Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 111460
• Name: oriT_pBE5_4
• Organism: Enterococcus faecalis strain BE5
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP110078 (1233..1282 [-], 50 nt)
• oriT length: 50 nt
• IRs (inverted repeats): _
• Location of nic site: 34..35
• Conserved sequence flanking the nic site: GCTTGCAAAA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 50 nt

>oriT_pBE5_4
AATATCGCAACATGGTACCATGTTGCTCCGCTTGCAAAAAGAAAGCCTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_OLM06_RS15020_pBE5_4

• ID: 7453
• GenBank: WP_010717414
• Name: mobV_OLM06_RS15020_pBE5_4
• UniProt ID: Q9ZEH9
• Length: 559 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 559 a.a.
• Molecular weight: 65102.51 Da
• Isoelectric Point: 7.9635

>WP_010717414.1 MULTISPECIES: MobV family relaxase [Bacteria]
MSKIVARMEKMKDGNLSGIQRHNQRETNNHSNPDIDIEKSHLNYDLVNPGSINYREKIKQIIESQRISKR
AVRKDAVLVNEWIITSDTAFFQENTDTQAFFTDVVAYFSDRCGRQNVAYATVHLDETTPHMHLGIVPMYE
GRLSSKQVFSRQNLLEIQEELPTYLQERGYAIERGLRGSPQKHLSVKEYKDVQKELQVSKQELANQEQIL
AEKERELHSYEQAIFRIDRLLEEKNEEVKGKVQAMNQLETKRNEIINSLQTTVLPDLANVKEKKFSLNGL
KYVLSASDFSAIKSITKDFETLRTQNKHLKIENNNLAQKNKELTQVNHEQELQITELAYELTLKKEQLTA
FEEKQLNYDQSEQIKQELDTTTQTVSVLETENKRLKDKITDLTYDFSELNEKYQEIKLKFDGLTTYLHER
LGHAKETILFTIQQGVKYLQNAPKLKKSLERYSKDRTYLIDEKGTLFLPPDTGSKGVTMSLNPHFYPLKA
PFYNRETGCYEFVGEYMANGRSRTNTLQLSEEQVLEKKAFTFEQSTDKKAWQQSKKYSRDMSKGRGLSR

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (2-191)

Protein structure

• AlphaFold DB: Q9ZEH9

Host bacterium

• ID: 11895
• GenBank: NZ_CP110078
• Plasmid name: pBE5_4
• Incompatibility group: -
• Plasmid size: 5143 bp
• Coordinate of oriT [Strand]: 1233..1282 [-]
• Host baterium: Enterococcus faecalis strain BE5

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -