Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   111101
Name   oriT_pSa42-91k in_silico
Organism   Salmonella enterica subsp. enterica serovar Meleagridis strain Sa42
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MW567497 (36351..36439 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pSa42-91k
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   7286 GenBank   WP_001306778
Name   nikB_KV197_RS00260_pSa42-91k insolico UniProt ID   Q79VV7
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104010.41 Da        Isoelectric Point: 7.4265

>WP_001306778.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB Q79VV7


Auxiliary protein

ID   4051 GenBank   WP_001283947
Name   WP_001283947_pSa42-91k insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   8300 GenBank   WP_021497839
Name   trbC_KV197_RS00265_pSa42-91k insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86967.19 Da        Isoelectric Point: 7.0662

>WP_021497839.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLKCADPSQDRMIKRSLFSFWPTLFQYEVILESPANGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 41817..79771

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KV197_RS00265 (CHIMEGLD_00049) 39533..41824 - 2292 WP_021497839 F-type conjugative transfer protein TrbC -
KV197_RS00270 (CHIMEGLD_00050) 41817..42887 - 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
KV197_RS00275 (CHIMEGLD_00051) 42906..44114 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
KV197_RS00280 (CHIMEGLD_00052) 44406..44558 + 153 WP_001387489 Hok/Gef family protein -
KV197_RS00285 (CHIMEGLD_00053) 44630..44881 - 252 WP_001291964 hypothetical protein -
KV197_RS00520 45380..45475 + 96 WP_001303310 DinQ-like type I toxin DqlB -
KV197_RS00290 45540..45824 - 285 WP_014640317 hypothetical protein -
KV197_RS00295 (CHIMEGLD_00055) 46054..46257 + 204 WP_001383957 HEAT repeat domain-containing protein -
KV197_RS00300 (CHIMEGLD_00056) 46329..46991 - 663 WP_000644792 plasmid IncI1-type surface exclusion protein ExcA -
KV197_RS00305 (CHIMEGLD_00057) 47056..49224 - 2169 WP_077250315 DotA/TraY family protein traY
KV197_RS00310 (CHIMEGLD_00058) 49321..49905 - 585 WP_001037996 IncI1-type conjugal transfer protein TraX -
KV197_RS00315 (CHIMEGLD_00059) 49934..51136 - 1203 WP_001189156 IncI1-type conjugal transfer protein TraW traW
KV197_RS00320 (CHIMEGLD_00060) 51103..51717 - 615 WP_000337399 IncI1-type conjugal transfer protein TraV traV
KV197_RS00325 (CHIMEGLD_00061) 51717..54761 - 3045 WP_001024776 IncI1-type conjugal transfer protein TraU traU
KV197_RS00330 (CHIMEGLD_00062) 54851..55651 - 801 WP_001164792 IncI1-type conjugal transfer protein TraT traT
KV197_RS00335 (CHIMEGLD_00063) 55635..55823 - 189 WP_001277255 putative conjugal transfer protein TraS -
KV197_RS00340 (CHIMEGLD_00064) 55887..56291 - 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
KV197_RS00345 (CHIMEGLD_00065) 56342..56869 - 528 WP_001055569 conjugal transfer protein TraQ traQ
KV197_RS00350 (CHIMEGLD_00066) 56869..57573 - 705 WP_023155244 IncI1-type conjugal transfer protein TraP traP
KV197_RS00355 (CHIMEGLD_00067) 57573..58862 - 1290 WP_001271994 conjugal transfer protein TraO traO
KV197_RS00360 (CHIMEGLD_00068) 58865..59848 - 984 WP_021513963 IncI1-type conjugal transfer protein TraN traN
KV197_RS00365 (CHIMEGLD_00069) 59859..60551 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
KV197_RS00370 (CHIMEGLD_00070) 60548..60895 - 348 WP_001055900 conjugal transfer protein traL
KV197_RS00375 (CHIMEGLD_00071) 60913..64680 - 3768 WP_006855639 LPD7 domain-containing protein -
KV197_RS00380 (CHIMEGLD_00072) 64770..65321 - 552 WP_000014584 phospholipase D family protein -
KV197_RS00385 (CHIMEGLD_00073) 65336..65626 - 291 WP_001299214 hypothetical protein traK
KV197_RS00390 (CHIMEGLD_00074) 65623..66771 - 1149 WP_001024976 plasmid transfer ATPase TraJ virB11
KV197_RS00395 (CHIMEGLD_00075) 66768..67586 - 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
KV197_RS00400 (CHIMEGLD_00076) 67583..68041 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
KV197_RS00405 (CHIMEGLD_00077) 68436..69020 - 585 WP_124770274 histidine phosphatase family protein -
KV197_RS00410 (CHIMEGLD_00078) 69080..70282 - 1203 WP_000976357 conjugal transfer protein TraF -
KV197_RS00415 (CHIMEGLD_00079) 70368..71192 - 825 WP_015058912 conjugal transfer protein TraE traE
KV197_RS00420 (CHIMEGLD_00080) 71343..72497 - 1155 WP_001139955 site-specific integrase -
KV197_RS00425 73157..73312 + 156 WP_001302705 hypothetical protein -
KV197_RS00430 (CHIMEGLD_00081) 74187..75290 - 1104 Protein_86 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
KV197_RS00435 (CHIMEGLD_00082) 75278..75934 - 657 WP_001193551 A24 family peptidase -
KV197_RS00440 (CHIMEGLD_00083) 75919..76479 - 561 WP_000014005 lytic transglycosylase domain-containing protein virB1
KV197_RS00445 (CHIMEGLD_00084) 76489..77103 - 615 WP_000908228 type 4 pilus major pilin -
KV197_RS00450 (CHIMEGLD_00085) 77120..78205 - 1086 WP_001208803 type II secretion system F family protein -
KV197_RS00455 (CHIMEGLD_00086) 78218..79771 - 1554 WP_000362204 ATPase, T2SS/T4P/T4SS family virB11
KV197_RS00460 (CHIMEGLD_00087) 79782..80234 - 453 WP_001247333 type IV pilus biogenesis protein PilP -
KV197_RS00465 (CHIMEGLD_00088) 80221..81516 - 1296 WP_000752776 type 4b pilus protein PilO2 -
KV197_RS00470 (CHIMEGLD_00089) 81509..83191 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
KV197_RS00475 (CHIMEGLD_00090) 83205..83642 - 438 WP_000539811 type IV pilus biogenesis protein PilM -
KV197_RS00480 (CHIMEGLD_00091) 83642..84709 - 1068 WP_021513968 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   11536 GenBank   NZ_MW567497
Plasmid name   pSa42-91k Incompatibility group   IncI1
Plasmid size   91229 bp Coordinate of oriT [Strand]   36351..36439 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Meleagridis strain Sa42

Cargo genes

Drug resistance gene   blaCTX-M-24
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -