Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   112974
Name   oriT_pUAMSEL4 in_silico
Organism   Enterococcus faecalis strain UAMS_EL53
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP076502 (5234..5274 [+], 41 nt)
oriT length   41 nt
IRs (inverted repeats)      14..22, 29..37  (TAAAGTATA..TATACTTTA)
 2..8, 13..19  (ACTTTAT..ATAAAGT)
Location of nic site      27..28
Conserved sequence flanking the
  nic site  
 
 GTGTGTTATA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 41 nt

>oriT_pUAMSEL4
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTACATG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   8328 GenBank   WP_015382137
Name   mobV_KPU58_RS16255_pUAMSEL4 insolico UniProt ID   L8BAH8
Length   409 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 409 a.a.        Molecular weight: 48447.75 Da        Isoelectric Point: 7.0106

>WP_015382137.1 MULTISPECIES: MobV family relaxase [Bacilli]
MSMIVARMQKMKAENLVGIGNHNQRKTKNHSNPDIDTSLSKLNYDLVDRTQNYKTDIENFINENKTTTRA
VRKDAVLVNEWIISSDKDFFDNLTESEIENFFERSKDYFAEKFGEKNIRYATVHLDESTPHMHMGIVPFD
KDNKLSAKRVFNRQALRDVQEELPKYLQDFQFEIERGQKGSERKNLTVPEFKKLKEEEREIKKELEIKKD
ELMAYTKENKIDKEIDITPIKEMEDVEIETDEKSLFGITKTKTVRQWTGNIVLSEKDYLKLRQKVNKGKQ
AEGKLEAILETDVYQENKELKNELKDQIDKNDKDIDDYNDLVKRYNNLYEENTSLKNQIGDLKEEIKLIY
QSTKRFFKDRISDFKAFKEVFKELADSISNISREKGLDSSFKKEFDRENKKKRTKGMSR

  Protein domains


Predicted by InterproScan.

(1-193)


  Protein structure


Source ID Structure
AlphaFold DB L8BAH8


Host bacterium


ID   13409 GenBank   NZ_CP076502
Plasmid name   pUAMSEL4 Incompatibility group   -
Plasmid size   33652 bp Coordinate of oriT [Strand]   5234..5274 [+]
Host baterium   Enterococcus faecalis strain UAMS_EL53

Cargo genes


Drug resistance gene   aadD, VanHAX
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -