Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 111997 |
| Name | oriT_pE1123  |
| Organism | Enterococcus sp. E1123 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP075560 (20910..20959 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | 34..35 |
| Conserved sequence flanking the nic site |
GCTTGCAAAA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pE1123
AATATCGCAACATGCTAGCATGTTGCTCCGCTTGCAAAAAGAAAGCCTAC
AATATCGCAACATGCTAGCATGTTGCTCCGCTTGCAAAAAGAAAGCCTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 7734 | GenBank | WP_010784826 |
| Name | Relaxase_KI246_RS00115_pE1123 |
UniProt ID | A0A8E7CAC2 |
| Length | 561 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 561 a.a. Molecular weight: 65352.94 Da Isoelectric Point: 6.0135
>WP_010784826.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterococcus]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKIAAKEGAKIIEKSPKQT
HTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYG
YGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVM
QTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSNLLLENELQGDPEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQTLLESKLERTRNEINQLHEIQAVQEKEPEKQTDIKPK
L
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKIAAKEGAKIIEKSPKQT
HTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYG
YGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVM
QTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSNLLLENELQGDPEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQTLLESKLERTRNEINQLHEIQAVQEKEPEKQTDIKPK
L
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 4202 | GenBank | WP_002393737 |
| Name | WP_002393737_pE1123 |
UniProt ID | A0A8E7CBI6 |
| Length | 118 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 118 a.a. Molecular weight: 13965.01 Da Isoelectric Point: 9.6545
>WP_002393737.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Enterococcus]
MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKDQS
MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKDQS
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 8842 | GenBank | WP_126263104 |
| Name | t4cp2_KI246_RS00095_pE1123 |
UniProt ID | _ |
| Length | 609 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 609 a.a. Molecular weight: 70188.16 Da Isoelectric Point: 6.1807
>WP_126263104.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Enterococcus]
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSEQKEETHV
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSEQKEETHV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 3887..18266
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KI246_RS00010 (KI246_00010) | 3503..3859 | + | 357 | WP_002367672 | pheromone response system RNA-binding regulator PrgU | - |
| KI246_RS00015 (KI246_00015) | 3887..4744 | + | 858 | WP_002367673 | pheromone response LPXTG-anchored adhesin PrgC | prgC |
| KI246_RS00020 (KI246_00020) | 4786..5685 | + | 900 | WP_002409287 | hypothetical protein | - |
| KI246_RS00025 (KI246_00025) | 5705..6139 | + | 435 | WP_002365857 | hypothetical protein | - |
| KI246_RS00030 (KI246_00030) | 6186..6413 | + | 228 | WP_002365858 | hypothetical protein | - |
| KI246_RS00035 (KI246_00035) | 6427..6723 | + | 297 | WP_002387740 | hypothetical protein | - |
| KI246_RS00040 (KI246_00040) | 6738..7538 | + | 801 | WP_002393746 | hypothetical protein | prgHb |
| KI246_RS00045 (KI246_00045) | 7540..7893 | + | 354 | WP_002367919 | PrgI family mobile element protein | prgIc |
| KI246_RS00050 (KI246_00050) | 7847..10237 | + | 2391 | WP_002365863 | VirB4-like conjugal transfer ATPase, CD1110 family | virb4 |
| KI246_RS00055 (KI246_00055) | 10249..12864 | + | 2616 | WP_002410288 | phage tail tip lysozyme | prgK |
| KI246_RS00060 (KI246_00060) | 12888..13514 | + | 627 | WP_002386490 | hypothetical protein | prgL |
| KI246_RS00065 (KI246_00065) | 13501..13746 | + | 246 | WP_002365866 | hypothetical protein | - |
| KI246_RS00070 (KI246_00070) | 13730..14338 | + | 609 | WP_016618746 | hypothetical protein | - |
| KI246_RS00075 (KI246_00075) | 14452..15207 | + | 756 | WP_077932946 | Fic family protein | - |
| KI246_RS00080 (KI246_00080) | 15224..15682 | + | 459 | WP_077932945 | single-stranded DNA-binding protein | - |
| KI246_RS00085 (KI246_00085) | 15728..15910 | + | 183 | WP_002367689 | hypothetical protein | - |
| KI246_RS00090 (KI246_00090) | 15952..16437 | + | 486 | WP_002386493 | PcfB family protein | gbs1365 |
| KI246_RS00095 (KI246_00095) | 16437..18266 | + | 1830 | WP_126263104 | VirD4-like conjugal transfer protein, CD1115 family | virb4 |
| KI246_RS00100 (KI246_00100) | 18316..20475 | + | 2160 | WP_126263105 | ArdC-like ssDNA-binding domain-containing protein | - |
| KI246_RS00105 (KI246_00105) | 20508..20780 | + | 273 | WP_002362409 | LtrD | - |
| KI246_RS00110 (KI246_00110) | 21026..21382 | + | 357 | WP_002393737 | plasmid mobilization relaxosome protein MobC | - |
| KI246_RS00115 (KI246_00115) | 21383..23068 | + | 1686 | WP_010784826 | relaxase/mobilization nuclease domain-containing protein | - |
Host bacterium
| ID | 12432 | GenBank | NZ_CP075560 |
| Plasmid name | pE1123 | Incompatibility group | - |
| Plasmid size | 79681 bp | Coordinate of oriT [Strand] | 20910..20959 [+] |
| Host baterium | Enterococcus sp. E1123 |
Cargo genes
| Drug resistance gene | ant(9)-Ia, erm(A), fexA, optrA |
| Virulence gene | prgB/asc10 |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA9 |