Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 112974
• Name: oriT_pUAMSEL4
• Organism: Enterococcus faecalis strain UAMS_EL53
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP076502 (5234..5274 [+], 41 nt)
• oriT length: 41 nt
• IRs (inverted repeats): 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 41 nt

>oriT_pUAMSEL4
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_KPU58_RS16255_pUAMSEL4

• ID: 8328
• GenBank: WP_015382137
• Name: mobV_KPU58_RS16255_pUAMSEL4
• UniProt ID: L8BAH8
• Length: 409 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 409 a.a.
• Molecular weight: 48447.75 Da
• Isoelectric Point: 7.0106

>WP_015382137.1 MULTISPECIES: MobV family relaxase [Bacilli]
MSMIVARMQKMKAENLVGIGNHNQRKTKNHSNPDIDTSLSKLNYDLVDRTQNYKTDIENFINENKTTTRA
VRKDAVLVNEWIISSDKDFFDNLTESEIENFFERSKDYFAEKFGEKNIRYATVHLDESTPHMHMGIVPFD
KDNKLSAKRVFNRQALRDVQEELPKYLQDFQFEIERGQKGSERKNLTVPEFKKLKEEEREIKKELEIKKD
ELMAYTKENKIDKEIDITPIKEMEDVEIETDEKSLFGITKTKTVRQWTGNIVLSEKDYLKLRQKVNKGKQ
AEGKLEAILETDVYQENKELKNELKDQIDKNDKDIDDYNDLVKRYNNLYEENTSLKNQIGDLKEEIKLIY
QSTKRFFKDRISDFKAFKEVFKELADSISNISREKGLDSSFKKEFDRENKKKRTKGMSR

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

• AlphaFold DB: L8BAH8

Host bacterium

• ID: 13409
• GenBank: NZ_CP076502
• Plasmid name: pUAMSEL4
• Incompatibility group: -
• Plasmid size: 33652 bp
• Coordinate of oriT [Strand]: 5234..5274 [+]
• Host baterium: Enterococcus faecalis strain UAMS_EL53

Cargo genes

• Drug resistance gene: aadD, VanHAX
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -