Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   111992
Name   oriT_pEX_Surv257-KPC2 in_silico
Organism   Enterobacter hormaechei subsp. xiangfangensis strain Survcare257
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JAFIAB010000074 (19363..19462 [-], 100 nt)
oriT length   100 nt
IRs (inverted repeats)      77..82, 90..95  (AAAAAA..TTTTTT)
 78..83, 90..95  (AAAAAA..TTTTTT)
 79..84, 90..95  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 100 nt

>oriT_pEX_Surv257-KPC2
TATTTATTTTTTTATCTTTTAAATCAGTACGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGAAAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   7731 GenBank   WP_012561142
Name   mobF_KKS79_RS24200_pEX_Surv257-KPC2 insolico UniProt ID   A0A1S6KKJ9
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120195.37 Da        Isoelectric Point: 6.5230

>WP_012561142.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKADMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

AAA domain (AAA_30)

(477-653)

TrwC relaxase (TrwC)

(16-287)


  Protein structure


Source ID Structure
AlphaFold DB A0A1S6KKJ9


Auxiliary protein

ID   4198 GenBank   WP_001749975
Name   WP_001749975_pEX_Surv257-KPC2 insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   8838 GenBank   WP_000342688
Name   t4cp2_KKS79_RS24195_pEX_Surv257-KPC2 insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 54910..65239

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKS79_RS24375 50885..51745 + 861 WP_000027057 broad-spectrum class A beta-lactamase TEM-1 -
KKS79_RS24380 52004..52885 + 882 WP_004199234 carbapenem-hydrolyzing class A beta-lactamase KPC-2 -
KKS79_RS24385 53135..54454 - 1320 WP_004152397 IS1182-like element ISKpn6 family transposase -
KKS79_RS24390 54806..54910 - 105 Protein_61 phospholipase D family protein -
KKS79_RS24395 54910..55905 - 996 WP_012561144 ATPase, T2SS/T4P/T4SS family virB11
KKS79_RS24400 55947..57107 - 1161 WP_000101710 type IV secretion system protein VirB10 virB10
KKS79_RS24405 57107..57991 - 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
KKS79_RS24410 58002..58700 - 699 WP_000646594 type IV secretion system protein virB8
KKS79_RS24415 58919..59959 - 1041 WP_001749958 type IV secretion system protein virB6
KKS79_RS24420 59975..60202 - 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
KKS79_RS24425 60210..60923 - 714 WP_001749960 type IV secretion system protein virB5
KKS79_RS24430 60941..63409 - 2469 WP_223669807 VirB4 family type IV secretion/conjugal transfer ATPase virb4
KKS79_RS24435 63541..63858 - 318 WP_000496058 VirB3 family type IV secretion system protein virB3
KKS79_RS24440 63909..64202 - 294 WP_001749962 hypothetical protein virB2
KKS79_RS24445 64212..64493 - 282 WP_016338364 transcriptional repressor KorA -
KKS79_RS24450 64502..65239 - 738 WP_013279384 lytic transglycosylase domain-containing protein virB1
KKS79_RS24455 65348..65653 + 306 WP_012561154 H-NS family nucleoid-associated regulatory protein -
KKS79_RS24460 65669..66013 + 345 WP_012561155 hypothetical protein -
KKS79_RS24465 66010..66324 + 315 WP_016338363 TrbM/KikA/MpfK family conjugal transfer protein -
KKS79_RS24470 66438..66671 + 234 WP_012561157 hypothetical protein -
KKS79_RS24475 66727..66987 + 261 WP_016359294 hypothetical protein -
KKS79_RS24480 67029..67997 - 969 WP_016151349 IS5 family transposase -
KKS79_RS24485 68081..68434 + 354 WP_225622145 restriction endonuclease -
KKS79_RS24490 68439..68645 + 207 WP_001749967 hypothetical protein -
KKS79_RS24495 68656..68928 - 273 Protein_82 IS1 family transposase -

Host bacterium

ID   12427 GenBank   NZ_JAFIAB010000074
Plasmid name   pEX_Surv257-KPC2 Incompatibility group   IncN
Plasmid size   71867 bp Coordinate of oriT [Strand]   19363..19462 [-]
Host baterium   Enterobacter hormaechei subsp. xiangfangensis strain Survcare257

Cargo genes

Drug resistance gene   aph(6)-Id, aph(3'')-Ib, dfrA19, sul1, qacE, ARR-3, catB3, blaOXA-1, aac(6')-Ib-cr, aac(3)-IId, blaTEM-1B, blaKPC-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -