Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 111474 |
| Name | oriT_pTMSNI47-1  |
| Organism | Klebsiella quasipneumoniae strain SNI47 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_AP019688 (43137..43287 [+], 151 nt) |
| oriT length | 151 nt |
| IRs (inverted repeats) | 93..98, 106..111 (TGGCCT..AGGCCA) 12..17, 24..29 (AACCCT..AGGGTT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 151 nt
>oriT_pTMSNI47-1
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 7460 | GenBank | WP_151819900 |
| Name | mobH_TMSI_RS27550_pTMSNI47-1 |
UniProt ID | _ |
| Length | 992 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 992 a.a. Molecular weight: 109596.82 Da Isoelectric Point: 4.8526
>WP_151819900.1 MULTISPECIES: MobH family relaxase [Enterobacteriaceae]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEEPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEEPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 8493 | GenBank | WP_151819909 |
| Name | traC_TMSI_RS27630_pTMSNI47-1 |
UniProt ID | _ |
| Length | 815 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 815 a.a. Molecular weight: 92618.89 Da Isoelectric Point: 5.9222
>WP_151819909.1 type IV secretion system protein TraC [Klebsiella quasipneumoniae]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSQGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRRQAA
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSQGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRRQAA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 43638..66365
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| TMSI_RS31340 | 41389..41472 | + | 84 | Protein_59 | hypothetical protein | - |
| TMSI_RS31155 | 41776..43086 | + | 1311 | Protein_60 | conjugative transfer system coupling protein TraD | - |
| TMSI_RS27560 (TMSI_52960) | 43136..43681 | + | 546 | WP_227549712 | hypothetical protein | - |
| TMSI_RS27565 (TMSI_52970) | 43638..44267 | + | 630 | WP_000743450 | DUF4400 domain-containing protein | tfc7 |
| TMSI_RS27570 | 44277..44723 | + | 447 | WP_151819903 | hypothetical protein | - |
| TMSI_RS27575 (TMSI_52990) | 44733..45110 | + | 378 | WP_151819904 | hypothetical protein | - |
| TMSI_RS27580 (TMSI_53000) | 45110..45772 | + | 663 | WP_151819905 | hypothetical protein | - |
| TMSI_RS27585 (TMSI_53010) | 46109..46474 | + | 366 | WP_227549713 | hypothetical protein | - |
| TMSI_RS27590 (TMSI_53020) | 46619..46900 | + | 282 | WP_000805625 | type IV conjugative transfer system protein TraL | traL |
| TMSI_RS27595 (TMSI_53030) | 46897..47523 | + | 627 | WP_001049716 | TraE/TraK family type IV conjugative transfer system protein | traE |
| TMSI_RS27600 (TMSI_53040) | 47507..48424 | + | 918 | WP_000794249 | type-F conjugative transfer system secretin TraK | traK |
| TMSI_RS27605 (TMSI_53050) | 48424..49737 | + | 1314 | WP_164597232 | TraB/VirB10 family protein | traB |
| TMSI_RS27610 (TMSI_53060) | 49734..50312 | + | 579 | WP_000793435 | type IV conjugative transfer system lipoprotein TraV | traV |
| TMSI_RS27615 (TMSI_53070) | 50325..50708 | + | 384 | WP_227549714 | TraA family conjugative transfer protein | - |
| TMSI_RS27620 (TMSI_53080) | 50909..56395 | + | 5487 | WP_151819907 | Ig-like domain-containing protein | - |
| TMSI_RS27625 (TMSI_53090) | 56544..57251 | + | 708 | WP_151819908 | DsbC family protein | - |
| TMSI_RS27630 (TMSI_53100) | 57248..59695 | + | 2448 | WP_151819909 | type IV secretion system protein TraC | virb4 |
| TMSI_RS27635 (TMSI_53110) | 59710..60027 | + | 318 | WP_000351984 | hypothetical protein | - |
| TMSI_RS27640 (TMSI_53120) | 60024..60554 | + | 531 | WP_001010740 | S26 family signal peptidase | - |
| TMSI_RS27645 (TMSI_53130) | 60517..61782 | + | 1266 | WP_001447719 | TrbC family F-type conjugative pilus assembly protein | traW |
| TMSI_RS27650 (TMSI_53140) | 61779..62450 | + | 672 | WP_000575345 | EAL domain-containing protein | - |
| TMSI_RS27655 | 62450..63454 | + | 1005 | WP_005507569 | TraU family protein | traU |
| TMSI_RS27660 (TMSI_53160) | 63570..66365 | + | 2796 | WP_001256484 | conjugal transfer mating pair stabilization protein TraN | traN |
| TMSI_RS27665 (TMSI_53180) | 66469..67668 | + | 1200 | WP_064152978 | ISL3 family transposase | - |
| TMSI_RS27670 (TMSI_53190) | 67713..68573 | - | 861 | WP_000709517 | hypothetical protein | - |
| TMSI_RS27675 (TMSI_53200) | 68696..69337 | - | 642 | WP_000796664 | hypothetical protein | - |
| TMSI_RS27680 (TMSI_53210) | 69632..69952 | + | 321 | WP_000547566 | hypothetical protein | - |
| TMSI_RS27685 (TMSI_53220) | 70256..70441 | + | 186 | WP_001186917 | hypothetical protein | - |
Host bacterium
| ID | 11909 | GenBank | NZ_AP019688 |
| Plasmid name | pTMSNI47-1 | Incompatibility group | IncA/C2 |
| Plasmid size | 185311 bp | Coordinate of oriT [Strand] | 43137..43287 [+] |
| Host baterium | Klebsiella quasipneumoniae strain SNI47 |
Cargo genes
| Drug resistance gene | sul1, qacE, ant(3'')-Ia, blaOXA-10, cmlA1, ARR-3, dfrA14, blaCTX-M-2, qnrB4, blaDHA-1, blaKHM-1 |
| Virulence gene | - |
| Metal resistance gene | merE, merD, merA, merP, merT, merR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |