSMQRVFGIFSRRENDVSELMMKDAANFSPFAQIIGEQKYTVPDHPNPEVLKFIEYPTRPAGI
QTFNEQSILSLYRDKLHSISMMLAISDGDIREDAYTFTNLVLKPLIEYIRWIHLLPASENHHHNGIGGLL
SHSLEVAMISLKNANHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLSETLTWAPS
SQSLLDWARENNVVEYEIHWRKRIHNQHNIWSSVFLERILDPVCMSFLDRVKKERVYAKMVTALNVYNDG
NDFLSKCVRTSDYYSTGTDLNVLRDPIMGLRSNDAAARAIGTIKHNFTSININNYKTKPMHIIIVNGEVY
LNENAFLDFVLSDFAAHKFNFPQGDAGKTVLVESLVQRGYVEPYDDERVVHYFIPGTYSENEIASIFRNG
IGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRESVTRVTDTVN
DAIENTPQYGLQLVNGPDADSNNIISSENTESITDSLEESGADISNEIFETQVVTAIDTAETVNADEPEQ
VEEHDDRSQIHLVEQLHEMLLSAPLPHHAVINIDSVPYLDLDAAIALIPGIDEAAFCNGPFFQLTYRDGS
LDGMWIVRDVNNLRLIQLGDNCAGMQVSTSEPRNTSSLKSLFDTSMYQPLDIPEAPSVNEAASPPQTPLE
LPQPRLNAPVAEEASSVAEQTNAHSEPDSVIATEYEQYGHLLEETLDSDGEAYSDLIASDSTEAEYPATD
PQSSDFAQLPRETALSVAPGDLDYSEGAIKPPAPDATGKETILTSPEPAEDVRETVAAVEKASHLSPALA
RLFAVSTHAEKKHEKTQEPSPVKEVKNPTSSTTVKAPISIEPPGAEEKEAVEEFTLLNDGEVTELEYVEI
ATMLHQILTKLSGSFKRKRKNRFMVLTQNTFYLTQSCIEKYGTQLNAPELFNQLPQYQVTSGAVVNTKCI
AFNIPTLVAASDRAKVDIELIINKLKEVGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(106-301)


  Protein structure


Source ID Structure
AlphaFold DB A0A0G3BA46


T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 189719..198887

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QTM62_RS00975 (p01_01050) 185256..185456 - 201 WP_000357613 hypothetical protein -
QTM62_RS00980 (p01_01055) 185474..186550 - 1077 WP_001015069 hypothetical protein -
QTM62_RS00985 (p01_01060) 187085..187960 + 876 WP_001282731 RepB family plasmid replication initiator protein -
QTM62_RS00990 (p01_01065) 188407..188832 - 426 WP_154166887 hypothetical protein -
QTM62_RS00995 (p01_01070) 189314..189667 + 354 WP_038988950 hypothetical protein -
QTM62_RS01000 (p01_01075) 189719..190036 + 318 WP_000203871 type IV conjugative transfer system protein TraL traL
QTM62_RS01005 (p01_01080) 190048..190833 + 786 WP_000771920 TraE/TraK family type IV conjugative transfer system protein traE
QTM62_RS01010 (p01_01085) 190878..192068 + 1191 WP_225374337 type-F conjugative transfer system secretin TraK traK
QTM62_RS01015 (p01_01090) 192070..192510 + 441 WP_001224093 plasmid transfer protein HtdO -
QTM62_RS01020 (p01_01095) 192500..193858 + 1359 WP_000351840 IncHI-type conjugal transfer protein TrhB traB
QTM62_RS01025 (p01_01100) 193867..194379 + 513 WP_000429933 hypothetical protein -
QTM62_RS01030 (p01_01105) 194485..195237 + 753 WP_010892304 protein-disulfide isomerase HtdT -
QTM62_RS01035 (p01_01110) 195247..196197 + 951 WP_001022266 TraV family lipoprotein traV
QTM62_RS01040 (p01_01115) 196206..198887 + 2682 WP_001255540 IncHI-type conjugal transfer ATPase TrhC virb4
QTM62_RS01045 199587..199985 + 399 WP_071925125 hypothetical protein -
QTM62_RS01050 200663..200836 + 174 WP_005046574 hypothetical protein -
QTM62_RS01055 200974..201156 + 183 WP_071925124 hypothetical protein -
QTM62_RS01060 201187..201420 + 234 WP_032489575 hypothetical protein -

Host bacterium

ID   13464 GenBank   NZ_OQ658823
Plasmid name   p10907_1 Incompatibility group   IncFIA
Plasmid size   203427 bp Coordinate of oriT [Strand]   129901..130185 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Typhimurium var. monophasic 4[5]12:i:- strain 01_0907

Cargo genes

Drug resistance gene   qnrS1, blaTEM-176, ant(3'')-Ia, cmlA1, aadA2, dfrA12, floR, sul2, mcr-1.1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -