Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 110323 |
| Name | oriT_p7_SCLZS19  |
| Organism | Enterobacter kobei strain SCLZS19 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP110877 (38943..39045 [+], 103 nt) |
| oriT length | 103 nt |
| IRs (inverted repeats) | 64..70, 73..79 (CCACCGT..ACGGTGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 103 nt
>oriT_p7_SCLZS19
AGCCTCGCAGAGCAGGATTCCCGTTGAGCAGCCCAGCTGCGAATAAGGGAAGGTAAAGTTGGACCACCGTTTACGGTGGGCCTACTTTACATATCCTGCCCGG
AGCCTCGCAGAGCAGGATTCCCGTTGAGCAGCCCAGCTGCGAATAAGGGAAGGTAAAGTTGGACCACCGTTTACGGTGGGCCTACTTTACATATCCTGCCCGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 6847 | GenBank | WP_050580356 |
| Name | Relaxase_OQE50_RS24640_p7_SCLZS19 |
UniProt ID | _ |
| Length | 869 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 869 a.a. Molecular weight: 96507.42 Da Isoelectric Point: 10.4435
>WP_050580356.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MKNIKKSSFSGLADYITNPQSKQERVGVIKATNCQSQDVQDAVTEVNITQRLNTRSESDKTYHLVISFRP
GEKPSEDVLNAIEESICQSLGFGDHQRLSAVHHDTDNLHIHVAINKINPDTLTIHNPYNDYRVLAESCAY
LEKKYGLEVDNHMPTRSASENRAKDMERHSGIQSLLSWVKAECAEQLLNANSWQELHQAMRQNGLELRQK
GNGFVIAGPDGLAVKASSVDRSLSRAALEKKLGSFESSIPDTKPSRPSPEAIKQRFGSAANVKRPGDRPP
PMRENRQVHLGDVPVMQINSGKRYESKPLGSQGANTTELYGRYQSEQQRLTAARNEALRLAKVKRDRLIE
NAKRSGRLKRATIKLLKGPGVNKRYLYGLASKALGEELERANAIYKKDRQAAYDIHKRRTWADWLQFKAA
GGDDEALKTLRQRNAQQARKEANILHGDKVKKDGRIPGLKPDSVTKEGTIIYRVGESAIRDGGKRLDVSR
DAGDDGLHAALLMAKHRYGDKLTVNGSFEFKKRIVAVAAARELDVSFADPELEKLRQILTKKAAREREYI
HIPFEKRGKARQAGAMWDTEKKSWFVGPYATRDRIENFIRMNEEKDNEQRRQRYAGTERTDAGRERSGNG
NATGANEPGVTGRSTSADAGNGYGTSSSRRRTGTGDAVTTAGSQPGRSDDTRTGRSRAAEPNGERGHSKP
NVARPGRQPPPTAKNRLRAMSELGMVRIAGGSEVLLQGNVSDQLEHKTAERADQLRRDMAGAGGITTPAE
VKKPLSSAEKYINEREQKRLKGFDIPKHRGYNSTDKGLFEYAGQRKSGLETLALLKRDDEIVVMPVSDYT
ARRLSNLKVGESVTLTPSGSVRTTKGRSR
MKNIKKSSFSGLADYITNPQSKQERVGVIKATNCQSQDVQDAVTEVNITQRLNTRSESDKTYHLVISFRP
GEKPSEDVLNAIEESICQSLGFGDHQRLSAVHHDTDNLHIHVAINKINPDTLTIHNPYNDYRVLAESCAY
LEKKYGLEVDNHMPTRSASENRAKDMERHSGIQSLLSWVKAECAEQLLNANSWQELHQAMRQNGLELRQK
GNGFVIAGPDGLAVKASSVDRSLSRAALEKKLGSFESSIPDTKPSRPSPEAIKQRFGSAANVKRPGDRPP
PMRENRQVHLGDVPVMQINSGKRYESKPLGSQGANTTELYGRYQSEQQRLTAARNEALRLAKVKRDRLIE
NAKRSGRLKRATIKLLKGPGVNKRYLYGLASKALGEELERANAIYKKDRQAAYDIHKRRTWADWLQFKAA
GGDDEALKTLRQRNAQQARKEANILHGDKVKKDGRIPGLKPDSVTKEGTIIYRVGESAIRDGGKRLDVSR
DAGDDGLHAALLMAKHRYGDKLTVNGSFEFKKRIVAVAAARELDVSFADPELEKLRQILTKKAAREREYI
HIPFEKRGKARQAGAMWDTEKKSWFVGPYATRDRIENFIRMNEEKDNEQRRQRYAGTERTDAGRERSGNG
NATGANEPGVTGRSTSADAGNGYGTSSSRRRTGTGDAVTTAGSQPGRSDDTRTGRSRAAEPNGERGHSKP
NVARPGRQPPPTAKNRLRAMSELGMVRIAGGSEVLLQGNVSDQLEHKTAERADQLRRDMAGAGGITTPAE
VKKPLSSAEKYINEREQKRLKGFDIPKHRGYNSTDKGLFEYAGQRKSGLETLALLKRDDEIVVMPVSDYT
ARRLSNLKVGESVTLTPSGSVRTTKGRSR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 7653 | GenBank | WP_024252940 |
| Name | t4cp2_OQE50_RS24630_p7_SCLZS19 |
UniProt ID | _ |
| Length | 648 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 648 a.a. Molecular weight: 71684.76 Da Isoelectric Point: 8.1395
>WP_024252940.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MRKPNNAVGPQVRNKKKTARGKLIPAMAITSLAAGIQAATQYFAYSFNYQELLGPNFNHIYAPWSYFKWY
SAWHDRLPDAFFAAGSVGATVAAGGLVLTALTSMMKANSSKANEYLHGSARWADEQDIKAAGLLGNDEGV
YVGAWEDKNGQLQYLRHNGPEHVLTYAPTRSGKGVGLVVPTLLSWKHSTVVADLKGELWAMTAGWRKEYA
KNLCLKFEPAAANGSVAWNPLDEIRVGTENEVGDVQNLATLIVDPDGKGLNDHWQKTSQALLVGVILHVL
YKHKNDGTPATLPYVDSIMADPERGAGELWMEMTQYGHVNGENHPVVGAAGRDMMDRPEEEAGSVLSTAK
SYLALFRDPVVARNVSESHFKIKDLMNHDDPVSLYIVTQPNDKARLRPLVRIMLNMIVRLLADKMEFERV
NNNLTLWQRFKQAFGFSVQGTKRVQTKKTYKHRLLAMIDEFPSLGKLEIIQESLAFLAGYGIKFYLICQD
INQLRSRETGYGPDETITSNCHVQNAYPPNRTETAEHLSKLTGQTTVIKEQITTSGKRASAILGGVSKTM
QEVQRPLLTVDECLRMPGPTKNAEGLITERGDMVVYVAGFPAIYGKQPLFFQDEIFSLRASVPEPKTTDR
IRAAVAANDDASNEAIAI
MRKPNNAVGPQVRNKKKTARGKLIPAMAITSLAAGIQAATQYFAYSFNYQELLGPNFNHIYAPWSYFKWY
SAWHDRLPDAFFAAGSVGATVAAGGLVLTALTSMMKANSSKANEYLHGSARWADEQDIKAAGLLGNDEGV
YVGAWEDKNGQLQYLRHNGPEHVLTYAPTRSGKGVGLVVPTLLSWKHSTVVADLKGELWAMTAGWRKEYA
KNLCLKFEPAAANGSVAWNPLDEIRVGTENEVGDVQNLATLIVDPDGKGLNDHWQKTSQALLVGVILHVL
YKHKNDGTPATLPYVDSIMADPERGAGELWMEMTQYGHVNGENHPVVGAAGRDMMDRPEEEAGSVLSTAK
SYLALFRDPVVARNVSESHFKIKDLMNHDDPVSLYIVTQPNDKARLRPLVRIMLNMIVRLLADKMEFERV
NNNLTLWQRFKQAFGFSVQGTKRVQTKKTYKHRLLAMIDEFPSLGKLEIIQESLAFLAGYGIKFYLICQD
INQLRSRETGYGPDETITSNCHVQNAYPPNRTETAEHLSKLTGQTTVIKEQITTSGKRASAILGGVSKTM
QEVQRPLLTVDECLRMPGPTKNAEGLITERGDMVVYVAGFPAIYGKQPLFFQDEIFSLRASVPEPKTTDR
IRAAVAANDDASNEAIAI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 5643..15886
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| OQE50_RS24410 (OQE50_24410) | 1396..1671 | - | 276 | WP_266074283 | hypothetical protein | - |
| OQE50_RS24415 (OQE50_24415) | 1672..1919 | - | 248 | Protein_3 | antirestriction protein ArdR | - |
| OQE50_RS24420 (OQE50_24420) | 1919..2227 | - | 309 | WP_003019666 | HigA family addiction module antitoxin | - |
| OQE50_RS24425 (OQE50_24425) | 2236..2535 | - | 300 | WP_024252918 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| OQE50_RS24430 (OQE50_24430) | 3548..4426 | - | 879 | WP_032194784 | plasmid replication initiator TrfA | - |
| OQE50_RS24435 (OQE50_24435) | 4507..4878 | - | 372 | WP_004196205 | single-stranded DNA-binding protein | - |
| OQE50_RS24440 (OQE50_24440) | 4984..5388 | + | 405 | WP_003019654 | transcriptional regulator | - |
| OQE50_RS24445 (OQE50_24445) | 5643..6530 | + | 888 | WP_003019652 | P-type conjugative transfer ATPase TrbB | virB11 |
| OQE50_RS24450 (OQE50_24450) | 6644..7027 | + | 384 | WP_003019650 | TrbC/VirB2 family protein | virB2 |
| OQE50_RS24455 (OQE50_24455) | 7030..7356 | + | 327 | WP_003019648 | conjugal transfer protein TrbD | virB3 |
| OQE50_RS24460 (OQE50_24460) | 7353..9896 | + | 2544 | WP_065809913 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| OQE50_RS24465 (OQE50_24465) | 9893..10600 | + | 708 | WP_032310198 | VirB8/TrbF family protein | virB8 |
| OQE50_RS24470 (OQE50_24470) | 10616..11506 | + | 891 | WP_024252960 | P-type conjugative transfer protein TrbG | virB9 |
| OQE50_RS24475 (OQE50_24475) | 11521..11961 | + | 441 | WP_024252959 | conjugal transfer protein TrbH | - |
| OQE50_RS24480 (OQE50_24480) | 11965..13359 | + | 1395 | WP_024252958 | TrbI/VirB10 family protein | virB10 |
| OQE50_RS24485 (OQE50_24485) | 13372..14142 | + | 771 | WP_004196177 | P-type conjugative transfer protein TrbJ | virB5 |
| OQE50_RS24490 (OQE50_24490) | 14155..14328 | + | 174 | WP_003019629 | entry exclusion lipoprotein TrbK | - |
| OQE50_RS24495 (OQE50_24495) | 14333..15886 | + | 1554 | WP_050563829 | P-type conjugative transfer protein TrbL | virB6 |
| OQE50_RS24500 (OQE50_24500) | 15897..16202 | + | 306 | WP_003019624 | IncN plasmid KikA protein | - |
| OQE50_RS24505 (OQE50_24505) | 16218..16988 | + | 771 | WP_065809914 | conjugal transfer protein | - |
| OQE50_RS24510 (OQE50_24510) | 17005..17691 | + | 687 | WP_074422775 | TraX family protein | - |
| OQE50_RS27710 | 18136..18231 | + | 96 | WP_003019619 | DinQ-like type I toxin DqlB | - |
| OQE50_RS24515 (OQE50_24515) | 18337..18708 | - | 372 | WP_021520164 | helix-turn-helix transcriptional regulator | - |
| OQE50_RS24520 (OQE50_24520) | 19126..19446 | - | 321 | WP_021520162 | hypothetical protein | - |
| OQE50_RS24525 (OQE50_24525) | 19446..19694 | - | 249 | WP_021520161 | hypothetical protein | - |
| OQE50_RS24530 (OQE50_24530) | 19879..20382 | - | 504 | WP_039696286 | thermonuclease family protein | - |
| OQE50_RS24535 (OQE50_24535) | 20382..20633 | - | 252 | WP_052319488 | hypothetical protein | - |
Host bacterium
| ID | 10758 | GenBank | NZ_CP110877 |
| Plasmid name | p7_SCLZS19 | Incompatibility group | IncP1 |
| Plasmid size | 52089 bp | Coordinate of oriT [Strand] | 38943..39045 [+] |
| Host baterium | Enterobacter kobei strain SCLZS19 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |