Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   110634
Name   oriT_pSkuCCBAU71714b in_silico
Organism   Sinorhizobium kummerowiae strain CCBAU 71714
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP120364 (1455449..1455477 [+], 29 nt)
oriT length   29 nt
IRs (inverted repeats)      14..19, 24..29  (CGTCGC..GCGACG)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 29 nt

>oriT_pSkuCCBAU71714b
AGGGCGCAATATACGTCGCTGGCGCGACG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   7074 GenBank   WP_284718328
Name   traA_PZL22_RS06670_pSkuCCBAU71714b insolico UniProt ID   _
Length   1539 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1539 a.a.        Molecular weight: 169883.60 Da        Isoelectric Point: 9.4589

>WP_284718328.1 Ti-type conjugative transfer relaxase TraA [Sinorhizobium kummerowiae]
MAIMFVRAQVIGRGAGRSIVSAAAYRHRTRMIDEQAGTSFSYRGGASELVHEALALPDDIPAWLKAAIDG
QSVAKASEALWNAVEAFETRADAQLARELIIALPEELTRAENIALVREFVRDNLTSKGMVADWVYHDKDG
NPHIHLMTALRPLTEEGFGPKKVAVTGEDGEPLRVITPDRPTGKIVYKLWAGDKETIKAWKIAWAETANR
HLALAGHEIRLDGRSYAEQGLDGIAQKHLGPEKAALARKGIAMYFAPADLARRQEMADRLLAEPELLLKQ
LGNERSTFDERDIARALHRYVDDPVDFANIRARLMASDDLVLLKPQQVDAETGKAKQPAVFTTREMLRIE
YDMARSAEVLSKRKGFGVSNARVAAAVRSIETADTEKPFRLDPEQVDAVHHVTRDNAIAAVVGLAGAGKS
TLLAAARLAWEGDGRRVIGAALAGKAAEGLEDSSGIRSRTLASWELAWESGRETLQRGDVLVIDEAGMVS
SQQMARILKAVEDAGAKAVLVGDAMQLQPIEAGAAFRAISERIGFAELAGVRRQRDAWARDASRLFARGK
IEEGLDAYAQQGRIVETETRAEIVDRIVADWANARRDLLQKSADGEHPGRLRGDELLVLAHTNDDVRKLN
EALRNVMIGEGALAGAREFQTARGLREFAAGDRIIFLENARFVEPRARRLGPQYVKNGMLGTVVSTGDRR
GDTLLSVRLDSGRDVVISQDSYRNVDHGYAATIHKSQGSTVDRTFVLATGMMDQHLTYVAMTRHRDRADL
YAAKEDFEAKPEWGRKPRVDHAAGVTGELVKEGMAKFRPNDEDADESPYADIRTDDGTVHRLWGVSLPKA
LKDAGVAEGDTITLRKDGVERVKVQVPIVDEQTGEKRFEERQVDRNVWSASQLETAAARRERIERESHRP
QLFAALVERLSRSGAKTTTLDFEDEAGYQAQARDFARRRGLYHLSLVAAGVEEEVLRRWAGIAEKRELVA
KLWERASVALGFAIERERRVSYNEERTETLSTGIPLSGKHLIPPTTTFSRSVAEDARLAQLSSERWKERE
AILHPVLAKIYRDPDGALSALNALASDAAIEPRKLADDLGKAPDRLGRLRGSELVVDGRAARDERTAATV
ALSELLPLARAHATEFRRNAERFGIREQQRRAHMSLSVPALSKTAMARLVEIEAVRKQGGDDAFRTAFAF
AVEDRLLVQEVKGVNEALTARFGWSAFTAKADVIAERNIAERMPEDLAPERREKLTRLFAVIRRFAEEQH
LAERQDRSKIVAGASVELGKETFAVLPMLAAVTEFKTPVDEEARTRALSAAHYRHHRVAIAETAKRIWRD
PADAIGKIEDLIVKGFAGERIAAAVSNDPAAYGALRGSDRIMDKLLAAGRERRDALQALPEAASRIRSLG
ASYASALDAETRSITEERRRMAVAIPGLSPAADDALRRLAAQIKNKDSSLAVVAGALAPAVAREFAKVSR
ALDERFGRNAILRGETDVINRVSPAQRRAFEAMRDRLQVLQQTVRVQSSEKIVSERRQRAINQSRGIRM

  Protein domains


Predicted by InterproScan.

(17-249)

(1053-1125)

(1310-1400)

(391-578)


  Protein structure



No available structure.




T4CP


ID   8022 GenBank   WP_033048650
Name   tcpA_PZL22_RS03545_pSkuCCBAU71714b insolico UniProt ID   _
Length   947 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 947 a.a.        Molecular weight: 101739.54 Da        Isoelectric Point: 4.5726

>WP_033048650.1 MULTISPECIES: DNA translocase FtsK [Sinorhizobium]
MRIPRTNFSPAALYDANHADDYEDPHPAAQGAAHVPAAPEAAAHDHLFEAPEAPRRAPGHRDEETGYAGR
AARLYGHGSAYAPAEVTVSTRDPLPTLAEITGLSGEPGWESHFFLSPNVRFTRTPERELMKRHPPAPEEN
RAEADEAAAEASDPETVMDVVPAGPAPSVVETELPSYSPSELLRVLTQQLPSWSAARSQAPEASVTKPAI
TESVAVAEEEPATPETPSALPVTDEVPVVPHALPVANLAPESAAVEEDVPHQTDARLAYLSDFAFFEFMP
LEVAAVPPTVTEPVKDAARIPAPIAAVPKVSPPKIVAAMPVEIRPQPPTAISSLFRVVECRRPEPATAEP
VTAAPQDIAAEPAAAEAAALPAPQFVETPAPALAEPAIVPASEPAPEAPVTRAAITMPAVIQRSSPSLPP
IGALEPLQGGDAYEFPSKELLQEPPQGQGFFMTQEQLEQNAGLLESVLEDFGVKGEIIHVRPGPVVTLYE
FEPAPGVKSSRVIGLADDIARSMSALSARVAVVPGRNVIGIELPNATRETVYFRELIESGDFQKTGCKLA
LCLGKTIGGEPVIAELAKMPHLLVAGTTGSGKSVAINTMILSLLYRLKPEECRLIMVDPKMLELSVYDGI
PHLLTPVVTDPKKAVMALKWAVREMEDRYRKMSRLGVRNIDGYNQRAAAAREKGAPILATVQTGFEKGTG
EPLFEQQEMDLSPMPYIVVIVDEMADLMMVAGKEIEGAIQRLAQMARAAGIHLIMATQRPSVDVITGTIK
ANFPTRISFQVTSKIDSRTILGEQGAEQLLGQGDMLHMAGGGRIARVHGPFVSDQEVEHVVAHLKTQGRP
EYLETVTADEEEEEVEEDQGAVFDKSAIAAEDGNELYDQAVKVVLRDKKCSTSYIQRRLGIGYNRAASLV
ERMEKDGLVGPANHVGKREIIYGNRDNPPKPESDDLD

  Protein domains


Predicted by InterproScan.

(544-762)

(872-931)

(435-535)

  Protein structure



No available structure.




Host bacterium


ID   11069 GenBank   NZ_CP120364
Plasmid name   pSkuCCBAU71714b Incompatibility group   -
Plasmid size   1543136 bp Coordinate of oriT [Strand]   1455449..1455477 [+]
Host baterium   Sinorhizobium kummerowiae strain CCBAU 71714

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   actP
Degradation gene   linJ
Symbiosis gene   -
Anti-CRISPR   AcrIIA7