Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 110111 |
| Name | oriT_pQZ076-1  |
| Organism | Enterococcus faecalis strain QZ076 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP098026 (24453..24502 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | 34..35 |
| Conserved sequence flanking the nic site |
GCTTGCAAAA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pQZ076-1
AATATCGCAACATGCTAGCATGTTGCTCCGCTTGCAAAAAGAAAGCCTAC
AATATCGCAACATGCTAGCATGTTGCTCCGCTTGCAAAAAGAAAGCCTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 6675 | GenBank | WP_029888123 |
| Name | Relaxase_NAG05_RS14830_pQZ076-1 |
UniProt ID | _ |
| Length | 561 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 561 a.a. Molecular weight: 65380.95 Da Isoelectric Point: 6.0136
>WP_029888123.1 relaxase/mobilization nuclease domain-containing protein [Enterococcus faecalis]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKRVFEQISDKIAAKEGAKIIEKSPKQT
HTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYG
YGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVM
QTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSNLLLENELQGDPEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQTLLESKLERTRNEINQLHEIQAVQEKEPEKQTDIKPK
L
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKRVFEQISDKIAAKEGAKIIEKSPKQT
HTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYG
YGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVM
QTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSNLLLENELQGDPEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQTLLESKLERTRNEINQLHEIQAVQEKEPEKQTDIKPK
L
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 3606 | GenBank | WP_002393737 |
| Name | WP_002393737_pQZ076-1 |
UniProt ID | A0A8E7CBI6 |
| Length | 118 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 118 a.a. Molecular weight: 13965.01 Da Isoelectric Point: 9.6545
>WP_002393737.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Enterococcus]
MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKDQS
MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKDQS
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 7387 | GenBank | WP_010717222 |
| Name | t4cp2_NAG05_RS14810_pQZ076-1 |
UniProt ID | _ |
| Length | 609 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 609 a.a. Molecular weight: 70128.19 Da Isoelectric Point: 6.1807
>WP_010717222.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Enterococcus]
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYPKGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYPKGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 2273..21808
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| NAG05_RS14705 (NAG05_14705) | 1648..1983 | + | 336 | WP_128701276 | DUF5406 domain-containing protein | - |
| NAG05_RS14710 (NAG05_14710) | 2273..6190 | + | 3918 | WP_250652959 | LPXTG-anchored aggregation substance | prgB |
| NAG05_RS14715 (NAG05_14715) | 6530..6670 | + | 141 | WP_113826368 | LPXTG cell wall anchor domain-containing protein | prgC |
| NAG05_RS14720 (NAG05_14720) | 6733..7089 | + | 357 | WP_010711024 | pheromone response system RNA-binding regulator PrgU | - |
| NAG05_RS14725 (NAG05_14725) | 7117..7974 | + | 858 | WP_002367673 | pheromone response LPXTG-anchored adhesin PrgC | prgC |
| NAG05_RS14730 (NAG05_14730) | 8016..8915 | + | 900 | WP_128701257 | type III secretion system protein PrgD | - |
| NAG05_RS14735 (NAG05_14735) | 8935..9369 | + | 435 | WP_002365857 | hypothetical protein | - |
| NAG05_RS14740 (NAG05_14740) | 9416..9643 | + | 228 | WP_002365858 | hypothetical protein | - |
| NAG05_RS14745 (NAG05_14745) | 9656..9952 | + | 297 | WP_128701256 | type III secretion system protein PrgG | - |
| NAG05_RS14750 (NAG05_14750) | 9967..10767 | + | 801 | WP_010774271 | type IV secretion system protein | prgHb |
| NAG05_RS14755 (NAG05_14755) | 10769..11122 | + | 354 | WP_002367919 | PrgI family mobile element protein | prgIc |
| NAG05_RS14760 (NAG05_14760) | 11076..13466 | + | 2391 | WP_016632782 | VirB4-like conjugal transfer ATPase, CD1110 family | virb4 |
| NAG05_RS14765 (NAG05_14765) | 13478..16093 | + | 2616 | WP_250652960 | phage tail tip lysozyme | prgK |
| NAG05_RS14770 (NAG05_14770) | 16117..16743 | + | 627 | WP_128701254 | hypothetical protein | prgL |
| NAG05_RS14775 (NAG05_14775) | 16730..16975 | + | 246 | WP_002410289 | hypothetical protein | - |
| NAG05_RS14780 (NAG05_14780) | 16959..17567 | + | 609 | WP_128701253 | PcfA protein | - |
| NAG05_RS14785 (NAG05_14785) | 17587..17922 | + | 336 | WP_128701252 | hypothetical protein | - |
| NAG05_RS14790 (NAG05_14790) | 17992..18747 | + | 756 | WP_016616521 | Fic family protein | - |
| NAG05_RS14795 (NAG05_14795) | 18764..19222 | + | 459 | WP_116593455 | single-stranded DNA-binding protein | - |
| NAG05_RS14800 (NAG05_14800) | 19268..19465 | + | 198 | WP_002425087 | hypothetical protein | - |
| NAG05_RS14805 (NAG05_14805) | 19494..19979 | + | 486 | WP_002386493 | PcfB family protein | gbs1365 |
| NAG05_RS14810 (NAG05_14810) | 19979..21808 | + | 1830 | WP_010717222 | VirD4-like conjugal transfer protein, CD1115 family | virb4 |
| NAG05_RS14815 (NAG05_14815) | 21859..24018 | + | 2160 | WP_250652961 | ArdC-like ssDNA-binding domain-containing protein | - |
| NAG05_RS14820 (NAG05_14820) | 24051..24323 | + | 273 | WP_002362409 | LtrD | - |
| NAG05_RS14825 (NAG05_14825) | 24569..24925 | + | 357 | WP_002393737 | plasmid mobilization relaxosome protein MobC | - |
| NAG05_RS14830 (NAG05_14830) | 24926..26611 | + | 1686 | WP_029888123 | relaxase/mobilization nuclease domain-containing protein | - |
Host bacterium
| ID | 10546 | GenBank | NZ_CP098026 |
| Plasmid name | pQZ076-1 | Incompatibility group | - |
| Plasmid size | 65961 bp | Coordinate of oriT [Strand] | 24453..24502 [+] |
| Host baterium | Enterococcus faecalis strain QZ076 |
Cargo genes
| Drug resistance gene | cfr, fexA |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |