Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   110906
Name   oriT_CGMCC 2108|unnamed2 in_silico
Organism   Bacillus subtilis subsp. natto strain CGMCC 2108
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP014473 (27531..27892 [+], 362 nt)
oriT length   362 nt
IRs (inverted repeats)      188..193, 198..203  (CACCAT..ATGGTG)
 171..178, 180..187  (AAACCGCA..TGCGGTTT)
 116..122, 130..136  (ACATAAA..TTTATGT)
 84..89, 101..106  (ATTTAG..CTAAAT)
 84..89, 92..97  (ATTTAG..CTAAAT)
 62..67, 75..80  (CATAAA..TTTATG)
 48..54, 65..71  (TTATTTT..AAAATAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 362 nt

>oriT_CGMCC 2108|unnamed2
AAAGAGCAATCTCGTCATCGAAGACTAAATTTCTGTATGGAAAACAGTTATTTTTCGTGTGCATAAAATAAAGATTTATGTGCATTTAGTTCTAAATCACCTAAATAATGGTTGAACATAAATGTTTTTTTTATGTGCATTCAGCAATAAATCTGGTACCACGAAAAAACAAACCGCACTGCGGTTTCACCATGCAAATGGTGCCAGTTTCCCCTTATGCTCTTTCCTTTTCCACCCCCTCCTTCCTTCGGAATCGGGGGCCGGCTTTTTGCTGCCGCAAAAAGCCTTGGAAAAAGAAACACTTATTTGAACAGATCGTTGCAAAGTAATGTGCAGAAATCGATGAAAAGGAGCGTTAACAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   7203 GenBank   WP_013603221
Name   mobP2_AWV81_RS22185_CGMCC 2108|unnamed2 insolico UniProt ID   E9RJ23
Length   410 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 48596.08 Da        Isoelectric Point: 10.0206

>WP_013603221.1 MULTISPECIES: MobP2 family relaxase [Bacillaceae]
MDSPGVVLVSKYVSGKSTKFSKYVNYINRDEAVRTEKFQTYNVNKLDGYNQYMGNPEKSSGIFTQHKDSL
SPVEKNQLKEIFRQAQKNDSVMWQDVISFDNKWLEERGIYNSQTGWVNEGAIQNSIRKGMEVLLREEQLE
QSGVWSAAIHYNTDNIHVHIALVEPNPTKEYGVFTNKKTGEVYQARRGNRKLKTLDKMKSKVANTLMDRD
KELSKISQLIHDRIAPKGLKFQPRLDTYMTKMYNHIYENLPEDMRLWKYNNNALNNIRPEIDSVITMYIQ
KYHPEDYKELDQSLKEEMEFRKSVYGDGPKQVERYKEYRKNKHKELYTKLGNSMLKEMAEIRRRESQSKQ
MNRSGTYAPSSVSNRQWDTKGRRIKRSDINKIKHALDKDYQSMKNMRKYQQMQYEMEQSR

  Protein domains


Predicted by InterproScan.

MobL relaxases (MobL)

(3-389)


  Protein structure


Source ID Structure
AlphaFold DB E9RJ23


Host bacterium

ID   11341 GenBank   NZ_CP014473
Plasmid name   CGMCC 2108|unnamed2 Incompatibility group   -
Plasmid size   65774 bp Coordinate of oriT [Strand]   27531..27892 [+]
Host baterium   Bacillus subtilis subsp. natto strain CGMCC 2108

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -