Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   111991
Name   oriT_pEX_Surv203-KPC2 in_silico
Organism   Enterobacter hormaechei subsp. xiangfangensis strain Survcare203
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JAFHYL010000047 (19363..19462 [-], 100 nt)
oriT length   100 nt
IRs (inverted repeats)      77..82, 90..95  (AAAAAA..TTTTTT)
 78..83, 90..95  (AAAAAA..TTTTTT)
 79..84, 90..95  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 100 nt

>oriT_pEX_Surv203-KPC2
TATTTATTTTTTTATCTTTTAAATCAGTACGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGAAAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   7730 GenBank   WP_012561142
Name   mobF_KKV58_RS23880_pEX_Surv203-KPC2 insolico UniProt ID   A0A1S6KKJ9
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120195.37 Da        Isoelectric Point: 6.5230

>WP_012561142.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKADMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

AAA domain (AAA_30)

(477-653)

TrwC relaxase (TrwC)

(16-287)


  Protein structure


Source ID Structure
AlphaFold DB A0A1S6KKJ9


Auxiliary protein

ID   4197 GenBank   WP_001749975
Name   WP_001749975_pEX_Surv203-KPC2 insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   8837 GenBank   WP_000342688
Name   t4cp2_KKV58_RS23875_pEX_Surv203-KPC2 insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 42812..53141

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKV58_RS23975 38787..39647 + 861 WP_000027057 broad-spectrum class A beta-lactamase TEM-1 -
KKV58_RS23980 39906..40787 + 882 WP_004199234 carbapenem-hydrolyzing class A beta-lactamase KPC-2 -
KKV58_RS23985 41037..42356 - 1320 WP_004152397 IS1182-like element ISKpn6 family transposase -
KKV58_RS23990 42708..42812 - 105 Protein_45 phospholipase D family protein -
KKV58_RS23995 42812..43807 - 996 WP_012561144 ATPase, T2SS/T4P/T4SS family virB11
KKV58_RS24000 43849..45009 - 1161 WP_000101710 type IV secretion system protein VirB10 virB10
KKV58_RS24005 45009..45893 - 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
KKV58_RS24010 45904..46602 - 699 WP_000646594 type IV secretion system protein virB8
KKV58_RS24015 46821..47861 - 1041 WP_001749958 type IV secretion system protein virB6
KKV58_RS24020 47877..48104 - 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
KKV58_RS24025 48112..48825 - 714 WP_001749960 type IV secretion system protein virB5
KKV58_RS24030 48843..51311 - 2469 WP_223669807 VirB4 family type IV secretion/conjugal transfer ATPase virb4
KKV58_RS24035 51443..51760 - 318 WP_000496058 VirB3 family type IV secretion system protein virB3
KKV58_RS24040 51811..52104 - 294 WP_001749962 hypothetical protein virB2
KKV58_RS24045 52114..52395 - 282 WP_016338364 transcriptional repressor KorA -
KKV58_RS24050 52404..53141 - 738 WP_013279384 lytic transglycosylase domain-containing protein virB1
KKV58_RS24055 53250..53555 + 306 WP_012561154 H-NS family nucleoid-associated regulatory protein -
KKV58_RS24060 53571..53915 + 345 WP_012561155 hypothetical protein -
KKV58_RS24065 53912..54226 + 315 WP_016338363 TrbM/KikA/MpfK family conjugal transfer protein -
KKV58_RS24070 54262..54573 + 312 WP_013279382 hypothetical protein -
KKV58_RS24075 54629..54889 + 261 WP_016359294 hypothetical protein -
KKV58_RS24080 54931..55899 - 969 WP_016151349 IS5 family transposase -
KKV58_RS24085 55983..56336 + 354 WP_225622145 restriction endonuclease -
KKV58_RS24090 56341..56547 + 207 WP_001749967 hypothetical protein -
KKV58_RS24095 56558..56830 - 273 Protein_66 IS1 family transposase -

Host bacterium

ID   12426 GenBank   NZ_JAFHYL010000047
Plasmid name   pEX_Surv203-KPC2 Incompatibility group   IncN
Plasmid size   59769 bp Coordinate of oriT [Strand]   19363..19462 [-]
Host baterium   Enterobacter hormaechei subsp. xiangfangensis strain Survcare203

Cargo genes

Drug resistance gene   aph(6)-Id, aph(3'')-Ib, aac(3)-IId, blaTEM-1B, blaKPC-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -