Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 112895
• Name: oriT_pUAMSEL4
• Organism: Enterococcus faecalis strain UAMS_EL56
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP076492 (24436..24476 [-], 41 nt)
• oriT length: 41 nt
• IRs (inverted repeats): 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 41 nt

>oriT_pUAMSEL4
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_KPU60_RS16375_pUAMSEL4

• ID: 8298
• GenBank: WP_015382137
• Name: mobV_KPU60_RS16375_pUAMSEL4
• UniProt ID: L8BAH8
• Length: 409 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 409 a.a.
• Molecular weight: 48447.75 Da
• Isoelectric Point: 7.0106

>WP_015382137.1 MULTISPECIES: MobV family relaxase [Bacilli]
MSMIVARMQKMKAENLVGIGNHNQRKTKNHSNPDIDTSLSKLNYDLVDRTQNYKTDIENFINENKTTTRA
VRKDAVLVNEWIISSDKDFFDNLTESEIENFFERSKDYFAEKFGEKNIRYATVHLDESTPHMHMGIVPFD
KDNKLSAKRVFNRQALRDVQEELPKYLQDFQFEIERGQKGSERKNLTVPEFKKLKEEEREIKKELEIKKD
ELMAYTKENKIDKEIDITPIKEMEDVEIETDEKSLFGITKTKTVRQWTGNIVLSEKDYLKLRQKVNKGKQ
AEGKLEAILETDVYQENKELKNELKDQIDKNDKDIDDYNDLVKRYNNLYEENTSLKNQIGDLKEEIKLIY
QSTKRFFKDRISDFKAFKEVFKELADSISNISREKGLDSSFKKEFDRENKKKRTKGMSR

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

• AlphaFold DB: L8BAH8

Host bacterium

• ID: 13330
• GenBank: NZ_CP076492
• Plasmid name: pUAMSEL4
• Incompatibility group: -
• Plasmid size: 33659 bp
• Coordinate of oriT [Strand]: 24436..24476 [-]
• Host baterium: Enterococcus faecalis strain UAMS_EL56

Cargo genes

• Drug resistance gene: VanHAX, aadD
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -