Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   111993
Name   oriT_pEX_Surv232-KPC2 in_silico
Organism   Enterobacter hormaechei subsp. xiangfangensis strain Survcare232
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JAFHZD010000135 (19363..19462 [-], 100 nt)
oriT length   100 nt
IRs (inverted repeats)      77..82, 90..95  (AAAAAA..TTTTTT)
 78..83, 90..95  (AAAAAA..TTTTTT)
 79..84, 90..95  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 100 nt

>oriT_pEX_Surv232-KPC2
TATTTATTTTTTTATCTTTTAAATCAGTACGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGAAAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   7732 GenBank   WP_012561142
Name   mobF_KKV86_RS24590_pEX_Surv232-KPC2 insolico UniProt ID   A0A1S6KKJ9
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120195.37 Da        Isoelectric Point: 6.5230

>WP_012561142.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKADMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

AAA domain (AAA_30)

(477-653)

TrwC relaxase (TrwC)

(16-287)


  Protein structure


Source ID Structure
AlphaFold DB A0A1S6KKJ9


Auxiliary protein

ID   4199 GenBank   WP_001749975
Name   WP_001749975_pEX_Surv232-KPC2 insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   8839 GenBank   WP_000342688
Name   t4cp2_KKV86_RS24585_pEX_Surv232-KPC2 insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 53690..64019

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKV86_RS24755 49665..50525 + 861 WP_000027057 broad-spectrum class A beta-lactamase TEM-1 -
KKV86_RS24760 50784..51665 + 882 WP_004199234 carbapenem-hydrolyzing class A beta-lactamase KPC-2 -
KKV86_RS24765 51915..53234 - 1320 WP_004152397 IS1182-like element ISKpn6 family transposase -
KKV86_RS24770 53586..53690 - 105 Protein_59 phospholipase D family protein -
KKV86_RS24775 53690..54685 - 996 WP_012561144 ATPase, T2SS/T4P/T4SS family virB11
KKV86_RS24780 54727..55887 - 1161 WP_000101710 type IV secretion system protein VirB10 virB10
KKV86_RS24785 55887..56771 - 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
KKV86_RS24790 56782..57480 - 699 WP_000646594 type IV secretion system protein virB8
KKV86_RS24795 57699..58739 - 1041 WP_001749958 type IV secretion system protein virB6
KKV86_RS24800 58755..58982 - 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
KKV86_RS24805 58990..59703 - 714 WP_001749960 type IV secretion system protein virB5
KKV86_RS24810 59721..62189 - 2469 WP_223669807 VirB4 family type IV secretion/conjugal transfer ATPase virb4
KKV86_RS24815 62321..62638 - 318 WP_000496058 VirB3 family type IV secretion system protein virB3
KKV86_RS24820 62689..62982 - 294 WP_001749962 hypothetical protein virB2
KKV86_RS24825 62992..63273 - 282 WP_016338364 transcriptional repressor KorA -
KKV86_RS24830 63282..64019 - 738 WP_013279384 lytic transglycosylase domain-containing protein virB1
KKV86_RS24835 64128..64433 + 306 WP_012561154 H-NS family nucleoid-associated regulatory protein -
KKV86_RS24840 64449..64793 + 345 WP_012561155 hypothetical protein -
KKV86_RS24845 64790..65104 + 315 WP_016338363 TrbM/KikA/MpfK family conjugal transfer protein -
KKV86_RS24850 65140..65451 + 312 WP_013279382 hypothetical protein -
KKV86_RS24855 65507..65767 + 261 WP_016359294 hypothetical protein -
KKV86_RS24860 65809..66777 - 969 WP_074182791 IS5 family transposase -
KKV86_RS24865 66795..66926 - 132 WP_255255389 hypothetical protein -
KKV86_RS24870 66861..67214 + 354 WP_225622145 restriction endonuclease -
KKV86_RS24875 67219..67425 + 207 WP_001749967 hypothetical protein -
KKV86_RS24880 67436..67720 - 285 Protein_81 IS1 family transposase -

Host bacterium

ID   12428 GenBank   NZ_JAFHZD010000135
Plasmid name   pEX_Surv232-KPC2 Incompatibility group   IncN
Plasmid size   70648 bp Coordinate of oriT [Strand]   19363..19462 [-]
Host baterium   Enterobacter hormaechei subsp. xiangfangensis strain Survcare232

Cargo genes

Drug resistance gene   aph(6)-Id, aph(3'')-Ib, dfrA19, sul1, qacE, ARR-3, catB3, blaOXA-1, aac(6')-Ib-cr, aac(3)-IId, blaTEM-1B, blaKPC-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -