Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   110997
Name   oriT_pAUSMDU00010535_03 in_silico
Organism   Shigella flexneri 2a strain AUSMDU00010535
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP045944 (1389..1563 [+], 175 nt)
oriT length   175 nt
IRs (inverted repeats)      101..106, 120..125  (ACAATC..GATTGT)
Location of nic site      65..66
Conserved sequence flanking the
  nic site  
 
 ACTGGCATAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 175 nt

>oriT_pAUSMDU00010535_03
GTGACTCCGTGCGCGGTGAAAAATCGTATTTTAGCGCGTCACTGGTAGTTGCAAAACTGAACTGGCATAATGCACGGCACATCACGAAGTGCGCACTTATACAATCTTCACTTCGTTTCGATTGTGTGCGGTCTGCGACGCTAAAAGAAAACGGCAAAAAGGCATTACGGCAGAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   7235 GenBank   WP_154074701
Name   mobQ_GJE14_RS25595_pAUSMDU00010535_03 insolico UniProt ID   _
Length   739 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 739 a.a.        Molecular weight: 84317.43 Da        Isoelectric Point: 8.3340

>WP_154074701.1 MULTISPECIES: MobQ family relaxase [Enterobacteriaceae]
MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTRKRGVDDAVILTPVNAPSWCGDRSVLW
NAVEKAEQRRNSQLAREIELAIPREISREAAREAVLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTT
RAVGETGFAGKVRDWNDRALAETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAM
EKRGMETERGEQNRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLR
EFIATLPQECGNAWEMTPEFVAMNGKANDIKREGNALVKERDILEKEMTGLRKARPVASILSELPLMTWA
EPEYRKRQLRFWKLEKQIESLRRTYRAVKERDIPARRQAFETQWNTWISPGMAELKEKLSAREAERRREE
AEAEARRKEQEYDARLKHHDNHRLSRETALAGVITGLGRAREPGTGMITRYMILSNRAGEFTVWGDELAD
YPQSVHDQVNVYLSPGGAVMVTDIHSGLSESHETLARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPH
HWPDNFYFSDDRRPAASPLPSAHRVDVTAYAAPEQLMPVVFSTERNSRTLNLLLCKGPEEMLVGFVRLED
ELRPVLALPSPDYSHLMVSIITENGIHLAGYGEAINRDADTPYPPEPKLMQFRLKGCQDTLFAAINKPEE
MPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHYRGMSM

  Protein domains


Predicted by InterproScan.

(18-213)


  Protein structure



No available structure.




Host bacterium


ID   11432 GenBank   NZ_CP045944
Plasmid name   pAUSMDU00010535_03 Incompatibility group   Col156
Plasmid size   4692 bp Coordinate of oriT [Strand]   1389..1563 [+]
Host baterium   Shigella flexneri 2a strain AUSMDU00010535

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -