Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   111009
Name   oriT_CYX|unnamed2 in_silico
Organism   Salmonella sp. strain CYX
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OP927720 (19205..19811 [+], 607 nt)
oriT length   607 nt
IRs (inverted repeats)      590..595, 600..605  (ATGCCC..GGGCAT)
 517..523, 529..535  (CCTCCCG..CGGGAGG)
 453..458, 462..467  (GTTCGC..GCGAAC)
 196..202, 204..210  (TCAATTC..GAATTGA)
 175..180, 187..192  (AAATCA..TGATTT)
 103..108, 120..125  (GCTAAA..TTTAGC)
 79..84, 93..98  (GCTTTA..TAAAGC)
 29..37, 46..54  (TTTTGATAA..TTATCAAAA)
Location of nic site      313..314
Conserved sequence flanking the
  nic site  
 
 TCCTGCATCG
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 607 nt

>oriT_CYX|unnamed2
CTTTGTTTACCTGTTAAGTACATCGTTGTTTTGATAAAATCATTATTATCAAAAAACGTATTTATACCCTTACTGCCAGCTTTACTTTTTAATAAAGCATTTGCTAAAAGTTTCATGCCTTTAGCTACTGTTAAAGCTGGTTCATCTGGATAAAGAGTTTTAAGGCAATCCAGCAAATCATCATGCTGATTTTCATCAATTCTGAATTGAACTTTTTTCATAAAATTCTCCGACAAGAACCGACTGTAGGTCACCGGGCAAACGTTGCGGAATGGCGTTAGAGACGTCATTTTGCGGCGTTTGCCCTATCCTGCATCGCAGTGGATCTGGCACCGCTCATAAATTGTGCTTGAGCATCAACAAAAATGCAAAAAGGCCGATGTTGTCATGGCTGAACATACGTGCGTCTGCGACCGGAAACTCGATCAATGCGGTAAGGACAGTGCCGTCAGGTTCGCTCCGCGAACGGTCTGGCAGGAAGCGCAAGCGCAGTCCCGCGCAATATATCAGAAAGCACCTCCCGAAATACGGGAGGGCTTCGGCTATTCAGCTAAGGTTGCTCTGTTCAGGTTGCTGAGTGTAGCCTTCCATGCCCTGACGGGCATCA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   7242 GenBank   WP_000539539
Name   mobP1_QZJ71_RS00100_CYX|unnamed2 insolico UniProt ID   A0A748H4F7
Length   388 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 388 a.a.        Molecular weight: 44419.77 Da        Isoelectric Point: 10.5368

>WP_000539539.1 MULTISPECIES: MobP1 family relaxase [Enterobacteriaceae]
MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAVTRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKAKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GGLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFWGL

  Protein domains


Predicted by InterproScan.

(70-227)


  Protein structure


Source ID Structure
AlphaFold DB A0A748H4F7


Host bacterium


ID   11444 GenBank   NZ_OP927720
Plasmid name   CYX|unnamed2 Incompatibility group   IncX1
Plasmid size   24484 bp Coordinate of oriT [Strand]   19205..19811 [+]
Host baterium   Salmonella sp. strain CYX

Cargo genes


Drug resistance gene   blaTEM-1B, aph(6)-Id, aph(3'')-Ib, sul2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -