Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   110324
Name   oriT_pCTX-M_SCLZS19 in_silico
Organism   Enterobacter kobei strain SCLZS19
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP110879 (140032..140285 [+], 254 nt)
oriT length   254 nt
IRs (inverted repeats)      152..157, 159..164  (AAAAGT..ACTTTT)
 121..129, 134..142  (TTAAGGCTT..AAGCCTTAA)
 48..54, 59..65  (GAATTTT..AAAATTC)
Location of nic site      77..78
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 254 nt

>oriT_pCTX-M_SCLZS19
GGATTTAGGTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   6848 GenBank   WP_012477394
Name   mobH_OQE50_RS25930_pCTX-M_SCLZS19 insolico UniProt ID   A0A2Z2E7B6
Length   1050 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1050 a.a.        Molecular weight: 118067.47 Da        Isoelectric Point: 4.6347

>WP_012477394.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDT
VETAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFSQLSVSRPEVG
SCATSSSVHNEKLLSEPSELPELNREQNADPQGTNERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFASTESDQNGEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(108-304)


  Protein structure


Source ID Structure
AlphaFold DB A0A2Z2E7B6


T4CP

ID   7654 GenBank   WP_001284073
Name   traD_OQE50_RS25925_pCTX-M_SCLZS19 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78123.91 Da        Isoelectric Point: 8.0177

>WP_001284073.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(406-609)

AAA-like domain (AAA_10)

(199-249)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 48830..58031

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OQE50_RS25385 (OQE50_25385) 46124..46285 - 162 WP_001371949 hypothetical protein -
OQE50_RS27715 48830..49477 - 648 WP_276182554 hypothetical protein virb4
OQE50_RS25390 (OQE50_25390) 49417..51510 - 2094 WP_281040192 TraC family protein virb4
OQE50_RS25395 (OQE50_25395) 51519..52469 - 951 WP_001022588 IncHI-type conjugal transfer lipoprotein TrhV traV
OQE50_RS25400 (OQE50_25400) 52479..53231 - 753 WP_182911723 protein-disulfide isomerase HtdT -
OQE50_RS25405 (OQE50_25405) 53350..53772 - 423 WP_000251249 hypothetical protein -
OQE50_RS25410 (OQE50_25410) 53839..55194 - 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
OQE50_RS25415 (OQE50_25415) 55184..55645 - 462 WP_000521242 plasmid transfer protein HtdO -
OQE50_RS25420 (OQE50_25420) 55647..56870 - 1224 WP_111962809 type-F conjugative transfer system secretin TraK traK
OQE50_RS25425 (OQE50_25425) 56917..57051 - 135 WP_265177437 hypothetical protein -
OQE50_RS25430 (OQE50_25430) 57105..57347 - 243 WP_251077271 TraE/TraK family type IV conjugative transfer system protein traE
OQE50_RS25435 (OQE50_25435) 57424..57702 - 279 WP_249528914 hypothetical protein traE
OQE50_RS25440 (OQE50_25440) 57714..58031 - 318 WP_000043358 type IV conjugative transfer system protein TraL traL
OQE50_RS25445 (OQE50_25445) 58081..58434 - 354 WP_000423602 hypothetical protein -
OQE50_RS25450 (OQE50_25450) 59579..60160 - 582 Protein_58 RepB family plasmid replication initiator protein -
OQE50_RS25460 (OQE50_25460) 61498..61806 - 309 Protein_60 protein RepA -

Host bacterium

ID   10759 GenBank   NZ_CP110879
Plasmid name   pCTX-M_SCLZS19 Incompatibility group   IncHI2A
Plasmid size   282472 bp Coordinate of oriT [Strand]   140032..140285 [+]
Host baterium   Enterobacter kobei strain SCLZS19

Cargo genes

Drug resistance gene   mcr-9, blaCTX-M-9, sul1, qacE, aadA2, ant(2'')-Ia
Virulence gene   -
Metal resistance gene   terE, terD, terC, terB, terA, terZ, terW
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -