Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 111888 |
| Name | oriT_pKPC_CAV1176  |
| Organism | Enterobacter hormaechei strain CAV1176 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP011661 (11998..12091 [+], 94 nt) |
| oriT length | 94 nt |
| IRs (inverted repeats) | 73..78, 84..89 (AAAAAA..TTTTTT) 27..34, 37..44 (AGCGTGAT..ATCACGCT) 13..19, 31..37 (TAAATCA..TGATTTA) |
| Location of nic site | 55..56 |
| Conserved sequence flanking the nic site |
GGTGTATAGC |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 94 nt
>oriT_pKPC_CAV1176
TTTTTTTTCTTTTAAATCAGTGGTATAGCGTGATTTATCACGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG
TTTTTTTTCTTTTAAATCAGTGGTATAGCGTGATTTATCACGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 8796 | GenBank | WP_029074264 |
| Name | t4cp2_AB284_RS00745_pKPC_CAV1176 |
UniProt ID | _ |
| Length | 662 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 662 a.a. Molecular weight: 73142.66 Da Isoelectric Point: 6.5145
>WP_029074264.1 MULTISPECIES: conjugal transfer protein TraG [Pseudomonadota]
MRGGRILWGQIAVVFTIVLVMTWAATQWVAFRLGFQPQLGNPWFELVGLPVYYPPAFFWWWFSFDAYAPA
IFVEGGIIAVSGGFLAIAAAILMSIIRAREARNVATYGSARWAEDREIRTAGLLGPDGVLLGRYNRDYLR
HDGPEHVLCFAPTRSGKGVGLVVPTLLTWSASAIVHDIKGENWTLTAGFRAKHGRVLLFDPTNARSSAYN
PLLEVRQGEWEVRDVQNIADILVDPEGSLDKRNHWEKTSHSLLVGAILHVLYAEKDKTLAGVANFLSDPR
RPVEATLRAMMDTPHLGEAGVHPVIASSARELLNKSENERSGVLSTAMSFLGLYRDPVVAQVTARCDWRI
ADLVGSRRPVTLYLVVPPSDINRTKPLIRLILNQIGRRLTEELTTSGKRHRLLLMLDEFPALGRLDFFES
ALAFMAGYGIKGFLIAQSLNQIERAYGPNNAILDNCHVRVSFATNDERTAKRVSDALGTATELRDSTNYA
GHRLAPWLGHLMVSRQETARPLLTPGEIMQLPPTDEIVMVAGTPPIRATKARYFEDARFQERILTPPDLV
AAPLAPSPSADDWSGRVVAAGSRSATVAADATEGDPANAGIRREPELPEHEEIVTPPPSPEQEFDILDDE
PDVDAAKARTIRQRMRMVARQVAMNPDDGIEL
MRGGRILWGQIAVVFTIVLVMTWAATQWVAFRLGFQPQLGNPWFELVGLPVYYPPAFFWWWFSFDAYAPA
IFVEGGIIAVSGGFLAIAAAILMSIIRAREARNVATYGSARWAEDREIRTAGLLGPDGVLLGRYNRDYLR
HDGPEHVLCFAPTRSGKGVGLVVPTLLTWSASAIVHDIKGENWTLTAGFRAKHGRVLLFDPTNARSSAYN
PLLEVRQGEWEVRDVQNIADILVDPEGSLDKRNHWEKTSHSLLVGAILHVLYAEKDKTLAGVANFLSDPR
RPVEATLRAMMDTPHLGEAGVHPVIASSARELLNKSENERSGVLSTAMSFLGLYRDPVVAQVTARCDWRI
ADLVGSRRPVTLYLVVPPSDINRTKPLIRLILNQIGRRLTEELTTSGKRHRLLLMLDEFPALGRLDFFES
ALAFMAGYGIKGFLIAQSLNQIERAYGPNNAILDNCHVRVSFATNDERTAKRVSDALGTATELRDSTNYA
GHRLAPWLGHLMVSRQETARPLLTPGEIMQLPPTDEIVMVAGTPPIRATKARYFEDARFQERILTPPDLV
AAPLAPSPSADDWSGRVVAAGSRSATVAADATEGDPANAGIRREPELPEHEEIVTPPPSPEQEFDILDDE
PDVDAAKARTIRQRMRMVARQVAMNPDDGIEL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 8797 | GenBank | WP_029074259 |
| Name | trbE_AB284_RS00780_pKPC_CAV1176 |
UniProt ID | _ |
| Length | 830 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 830 a.a. Molecular weight: 90719.49 Da Isoelectric Point: 5.3930
>WP_029074259.1 MULTISPECIES: conjugal transfer protein TrbE [Pseudomonadota]
MMNLSEYRRTAARLADYLPWAALLGSGVVLNKDGSFQRTAKFRGPDLDSAVAAELVAVAGRINNAVRRLG
SGWSIFVEAQRSEAATYPDSTFPDAASALVDAERKAGFEEAGTHFVSGYFLTFLWLPPAEEAARAETWLY
EGREKTGVDPWELLRGFIDRTDRVLALLDGFMPECRWMDDGATLTYLHSTISTNRHRVRVPEVPMHLDAL
LADQPLTGGLEPRLGTKHLRVLTIVGFPTATTPGLLDEMNRLPFPYRWSTRAILLDKTDATRLLTRIRRQ
WFAKRKSIAAILKEVMTNEASALVDTDAANKALDADMALQELGADVAGMAYVTATVTVWDADPRLADEKL
RLVEKIVQGRDFTAMPESVNAVDAWLGSLPGHAYANVRQPPISTLNLAHMIPLSAVWAGPERDEHFGAPP
LLFGKTEGSTPFRLSLHVGDVGHTLVVGPTGAGKSVLLALMALQFRRYAGSQVFAFDFGGSIRAAALAMG
GDWHDLGGGLTEGAEASVSLQPLARIHDTYERAWAADWIAAILMREGIAITPEVKEYLWTALTSLASAPV
EERTITGLAVLLQSNDLKQALRPFCVGGAYGRLLDAEAEHLGSADVQAFEIEGLVGTGAAPAVLSYLFHR
IGDRLDGRPTLLIIDEGWLALDDEGFAGQLREWLKTLRKKNASVIFATQSLSDIDGSNIAPAIIESCPTR
LLLPNERAIEPQITEIYRRFGLNDRQIEILARATPKRDYYCQSRRGNRLFELGLSEIGLALCAASAKSDQ
TLIAQIVAEHGRDGFLAAWLRARGVEWAAELIPNLTNLADRPESAAPARLDTHPTQEILP
MMNLSEYRRTAARLADYLPWAALLGSGVVLNKDGSFQRTAKFRGPDLDSAVAAELVAVAGRINNAVRRLG
SGWSIFVEAQRSEAATYPDSTFPDAASALVDAERKAGFEEAGTHFVSGYFLTFLWLPPAEEAARAETWLY
EGREKTGVDPWELLRGFIDRTDRVLALLDGFMPECRWMDDGATLTYLHSTISTNRHRVRVPEVPMHLDAL
LADQPLTGGLEPRLGTKHLRVLTIVGFPTATTPGLLDEMNRLPFPYRWSTRAILLDKTDATRLLTRIRRQ
WFAKRKSIAAILKEVMTNEASALVDTDAANKALDADMALQELGADVAGMAYVTATVTVWDADPRLADEKL
RLVEKIVQGRDFTAMPESVNAVDAWLGSLPGHAYANVRQPPISTLNLAHMIPLSAVWAGPERDEHFGAPP
LLFGKTEGSTPFRLSLHVGDVGHTLVVGPTGAGKSVLLALMALQFRRYAGSQVFAFDFGGSIRAAALAMG
GDWHDLGGGLTEGAEASVSLQPLARIHDTYERAWAADWIAAILMREGIAITPEVKEYLWTALTSLASAPV
EERTITGLAVLLQSNDLKQALRPFCVGGAYGRLLDAEAEHLGSADVQAFEIEGLVGTGAAPAVLSYLFHR
IGDRLDGRPTLLIIDEGWLALDDEGFAGQLREWLKTLRKKNASVIFATQSLSDIDGSNIAPAIIESCPTR
LLLPNERAIEPQITEIYRRFGLNDRQIEILARATPKRDYYCQSRRGNRLFELGLSEIGLALCAASAKSDQ
TLIAQIVAEHGRDGFLAAWLRARGVEWAAELIPNLTNLADRPESAAPARLDTHPTQEILP
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 53032..62768
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AB284_RS00740 (AB284_00700) | 48606..49112 | - | 507 | WP_031623797 | helix-turn-helix domain-containing protein | - |
| AB284_RS00745 (AB284_00705) | 49382..51370 | + | 1989 | WP_029074264 | conjugal transfer protein TraG | - |
| AB284_RS00750 (AB284_00710) | 51381..51854 | + | 474 | WP_029074263 | hypothetical protein | - |
| AB284_RS00755 (AB284_00715) | 51931..52695 | - | 765 | WP_158581548 | alpha/beta fold hydrolase | - |
| AB284_RS00760 | 52763..52885 | + | 123 | Protein_52 | CopG family transcriptional regulator | - |
| AB284_RS00765 (AB284_00720) | 53032..54027 | + | 996 | WP_029074262 | P-type conjugative transfer ATPase TrbB | virB11 |
| AB284_RS00770 (AB284_00725) | 54024..54356 | + | 333 | WP_029074261 | TrbC/VirB2 family protein | virB2 |
| AB284_RS00775 (AB284_00730) | 54356..54637 | + | 282 | WP_029074260 | VirB3 family type IV secretion system protein | virB3 |
| AB284_RS00780 (AB284_00735) | 54648..57140 | + | 2493 | WP_029074259 | conjugal transfer protein TrbE | virb4 |
| AB284_RS00785 (AB284_00740) | 57137..57913 | + | 777 | WP_029074258 | P-type conjugative transfer protein TrbJ | virB5 |
| AB284_RS00790 (AB284_00745) | 57928..58194 | + | 267 | WP_031623918 | putative entry exclusion protein TrbK-alt | - |
| AB284_RS00795 (AB284_00750) | 58197..59552 | + | 1356 | WP_029074256 | P-type conjugative transfer protein TrbL | virB6 |
| AB284_RS00800 (AB284_00755) | 59549..60238 | + | 690 | WP_031623919 | conjugal transfer protein TrbF | virB8 |
| AB284_RS00805 (AB284_00760) | 60244..60621 | - | 378 | WP_029074878 | nuclear transport factor 2 family protein | - |
| AB284_RS00810 (AB284_00765) | 60718..61635 | + | 918 | WP_200523655 | P-type conjugative transfer protein TrbG | virB9 |
| AB284_RS00815 (AB284_00770) | 61632..62768 | + | 1137 | WP_031623920 | TrbI/VirB10 family protein | virB10 |
| AB284_RS00820 (AB284_00775) | 62771..62992 | + | 222 | WP_029074252 | DUF2274 domain-containing protein | - |
| AB284_RS00825 (AB284_00780) | 63128..63445 | + | 318 | WP_029074251 | hypothetical protein | - |
| AB284_RS00830 (AB284_00785) | 63442..63795 | + | 354 | WP_029074250 | DUF6516 family protein | - |
| AB284_RS00835 (AB284_00790) | 63792..64190 | + | 399 | WP_029074249 | helix-turn-helix domain-containing protein | - |
| AB284_RS00840 (AB284_00795) | 64187..65833 | + | 1647 | WP_031623921 | recombinase family protein | - |
| AB284_RS00845 (AB284_00800) | 65814..66137 | + | 324 | Protein_69 | IS21 family transposase | - |
| AB284_RS00850 (AB284_00805) | 66124..66909 | + | 786 | WP_000983249 | IS21-like element IS1326 family helper ATPase IstB | - |
Host bacterium
| ID | 12323 | GenBank | NZ_CP011661 |
| Plasmid name | pKPC_CAV1176 | Incompatibility group | IncR |
| Plasmid size | 90452 bp | Coordinate of oriT [Strand] | 11998..12091 [+] |
| Host baterium | Enterobacter hormaechei strain CAV1176 |
Cargo genes
| Drug resistance gene | blaKPC-2, blaTEM-1B, floR, tet(G), sul1, qacE, ant(3'')-Ia |
| Virulence gene | - |
| Metal resistance gene | merE, merD, merB, merR2 |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |