Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 111189 |
| Name | oriT_pSL267  |
| Organism | Serratia marcescens strain Serratia marcescens SL267 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_AP024917 (57780..57885 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_pSL267
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 7325 | GenBank | WP_004187323 |
| Name | traI_LPG96_RS24630_pSL267 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 8349 | GenBank | WP_004206885 |
| Name | t4cp2_LPG96_RS24375_pSL267 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 60681..79877
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LPG96_RS24610 (SL267_48070) | 55766..56077 | + | 312 | WP_053919080 | hypothetical protein | - |
| LPG96_RS24615 (SL267_48080) | 56210..56746 | + | 537 | WP_011091066 | hypothetical protein | - |
| LPG96_RS24620 (SL267_48100) | 57247..57642 | - | 396 | WP_170949733 | MobC | - |
| LPG96_RS24625 | 57677..58204 | + | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| LPG96_RS24630 (SL267_48120) | 58191..60170 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| LPG96_RS24635 (SL267_48130) | 60184..60684 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| LPG96_RS24640 (SL267_48140) | 60681..61460 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| LPG96_RS24645 (SL267_48150) | 61471..62634 | + | 1164 | WP_077138898 | plasmid transfer ATPase TraJ | virB11 |
| LPG96_RS24650 (SL267_48160) | 62624..62884 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| LPG96_RS24655 (SL267_48170) | 62909..66214 | + | 3306 | WP_227649763 | LPD7 domain-containing protein | - |
| LPG96_RS24660 (SL267_48180) | 66180..66692 | + | 513 | WP_011091071 | hypothetical protein | traL |
| LPG96_RS24665 (SL267_48190) | 66693..66905 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| LPG96_RS24670 (SL267_48200) | 66877..67287 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| LPG96_RS24675 (SL267_48210) | 67355..68068 | + | 714 | WP_223294958 | DotI/IcmL family type IV secretion protein | traM |
| LPG96_RS24680 (SL267_48220) | 68077..69228 | + | 1152 | WP_015062843 | DotH/IcmK family type IV secretion protein | traN |
| LPG96_RS24685 (SL267_48230) | 69240..70589 | + | 1350 | WP_004206932 | conjugal transfer protein TraO | traO |
| LPG96_RS24690 (SL267_48240) | 70601..71305 | + | 705 | WP_004206933 | conjugal transfer protein TraP | traP |
| LPG96_RS24695 (SL267_48250) | 71329..71859 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| LPG96_RS24700 (SL267_48260) | 71876..72265 | + | 390 | WP_011154472 | DUF6750 family protein | traR |
| LPG96_RS24705 (SL267_48270) | 72311..72805 | + | 495 | WP_004187480 | hypothetical protein | - |
| LPG96_RS24710 (SL267_48280) | 72802..75852 | + | 3051 | WP_096159684 | conjugal transfer protein | traU |
| LPG96_RS24715 (SL267_48290) | 75849..77057 | + | 1209 | WP_004187486 | conjugal transfer protein TraW | traW |
| LPG96_RS24720 (SL267_48300) | 77054..77704 | + | 651 | WP_096159686 | TraX-like protein | - |
| LPG96_RS24725 (SL267_48310) | 77697..79877 | + | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| LPG96_RS24730 (SL267_48320) | 79880..80533 | + | 654 | WP_004206935 | hypothetical protein | - |
| LPG96_RS24735 (SL267_48330) | 80607..80837 | + | 231 | WP_004187496 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 11624 | GenBank | NZ_AP024917 |
| Plasmid name | pSL267 | Incompatibility group | IncL/M |
| Plasmid size | 81133 bp | Coordinate of oriT [Strand] | 57780..57885 [+] |
| Host baterium | Serratia marcescens strain Serratia marcescens SL267 |
Cargo genes
| Drug resistance gene | blaTEM-1B, blaIMP-1, aac(6')-IIc, qacE, sul1 |
| Virulence gene | - |
| Metal resistance gene | merE, merD, merA, merC, merP, merT, merR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |