Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 112439 |
| Name | oriT_pCQ025-1  |
| Organism | Enterococcus faecalis strain CQ025 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP098419 (56892..56941 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | 34..35 |
| Conserved sequence flanking the nic site |
GCTTGCAAAA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pCQ025-1
AATATCGCAACATGCTAGCATGTTGCTCCGCTTGCAAAAAGAAAGCCTAC
AATATCGCAACATGCTAGCATGTTGCTCCGCTTGCAAAAAGAAAGCCTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 8053 | GenBank | WP_010784826 |
| Name | Relaxase_NAG09_RS14415_pCQ025-1 |
UniProt ID | A0A8E7CAC2 |
| Length | 561 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 561 a.a. Molecular weight: 65352.94 Da Isoelectric Point: 6.0135
>WP_010784826.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterococcus]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKIAAKEGAKIIEKSPKQT
HTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYG
YGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVM
QTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSNLLLENELQGDPEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQTLLESKLERTRNEINQLHEIQAVQEKEPEKQTDIKPK
L
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKIAAKEGAKIIEKSPKQT
HTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYG
YGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVM
QTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSNLLLENELQGDPEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQTLLESKLERTRNEINQLHEIQAVQEKEPEKQTDIKPK
L
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 4514 | GenBank | WP_002393737 |
| Name | WP_002393737_pCQ025-1 |
UniProt ID | A0A8E7CBI6 |
| Length | 118 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 118 a.a. Molecular weight: 13965.01 Da Isoelectric Point: 9.6545
>WP_002393737.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Enterococcus]
MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKDQS
MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKDQS
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 9281 | GenBank | WP_002401458 |
| Name | t4cp2_NAG09_RS14395_pCQ025-1 |
UniProt ID | _ |
| Length | 609 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 609 a.a. Molecular weight: 70173.19 Da Isoelectric Point: 6.1807
>WP_002401458.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 35505..54247
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| NAG09_RS14285 (NAG09_14280) | 31364..31744 | + | 381 | WP_002365885 | PrgR | - |
| NAG09_RS14290 (NAG09_14285) | 31795..32016 | + | 222 | WP_016616022 | hypothetical protein | - |
| NAG09_RS14295 (NAG09_14290) | 32318..32623 | + | 306 | WP_010715949 | hypothetical protein | - |
| NAG09_RS14300 (NAG09_14295) | 32634..35312 | + | 2679 | WP_251108128 | surface exclusion protein SEA1/PrgA | - |
| NAG09_RS14305 (NAG09_14300) | 35505..39422 | + | 3918 | WP_251108129 | LPXTG-anchored aggregation substance | prgB |
| NAG09_RS14310 (NAG09_14305) | 39484..39840 | + | 357 | WP_002365854 | pheromone response system RNA-binding regulator PrgU | - |
| NAG09_RS14315 (NAG09_14310) | 39868..40725 | + | 858 | WP_010709216 | pheromone response LPXTG-anchored adhesin PrgC | prgC |
| NAG09_RS14320 (NAG09_14315) | 40767..41666 | + | 900 | WP_002409287 | hypothetical protein | - |
| NAG09_RS14325 (NAG09_14320) | 41686..42120 | + | 435 | WP_002365857 | hypothetical protein | - |
| NAG09_RS14330 (NAG09_14325) | 42167..42394 | + | 228 | WP_002365858 | hypothetical protein | - |
| NAG09_RS14335 (NAG09_14330) | 42408..42704 | + | 297 | WP_002387740 | hypothetical protein | - |
| NAG09_RS14340 (NAG09_14335) | 42719..43519 | + | 801 | WP_002393746 | hypothetical protein | prgHb |
| NAG09_RS14345 (NAG09_14340) | 43521..43874 | + | 354 | WP_002367919 | PrgI family mobile element protein | prgIc |
| NAG09_RS14350 (NAG09_14345) | 43828..46218 | + | 2391 | WP_002365863 | VirB4-like conjugal transfer ATPase, CD1110 family | virb4 |
| NAG09_RS14355 (NAG09_14350) | 46230..48845 | + | 2616 | WP_002393744 | phage tail tip lysozyme | prgK |
| NAG09_RS14360 (NAG09_14355) | 48869..49495 | + | 627 | WP_002386490 | hypothetical protein | prgL |
| NAG09_RS14365 (NAG09_14360) | 49482..49727 | + | 246 | WP_002365866 | hypothetical protein | - |
| NAG09_RS14370 (NAG09_14365) | 49711..50319 | + | 609 | WP_016618746 | hypothetical protein | - |
| NAG09_RS14375 (NAG09_14370) | 50433..51188 | + | 756 | WP_077932946 | Fic family protein | - |
| NAG09_RS14380 (NAG09_14375) | 51205..51663 | + | 459 | WP_077932945 | single-stranded DNA-binding protein | - |
| NAG09_RS14385 (NAG09_14380) | 51709..51891 | + | 183 | WP_002367689 | hypothetical protein | - |
| NAG09_RS14390 (NAG09_14385) | 51933..52418 | + | 486 | WP_002386493 | PcfB family protein | gbs1365 |
| NAG09_RS14395 (NAG09_14390) | 52418..54247 | + | 1830 | WP_002401458 | VirD4-like conjugal transfer protein, CD1115 family | virb4 |
| NAG09_RS14400 (NAG09_14395) | 54298..56457 | + | 2160 | WP_251108130 | ArdC-like ssDNA-binding domain-containing protein | - |
| NAG09_RS14405 (NAG09_14400) | 56490..56762 | + | 273 | WP_002362409 | LtrD | - |
| NAG09_RS14410 (NAG09_14405) | 57008..57364 | + | 357 | WP_002393737 | plasmid mobilization relaxosome protein MobC | - |
| NAG09_RS14415 (NAG09_14410) | 57365..59050 | + | 1686 | WP_010784826 | relaxase/mobilization nuclease domain-containing protein | - |
Host bacterium
| ID | 12874 | GenBank | NZ_CP098419 |
| Plasmid name | pCQ025-1 | Incompatibility group | - |
| Plasmid size | 60399 bp | Coordinate of oriT [Strand] | 56892..56941 [+] |
| Host baterium | Enterococcus faecalis strain CQ025 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | prgB/asc10 |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA9 |