Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 112898
• Name: oriT_pUAMSEL3
• Organism: Enterococcus faecalis strain UAMS_EL54
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP076496 (19439..19488 [+], 50 nt)
• oriT length: 50 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 50 nt

>oriT_pUAMSEL3
AATATCGCAACATGCTAGCATGTTGCTCTGCTCGCAAAAAGAAAGCCTAC

oriT secondary structure

Predicted by RNAfold.

T4CP

t4cp2_KPU55_RS16050_pUAMSEL3

• ID: 9622
• GenBank: WP_242140990
• Name: t4cp2_KPU55_RS16050_pUAMSEL3
• UniProt ID: _
• Length: 609 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 609 a.a.
• Molecular weight: 70059.14 Da
• Isoelectric Point: 6.4636

>WP_242140990.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFHRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNVAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEVKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRALEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (144-558)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 3868..16794

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPU55_RS15980 (KPU55_15880)	3484..3840	+	357	WP_002365854	pheromone response system RNA-binding regulator PrgU	-
KPU55_RS15985 (KPU55_15885)	3868..4725	+	858	WP_002401687	pheromone response LPXTG-anchored adhesin PrgC	prgC
KPU55_RS15990 (KPU55_15890)	4767..5666	+	900	WP_025186515	hypothetical protein	-
KPU55_RS15995 (KPU55_15895)	5686..6120	+	435	WP_002365857	hypothetical protein	-
KPU55_RS16000 (KPU55_15900)	6167..6394	+	228	WP_002365858	hypothetical protein	-
KPU55_RS16005 (KPU55_15905)	6408..6704	+	297	WP_002365860	hypothetical protein	-
KPU55_RS16010 (KPU55_15910)	6719..7519	+	801	WP_002365861	PrgH	prgHb
KPU55_RS16015 (KPU55_15915)	7521..7874	+	354	WP_002365862	PrgI family mobile element protein	prgIc
KPU55_RS16020 (KPU55_15920)	7828..10218	+	2391	WP_002365863	VirB4-like conjugal transfer ATPase, CD1110 family	virb4
KPU55_RS16025 (KPU55_15925)	10230..12845	+	2616	WP_002365864	phage tail tip lysozyme	prgK
KPU55_RS16030 (KPU55_15930)	12869..13495	+	627	WP_242140985	hypothetical protein	prgL
KPU55_RS16035 (KPU55_15935)	13482..13727	+	246	WP_002382804	hypothetical protein	-
KPU55_RS16040 (KPU55_15940)	13711..14319	+	609	WP_242140987	PcfA protein	-
KPU55_RS16045 (KPU55_15945)	14480..14965	+	486	WP_016616031	PcfB family protein	gbs1365
KPU55_RS16050 (KPU55_15950)	14965..16794	+	1830	WP_242140990	VirD4-like conjugal transfer protein, CD1115 family	virb4
KPU55_RS16055 (KPU55_15955)	16845..19004	+	2160	WP_002365871	ArdC-like ssDNA-binding domain-containing protein	-
KPU55_RS16060 (KPU55_15960)	19037..19309	+	273	WP_002362409	LtrD	-
KPU55_RS16065 (KPU55_15965)	19555..19911	+	357	WP_002365872	plasmid mobilization relaxosome protein MobC	-
KPU55_RS16070 (KPU55_15970)	19912..21599	+	1688	Protein_19	relaxase/mobilization nuclease domain-containing protein	-

Host bacterium

• ID: 13333
• GenBank: NZ_CP076496
• Plasmid name: pUAMSEL3
• Incompatibility group: -
• Plasmid size: 53047 bp
• Coordinate of oriT [Strand]: 19439..19488 [+]
• Host baterium: Enterococcus faecalis strain UAMS_EL54

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -