Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 110983
• Name: oriT_pVPRE25a
• Organism: Enterococcus faecalis strain RE25
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP128462 (1233..1282 [-], 50 nt)
• oriT length: 50 nt
• IRs (inverted repeats): _
• Location of nic site: 34..35
• Conserved sequence flanking the nic site: GCTTGCAAAA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 50 nt

>oriT_pVPRE25a
AATATCGCAACATGGTACCATGTTGCTCCGCTTGCAAAAAGAAAGCCTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_QSV41_RS00040_pVPRE25a

• ID: 7229
• GenBank: WP_289690431
• Name: mobV_QSV41_RS00040_pVPRE25a
• UniProt ID: _
• Length: 559 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 559 a.a.
• Molecular weight: 65101.57 Da
• Isoelectric Point: 8.6009

>WP_289690431.1 MobV family relaxase [Enterococcus faecalis]
MSKIVARMEKMKDGNLSGIQRHNQRETNNHSNPDIDIEKSHLNYDLVNPGSINYREKIKQIIESQRISKR
AVRKDAVLVNEWIITSDTAFFQKNTDTQAFFTDVVAYFSDRCGRQNVAYATVHLDETTPHMHLGIVPMYE
GRLSSKQVFSRQNLLEIQEELPTYLQERGYAIERGLRGSPQKHLSVKEYKDVQKELQVSKQELANQEQIL
AEKERELHSYEQAIFRIDRLLEEKNEEVKGKVQAMNQLETKRNEIINSLQTTVLPDLANVKEKKFSLNGL
KYVLSASDFSAIKSITKDFETLRTQNKHLKIENNNLAQKNKELTQVNHEQELQITELAYELTLKKEQLTA
FEEKQLNYDQSEQIKQELDTTTQTVSVLETENKRLKDKITDLTYDFSELNEKYQEIKLKFDGLTTYLHER
LGHAKETILFTIQQGVKYLQNAPKLKKSLERYSKDRTYLIDEKGTLFLPPDTGSKGVTMSLNPHFYPLKA
PFYNRETGCYEFVGEYMANGRSRTNTLQLSEEQVLEKKAFTFEQSTDKKAWQQSKKYSRDMSKGRGLSR

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (2-191)

Protein structure

No available structure.

Host bacterium

• ID: 11418
• GenBank: NZ_CP128462
• Plasmid name: pVPRE25a
• Incompatibility group: -
• Plasmid size: 5141 bp
• Coordinate of oriT [Strand]: 1233..1282 [-]
• Host baterium: Enterococcus faecalis strain RE25

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -