Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   112894
Name   oriT_pUAMSEL3 in_silico
Organism   Enterococcus faecalis strain UAMS_EL56
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP076491 (18356..18405 [+], 50 nt)
oriT length   50 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_pUAMSEL3
AATATCGCAACATGCTAGCATGTTGCTCTGCTCGCAAAAAGAAAGCCTAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   8297 GenBank   WP_002365873
Name   Relaxase_KPU60_RS16055_pUAMSEL3 insolico UniProt ID   _
Length   561 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 561 a.a.        Molecular weight: 65469.22 Da        Isoelectric Point: 6.0174

>WP_002365873.1 relaxase/mobilization nuclease domain-containing protein [Enterococcus faecalis]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKRVFEQISDKIAAKEGAKIIEKSPKNS
HKKYTMWETESLYKEKIKSRLDFLLEQSSSINDFLEKAEALNLSVDFSKKWTTYRLLDEPQIKNTRSRSL
SKNDPTRYNYEKIVERLKENKSVLTLKEVVEKYVEKSEKTKNEFDYQLTIDEWQISHKTTRGYYVNVDFG
FGERGKVFIGAYKVDPLENGQYNIYVKRKDFFYLMNEKNADRNRYMTGETLIKQLRLYNGQTPLKKEPVM
RTIDELVNAINFLAANEIEDTRQLELLEEKLEAAFLEAEETLETLDEKMLELHQLSNLLLENELNGELEL
IQQKLKTLLPDATLAEFSYEDVRGEIEAIKTSQSLLENKLERTRNEINQLHEIQAVQKKETANQNNVKPK
L

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(37-296)


  Protein structure



No available structure.




Auxiliary protein

ID   4755 GenBank   WP_002365872
Name   WP_002365872_pUAMSEL3 insolico UniProt ID   _
Length   118 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 118 a.a.        Molecular weight: 13988.05 Da        Isoelectric Point: 9.6548

>WP_002365872.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Enterococcus]
MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVHSLNALVQSELNKLIKRKEQS

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(64-105)


  Protein structure



No available structure.




T4CP

ID   9620 GenBank   WP_242140990
Name   t4cp2_KPU60_RS16035_pUAMSEL3 insolico UniProt ID   _
Length   609 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 609 a.a.        Molecular weight: 70059.14 Da        Isoelectric Point: 6.4636

>WP_242140990.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFHRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNVAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEVKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRALEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(144-558)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2785..15711

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPU60_RS15965 (KPU60_15875) 2401..2757 + 357 WP_002365854 pheromone response system RNA-binding regulator PrgU -
KPU60_RS15970 (KPU60_15880) 2785..3642 + 858 WP_002401687 pheromone response LPXTG-anchored adhesin PrgC prgC
KPU60_RS15975 (KPU60_15885) 3684..4583 + 900 WP_025186515 hypothetical protein -
KPU60_RS15980 (KPU60_15890) 4603..5037 + 435 WP_002365857 hypothetical protein -
KPU60_RS15985 (KPU60_15895) 5084..5311 + 228 WP_002365858 hypothetical protein -
KPU60_RS15990 (KPU60_15900) 5325..5621 + 297 WP_002365860 hypothetical protein -
KPU60_RS15995 (KPU60_15905) 5636..6436 + 801 WP_002365861 PrgH prgHb
KPU60_RS16000 (KPU60_15910) 6438..6791 + 354 WP_002365862 PrgI family mobile element protein prgIc
KPU60_RS16005 (KPU60_15915) 6745..9135 + 2391 WP_002365863 VirB4-like conjugal transfer ATPase, CD1110 family virb4
KPU60_RS16010 (KPU60_15920) 9147..11762 + 2616 WP_002365864 phage tail tip lysozyme prgK
KPU60_RS16015 (KPU60_15925) 11786..12412 + 627 WP_242140985 hypothetical protein prgL
KPU60_RS16020 (KPU60_15930) 12399..12644 + 246 WP_002382804 hypothetical protein -
KPU60_RS16025 (KPU60_15935) 12628..13236 + 609 WP_242140987 PcfA protein -
KPU60_RS16030 (KPU60_15940) 13397..13882 + 486 WP_016616031 PcfB family protein gbs1365
KPU60_RS16035 (KPU60_15945) 13882..15711 + 1830 WP_242140990 VirD4-like conjugal transfer protein, CD1115 family virb4
KPU60_RS16040 (KPU60_15950) 15762..17921 + 2160 WP_002365871 ArdC-like ssDNA-binding domain-containing protein -
KPU60_RS16045 (KPU60_15955) 17954..18226 + 273 WP_002362409 LtrD -
KPU60_RS16050 (KPU60_15960) 18472..18828 + 357 WP_002365872 plasmid mobilization relaxosome protein MobC -
KPU60_RS16055 (KPU60_15965) 18829..20514 + 1686 WP_002365873 relaxase/mobilization nuclease domain-containing protein -

Host bacterium

ID   13329 GenBank   NZ_CP076491
Plasmid name   pUAMSEL3 Incompatibility group   -
Plasmid size   53193 bp Coordinate of oriT [Strand]   18356..18405 [+]
Host baterium   Enterococcus faecalis strain UAMS_EL56

Cargo genes

Drug resistance gene   -
Virulence gene   EF0149
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -