Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   110080
Name   oriT1_p1762RFN in_silico
Organism   Enterobacter hormaechei subsp. oharae strain NMI1762/19
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MZ779047 (24288..24382 [+], 95 nt)
oriT length   95 nt
IRs (inverted repeats)      73..78, 85..90  (AAAAAA..TTTTTT)
 73..78, 84..89  (AAAAAA..TTTTTT)
 27..34, 37..44  (AGCGTGAT..ATCACGCT)
 13..19, 31..37  (TAAATCA..TGATTTA)
Location of nic site      55..56
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 95 nt

>oriT1_p1762RFN
TTTTTTTTCTTTTAAATCAGTGAGATAGCGTGATTTATCACGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   6657 GenBank   WP_012561166
Name   mobF_M2543_RS00300_p1762RFN insolico UniProt ID   D7RU03
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120141.30 Da        Isoelectric Point: 6.5229

>WP_012561166.1 MULTISPECIES: MobF family relaxase [Gammaproteobacteria]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure


Source ID Structure
AlphaFold DB D7RU03


Auxiliary protein

ID   3589 GenBank   WP_001749975
Name   WP_001749975_p1762RFN insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   7363 GenBank   WP_000342688
Name   t4cp2_M2543_RS00305_p1762RFN insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 82314..92639

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2543_RS00450 78159..79592 - 1434 WP_001288432 DNA cytosine methyltransferase -
M2543_RS00455 79691..79963 + 273 Protein_90 IS1 family transposase -
M2543_RS00460 79974..80180 - 207 WP_001749967 hypothetical protein -
M2543_RS00465 80185..80832 - 648 WP_015344958 restriction endonuclease -
M2543_RS00470 80882..81115 - 234 WP_001191790 hypothetical protein -
M2543_RS00475 81229..81543 - 315 WP_001749965 TrbM/KikA/MpfK family conjugal transfer protein -
M2543_RS00480 81540..81884 - 345 WP_001749964 hypothetical protein -
M2543_RS00485 81900..82271 - 372 WP_011867773 H-NS family nucleoid-associated regulatory protein -
M2543_RS00490 82314..83048 + 735 WP_001749963 lytic transglycosylase domain-containing protein virB1
M2543_RS00495 83057..83338 + 282 WP_000440698 transcriptional repressor KorA -
M2543_RS00500 83348..83641 + 294 WP_001749962 hypothetical protein virB2
M2543_RS00505 83691..84008 + 318 WP_000496058 VirB3 family type IV secretion system protein virB3
M2543_RS00510 84008..86608 + 2601 WP_001749961 VirB4 family type IV secretion/conjugal transfer ATPase virb4
M2543_RS00515 86626..87339 + 714 WP_001749960 type IV secretion system protein virB5
M2543_RS00520 87347..87574 + 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
M2543_RS00525 87590..88630 + 1041 WP_001749958 type IV secretion system protein virB6
M2543_RS00770 88731..88859 + 129 WP_071882546 conjugal transfer protein TraN -
M2543_RS00530 88849..89547 + 699 WP_000646594 type IV secretion system protein virB8
M2543_RS00535 89558..90442 + 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
M2543_RS00540 90442..91602 + 1161 WP_000101710 type IV secretion system protein VirB10 virB10
M2543_RS00545 91644..92639 + 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
M2543_RS00550 92639..93172 + 534 WP_000792636 phospholipase D family protein -
M2543_RS00775 93346..93633 - 288 Protein_111 DUF6710 family protein -
M2543_RS00560 93900..94079 + 180 Protein_112 helix-turn-helix domain-containing protein -
M2543_RS00760 94045..94167 - 123 WP_001437553 hypothetical protein -
M2543_RS00565 94144..94848 + 705 WP_001067855 IS6-like element IS26 family transposase -
M2543_RS00570 94987..95169 - 183 Protein_115 DUF4158 domain-containing protein -
M2543_RS00575 95331..95945 + 615 WP_014839983 recombinase family protein -
M2543_RS00580 96264..96920 - 657 WP_001516695 quinolone resistance pentapeptide repeat protein QnrS1 -

Host bacterium

ID   10515 GenBank   NZ_MZ779047
Plasmid name   p1762RFN Incompatibility group   IncR
Plasmid size   124524 bp Coordinate of oriT [Strand]   24288..24382 [+]; 56806..56906 [+]
Host baterium   Enterobacter hormaechei subsp. oharae strain NMI1762/19

Cargo genes

Drug resistance gene   dfrA14, blaNDM-1, qnrS1, aac(6')-Ib
Virulence gene   -
Metal resistance gene   merR, merT, merP, merC, merA, merD, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -