Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 110079 |
| Name | oriT_p1997F  |
| Organism | Enterobacter hormaechei subsp. steigerwaltii strain NMI1997_18 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MZ750393 (71843..71924 [+], 82 nt) |
| oriT length | 82 nt |
| IRs (inverted repeats) | 27..32, 35..40 (GCCACC..GGTGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 82 nt
>oriT_p1997F
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 6656 | GenBank | WP_015632446 |
| Name | Relaxase_LN387_RS00425_p1997F |
UniProt ID | R4WBX2 |
| Length | 642 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 642 a.a. Molecular weight: 74631.63 Da Isoelectric Point: 9.3060
>WP_015632446.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDQRFRRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVTCQHNLMSVETAAMEMNSIAMQNLHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNLVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNTMFREKNASWEKLHQVMNDAGLML
EPSGKGLVVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSSRSAEMVKQSVQTPYSDRLHVRDRGA
RAERRQARAEARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIELKAERRKLYDEGRMRPLTYRAWTEQQALHGDQAALSQLRGWAYRQNRKNRTPQASDSI
IFCAPADDTSLIKADGYDARLYRDGAVVYSRHGQDAIIDRGDRVEVINPYDNGDLNTRIAVDMVAWKSGE
RIEFSRDGSFAYAAGDAVAAHNLHHRDSILVPSNREQYDYTREKFNQLKGRYTDREREAVRQQYDNRYYD
DVRHNDDNSYKP
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDQRFRRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVTCQHNLMSVETAAMEMNSIAMQNLHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNLVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNTMFREKNASWEKLHQVMNDAGLML
EPSGKGLVVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSSRSAEMVKQSVQTPYSDRLHVRDRGA
RAERRQARAEARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIELKAERRKLYDEGRMRPLTYRAWTEQQALHGDQAALSQLRGWAYRQNRKNRTPQASDSI
IFCAPADDTSLIKADGYDARLYRDGAVVYSRHGQDAIIDRGDRVEVINPYDNGDLNTRIAVDMVAWKSGE
RIEFSRDGSFAYAAGDAVAAHNLHHRDSILVPSNREQYDYTREKFNQLKGRYTDREREAVRQQYDNRYYD
DVRHNDDNSYKP
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | R4WBX2 |
T4CP
| ID | 7362 | GenBank | WP_015632442 |
| Name | trbC_LN387_RS00460_p1997F |
UniProt ID | _ |
| Length | 730 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 730 a.a. Molecular weight: 82688.74 Da Isoelectric Point: 5.8919
>WP_015632442.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MQQKQKPVDAARLQRQVHQGMVEEMAYRTGPLLLAIIVIFGAAIVWPVLLWVAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLSRHMDNAAGVFYTGYERMLDGGREIWLRMDDLTRHLI
LLATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFIWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTITDPTETAEFAAKSAGMEYQAIQDSMEYRPGAISGSYADTGRVTVQEKSKLPFDELQTLN
QGEQATIFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLAHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPDAVQRATQLLSLAASVLTETRGRYSVEYPEP
EKLSVKKDEFEKYQHIHQSAVTQQADGFVY
MQQKQKPVDAARLQRQVHQGMVEEMAYRTGPLLLAIIVIFGAAIVWPVLLWVAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLSRHMDNAAGVFYTGYERMLDGGREIWLRMDDLTRHLI
LLATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFIWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTITDPTETAEFAAKSAGMEYQAIQDSMEYRPGAISGSYADTGRVTVQEKSKLPFDELQTLN
QGEQATIFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLAHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPDAVQRATQLLSLAASVLTETRGRYSVEYPEP
EKLSVKKDEFEKYQHIHQSAVTQQADGFVY
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 79785..101626
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LN387_RS00440 | 74890..75252 | + | 363 | WP_032495750 | transposase | - |
| LN387_RS00445 | 75249..75598 | + | 350 | Protein_88 | IS66 family insertion sequence element accessory protein TnpB | - |
| LN387_RS00450 | 75628..77217 | + | 1590 | WP_015632444 | IS66 family transposase | - |
| LN387_RS00455 | 77240..77545 | - | 306 | WP_015632443 | phospholipase D-like domain-containing protein | - |
| LN387_RS00460 | 77591..79783 | - | 2193 | WP_015632442 | F-type conjugative transfer protein TrbC | - |
| LN387_RS00465 | 79785..80939 | - | 1155 | WP_015632441 | thioredoxin fold domain-containing protein | trbB |
| LN387_RS00470 | 80887..82305 | - | 1419 | WP_015632440 | hypothetical protein | trbA |
| LN387_RS00475 | 82407..83030 | - | 624 | WP_015632439 | plasmid IncI1-type surface exclusion protein ExcA | - |
| LN387_RS00480 | 83096..85240 | - | 2145 | WP_015632438 | DotA/TraY family protein | traY |
| LN387_RS00485 | 85282..85863 | - | 582 | WP_015632437 | conjugal transfer protein TraX | - |
| LN387_RS00490 | 85860..87020 | - | 1161 | WP_032495747 | conjugal transfer protein TraW | traW |
| LN387_RS00495 | 87067..90114 | - | 3048 | WP_015632435 | conjugal transfer protein | traU |
| LN387_RS00500 | 90124..90687 | - | 564 | WP_032495746 | hypothetical protein | traT |
| LN387_RS00505 | 90731..91132 | - | 402 | WP_015632433 | DUF6750 family protein | traR |
| LN387_RS00510 | 91186..91719 | - | 534 | WP_015632432 | conjugal transfer protein TraQ | traQ |
| LN387_RS00515 | 91729..92448 | - | 720 | WP_015632431 | conjugal transfer protein TraP | traP |
| LN387_RS00520 | 92454..93794 | - | 1341 | WP_015632430 | conjugal transfer protein TraO | traO |
| LN387_RS00525 | 93798..94901 | - | 1104 | WP_015632429 | DotH/IcmK family type IV secretion protein | traN |
| LN387_RS00530 | 94912..95616 | - | 705 | WP_015632428 | DotI/IcmL/TraM family protein | traM |
| LN387_RS00535 | 95652..96005 | - | 354 | WP_015632427 | hypothetical protein | traL |
| LN387_RS00540 | 96039..99362 | - | 3324 | WP_032495745 | LPD7 domain-containing protein | - |
| LN387_RS00545 | 99380..99646 | - | 267 | WP_032441926 | IcmT/TraK family protein | traK |
| LN387_RS00550 | 99643..100830 | - | 1188 | WP_032495744 | plasmid transfer ATPase TraJ | virB11 |
| LN387_RS00555 | 100808..101626 | - | 819 | WP_015632425 | type IV secretory system conjugative DNA transfer family protein | traI |
| LN387_RS00560 | 101623..102090 | - | 468 | WP_015632424 | DotD/TraH family lipoprotein | - |
| LN387_RS00565 | 102113..102592 | - | 480 | WP_015632423 | lytic transglycosylase domain-containing protein | - |
| LN387_RS00570 | 103290..103601 | - | 312 | WP_015632421 | hypothetical protein | - |
| LN387_RS00575 | 103635..103838 | - | 204 | WP_032495743 | hypothetical protein | - |
| LN387_RS00580 | 103856..104323 | - | 468 | WP_032495742 | transcription termination/antitermination NusG family protein | - |
| LN387_RS00585 | 104657..105235 | + | 579 | WP_015632419 | hypothetical protein | - |
| LN387_RS00590 | 105202..105687 | + | 486 | WP_015632418 | hypothetical protein | - |
| LN387_RS00595 | 105687..106073 | + | 387 | WP_015632417 | acyl-CoA thioesterase | - |
Host bacterium
| ID | 10514 | GenBank | NZ_MZ750393 |
| Plasmid name | p1997F | Incompatibility group | IncFII |
| Plasmid size | 106552 bp | Coordinate of oriT [Strand] | 71843..71924 [+] |
| Host baterium | Enterobacter hormaechei subsp. steigerwaltii strain NMI1997_18 |
Cargo genes
| Drug resistance gene | blaNDM-1, aac(6')-Ib-cr, blaOXA-1, aac(3)-IIa, blaCTX-M-3, aac(6')-Ib |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |