Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 111293 |
| Name | oriT_pEh19_OXA48  |
| Organism | Enterobacter hormaechei strain Ehh_19 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP126794 (40138..40243 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_pEh19_OXA48
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 7367 | GenBank | WP_004187323 |
| Name | traI_QPR73_RS23560_pEh19_OXA48 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 8402 | GenBank | WP_004206885 |
| Name | t4cp2_QPR73_RS23265_pEh19_OXA48 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 44140..63105
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| QPR73_RS23550 (QPR73_23515) | 39605..40000 | - | 396 | WP_019725163 | hypothetical protein | - |
| QPR73_RS23555 (QPR73_23520) | 40035..40562 | + | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| QPR73_RS23560 (QPR73_23525) | 40549..42528 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| QPR73_RS23565 (QPR73_23530) | 42542..43042 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| QPR73_RS23570 (QPR73_23535) | 43039..43386 | + | 348 | Protein_63 | type IV secretory system conjugative DNA transfer family protein | - |
| QPR73_RS23575 (QPR73_23540) | 43441..44138 | + | 698 | WP_095033700 | IS1-like element IS1B family transposase | - |
| QPR73_RS23580 (QPR73_23545) | 44140..44595 | + | 456 | WP_311903249 | type IV secretory system conjugative DNA transfer family protein | traI |
| QPR73_RS23585 (QPR73_23550) | 44606..45769 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| QPR73_RS23590 (QPR73_23555) | 45759..46019 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| QPR73_RS23595 (QPR73_23560) | 46044..49481 | + | 3438 | WP_015586048 | LPD7 domain-containing protein | - |
| QPR73_RS23600 (QPR73_23565) | 49447..49959 | + | 513 | WP_011091071 | hypothetical protein | traL |
| QPR73_RS23605 (QPR73_23570) | 49960..50172 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| QPR73_RS23610 (QPR73_23575) | 50144..50554 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| QPR73_RS23615 (QPR73_23580) | 50622..51335 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| QPR73_RS23620 (QPR73_23585) | 51344..52495 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| QPR73_RS23625 (QPR73_23590) | 52507..53856 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| QPR73_RS23630 (QPR73_23595) | 53868..54572 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| QPR73_RS23635 (QPR73_23600) | 54596..55126 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| QPR73_RS23640 (QPR73_23605) | 55143..55532 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| QPR73_RS23645 (QPR73_23610) | 55578..56072 | + | 495 | WP_004187480 | hypothetical protein | - |
| QPR73_RS23650 (QPR73_23615) | 56069..59119 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| QPR73_RS23655 (QPR73_23620) | 59116..60324 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| QPR73_RS23660 (QPR73_23625) | 60321..60917 | + | 597 | WP_015060003 | hypothetical protein | - |
| QPR73_RS23665 (QPR73_23630) | 60910..63105 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| QPR73_RS23670 (QPR73_23635) | 63107..63760 | + | 654 | WP_015060005 | hypothetical protein | - |
| QPR73_RS23675 (QPR73_23640) | 63841..64071 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 11728 | GenBank | NZ_CP126794 |
| Plasmid name | pEh19_OXA48 | Incompatibility group | IncL/M |
| Plasmid size | 64366 bp | Coordinate of oriT [Strand] | 40138..40243 [+] |
| Host baterium | Enterobacter hormaechei strain Ehh_19 |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |