Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   111860
Name   oriT_pJNFE1126A in_silico
Organism   Bacillus subtilis strain JNFE1126
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP070486 (8452..8813 [-], 362 nt)
oriT length   362 nt
IRs (inverted repeats)      188..193, 198..203  (CACCAT..ATGGTG)
 171..178, 180..187  (AAACCGCA..TGCGGTTT)
 116..122, 130..136  (ACATAAA..TTTATGT)
 84..89, 101..106  (ATTTAG..CTAAAT)
 84..89, 92..97  (ATTTAG..CTAAAT)
 62..67, 75..80  (CATAAA..TTTATG)
 48..54, 65..71  (TTATTTT..AAAATAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 362 nt

>oriT_pJNFE1126A
AAAGAGCAATCTCGTCATCGAAGACTAAATTTCTGTATGGAAAACAGTTATTTTTCGTGTGCATAAAATAAAGATTTATGTGCATTTAGTTCTAAATCACCTAAATAATGGTTGAACATAAATGTTTTTTTTATGTGCATTCAGCAATAAATCTGGTACCACGAAAAAACAAACCGCACTGCGGTTTCACCATGCAAATGGTGCCAGTTTCCCCTTATGCTCTTTCCTTTTCCACCCCCTCCTTCCTTCGGAATCGGGGGCCGGCTTTTTGCTGCCGCAAAAAGCCTTGGAAAAAGAAACACTTATTTGAACAGATCGTTGCAAAGTAATGTGCAGAAATCGATGAAAAGGAGCGTTAACAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   7685 GenBank   WP_013603221
Name   mobP2_JWY35_RS22050_pJNFE1126A insolico UniProt ID   E9RJ23
Length   410 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 48596.08 Da        Isoelectric Point: 10.0206

>WP_013603221.1 MULTISPECIES: MobP2 family relaxase [Bacillaceae]
MDSPGVVLVSKYVSGKSTKFSKYVNYINRDEAVRTEKFQTYNVNKLDGYNQYMGNPEKSSGIFTQHKDSL
SPVEKNQLKEIFRQAQKNDSVMWQDVISFDNKWLEERGIYNSQTGWVNEGAIQNSIRKGMEVLLREEQLE
QSGVWSAAIHYNTDNIHVHIALVEPNPTKEYGVFTNKKTGEVYQARRGNRKLKTLDKMKSKVANTLMDRD
KELSKISQLIHDRIAPKGLKFQPRLDTYMTKMYNHIYENLPEDMRLWKYNNNALNNIRPEIDSVITMYIQ
KYHPEDYKELDQSLKEEMEFRKSVYGDGPKQVERYKEYRKNKHKELYTKLGNSMLKEMAEIRRRESQSKQ
MNRSGTYAPSSVSNRQWDTKGRRIKRSDINKIKHALDKDYQSMKNMRKYQQMQYEMEQSR

  Protein domains


Predicted by InterproScan.

MobL relaxases (MobL)

(3-389)


  Protein structure


Source ID Structure
AlphaFold DB E9RJ23


T4CP

ID   8782 GenBank   WP_013603210
Name   t4cp2_JWY35_RS22105_pJNFE1126A insolico UniProt ID   _
Length   786 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 786 a.a.        Molecular weight: 90624.17 Da        Isoelectric Point: 4.9504

>WP_013603210.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Bacillus]
MHKEFKQWKKVFADKYFLIAFTLFCYVAVTCITNFFIHLLKQIPVILSSFKNFDQSQLSSPFSSMTWKWF
FEFDWSMGIVYGVVYVLASIFILRQVYRFRIAFRDINKQTKGTARWTEIMEIQETYKAVKDDDNEYEGSA
GMPIVHFNDHLYVDTNSTHTLVVASTQSGKTETYSYPYLDVIMRAKEKDSVVITDIKGDMLKNTRAEFEK
YGYEVMCFNLLNPYWGMAYNPLELVKQAYLKKDYSKAQMLCNTLSYSLFHNDKSHADPMWENASIALVNA
LILAVCDLCIKNGQPENITMYTLTVMLNELGSNPDEDGYTRLDHFFGNLPPSHPAKLQYGTIQFSQGITR
SGIYTGTMAKLKNYTYDTIARMTARNDLNIEDLAYGEKPVALFIVYPDWDDSNYTIISTFLSQVNAVLSE
KATLSKESTLPRKVRFLFEEVANIPPIEGLNRSLAVGLSRGMLYTLVIQNVSQLRDVYGDDMAVAIMGNL
GNQIYIMSDEWEDAEKFSEKLGVTTVISADRQGDLMDVHKSYSEREEERPLMLPDELRRLKKGEWVVLRT
KKREDLKRNRVVPYPIFASLDNGTNMLHRYEYLMHRFDNKIALGDLGFRGEHETLDLESLLIEFEFNEPV
KEERKSKPGKGKKQKAKKAPGEPVSSEFPLPEEPIGFTESSEGMDSLFASIPVENMNEHGFYEDPPMNES
LHEDDLEASEEDIIMNSLEEVYETSTPISDAIKPDHYVYIKLLAHKQLSESEYAYFDSLKTVEELRAFFR
APEKKEIYEQIKTHIE

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(148-567)

  Protein structure



No available structure.




Host bacterium

ID   12295 GenBank   NZ_CP070486
Plasmid name   pJNFE1126A Incompatibility group   -
Plasmid size   64309 bp Coordinate of oriT [Strand]   8452..8813 [-]
Host baterium   Bacillus subtilis strain JNFE1126

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -