Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 112973 |
| Name | oriT_pUAMSEL3  |
| Organism | Enterococcus faecalis strain UAMS_EL53 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP076501 (2947..2996 [-], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pUAMSEL3
AATATCGCAACATGCTAGCATGTTGCTCTGCTCGCAAAAAGAAAGCCTAC
AATATCGCAACATGCTAGCATGTTGCTCTGCTCGCAAAAAGAAAGCCTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 8327 | GenBank | WP_002365873 |
| Name | Relaxase_KPU58_RS15960_pUAMSEL3 |
UniProt ID | _ |
| Length | 561 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 561 a.a. Molecular weight: 65469.22 Da Isoelectric Point: 6.0174
>WP_002365873.1 relaxase/mobilization nuclease domain-containing protein [Enterococcus faecalis]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKRVFEQISDKIAAKEGAKIIEKSPKNS
HKKYTMWETESLYKEKIKSRLDFLLEQSSSINDFLEKAEALNLSVDFSKKWTTYRLLDEPQIKNTRSRSL
SKNDPTRYNYEKIVERLKENKSVLTLKEVVEKYVEKSEKTKNEFDYQLTIDEWQISHKTTRGYYVNVDFG
FGERGKVFIGAYKVDPLENGQYNIYVKRKDFFYLMNEKNADRNRYMTGETLIKQLRLYNGQTPLKKEPVM
RTIDELVNAINFLAANEIEDTRQLELLEEKLEAAFLEAEETLETLDEKMLELHQLSNLLLENELNGELEL
IQQKLKTLLPDATLAEFSYEDVRGEIEAIKTSQSLLENKLERTRNEINQLHEIQAVQKKETANQNNVKPK
L
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKRVFEQISDKIAAKEGAKIIEKSPKNS
HKKYTMWETESLYKEKIKSRLDFLLEQSSSINDFLEKAEALNLSVDFSKKWTTYRLLDEPQIKNTRSRSL
SKNDPTRYNYEKIVERLKENKSVLTLKEVVEKYVEKSEKTKNEFDYQLTIDEWQISHKTTRGYYVNVDFG
FGERGKVFIGAYKVDPLENGQYNIYVKRKDFFYLMNEKNADRNRYMTGETLIKQLRLYNGQTPLKKEPVM
RTIDELVNAINFLAANEIEDTRQLELLEEKLEAAFLEAEETLETLDEKMLELHQLSNLLLENELNGELEL
IQQKLKTLLPDATLAEFSYEDVRGEIEAIKTSQSLLENKLERTRNEINQLHEIQAVQKKETANQNNVKPK
L
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 4755 | GenBank | WP_002365872 |
| Name | WP_002365872_pUAMSEL3 |
UniProt ID | _ |
| Length | 118 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 118 a.a. Molecular weight: 13988.05 Da Isoelectric Point: 9.6548
>WP_002365872.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Enterococcus]
MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVHSLNALVQSELNKLIKRKEQS
MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVHSLNALVQSELNKLIKRKEQS
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 9649 | GenBank | WP_242140990 |
| Name | t4cp2_KPU58_RS15980_pUAMSEL3 |
UniProt ID | _ |
| Length | 609 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 609 a.a. Molecular weight: 70059.14 Da Isoelectric Point: 6.4636
>WP_242140990.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFHRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNVAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEVKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRALEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFHRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNVAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEVKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRALEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 5641..22930
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KPU58_RS15960 (KPU58_15875) | 838..2523 | - | 1686 | WP_002365873 | relaxase/mobilization nuclease domain-containing protein | - |
| KPU58_RS15965 (KPU58_15880) | 2524..2880 | - | 357 | WP_002365872 | plasmid mobilization relaxosome protein MobC | - |
| KPU58_RS15970 (KPU58_15885) | 3126..3398 | - | 273 | WP_002362409 | LtrD | - |
| KPU58_RS15975 (KPU58_15890) | 3431..5590 | - | 2160 | WP_002365871 | ArdC-like ssDNA-binding domain-containing protein | - |
| KPU58_RS15980 (KPU58_15895) | 5641..7470 | - | 1830 | WP_242140990 | VirD4-like conjugal transfer protein, CD1115 family | virb4 |
| KPU58_RS15985 (KPU58_15900) | 7470..7955 | - | 486 | WP_016616031 | PcfB family protein | gbs1365 |
| KPU58_RS15990 (KPU58_15905) | 8116..8724 | - | 609 | WP_242140987 | PcfA protein | - |
| KPU58_RS15995 (KPU58_15910) | 8708..8953 | - | 246 | WP_002382804 | hypothetical protein | - |
| KPU58_RS16000 (KPU58_15915) | 8940..9566 | - | 627 | WP_242140985 | hypothetical protein | prgL |
| KPU58_RS16005 (KPU58_15920) | 9590..12205 | - | 2616 | WP_002365864 | phage tail tip lysozyme | prgK |
| KPU58_RS16010 (KPU58_15925) | 12217..14607 | - | 2391 | WP_002365863 | VirB4-like conjugal transfer ATPase, CD1110 family | virb4 |
| KPU58_RS16015 (KPU58_15930) | 14561..14914 | - | 354 | WP_002365862 | PrgI family mobile element protein | prgIc |
| KPU58_RS16020 (KPU58_15935) | 14916..15716 | - | 801 | WP_002365861 | PrgH | prgHb |
| KPU58_RS16025 (KPU58_15940) | 15731..16027 | - | 297 | WP_002365860 | hypothetical protein | - |
| KPU58_RS16030 (KPU58_15945) | 16041..16268 | - | 228 | WP_002365858 | hypothetical protein | - |
| KPU58_RS16035 (KPU58_15950) | 16315..16749 | - | 435 | WP_002365857 | hypothetical protein | - |
| KPU58_RS16040 (KPU58_15955) | 16769..17668 | - | 900 | WP_025186515 | hypothetical protein | - |
| KPU58_RS16045 (KPU58_15960) | 17710..18567 | - | 858 | WP_002401687 | pheromone response LPXTG-anchored adhesin PrgC | prgC |
| KPU58_RS16050 (KPU58_15965) | 18595..18951 | - | 357 | WP_002365854 | pheromone response system RNA-binding regulator PrgU | - |
| KPU58_RS16055 (KPU58_15970) | 19013..22930 | - | 3918 | WP_242191337 | LPXTG-anchored aggregation substance | prgB |
| KPU58_RS16060 (KPU58_15975) | 23123..25801 | - | 2679 | WP_173950833 | surface exclusion protein SEA1/PrgA | - |
| KPU58_RS16065 (KPU58_15980) | 25812..26117 | - | 306 | WP_010715949 | hypothetical protein | - |
| KPU58_RS16070 | 26419..26640 | - | 222 | WP_016616022 | hypothetical protein | - |
| KPU58_RS16075 (KPU58_15985) | 26691..27071 | - | 381 | WP_002365885 | PrgR | - |
Host bacterium
| ID | 13408 | GenBank | NZ_CP076501 |
| Plasmid name | pUAMSEL3 | Incompatibility group | - |
| Plasmid size | 53420 bp | Coordinate of oriT [Strand] | 2947..2996 [-] |
| Host baterium | Enterococcus faecalis strain UAMS_EL53 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | prgB/asc10 |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |