Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   110070
Name   oriT_N2-2|unnamed1 in_silico
Organism   Bacillus subtilis subsp. subtilis strain N2-2
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP032864 (15425..15786 [+], 362 nt)
oriT length   362 nt
IRs (inverted repeats)      188..193, 198..203  (CACCAT..ATGGTG)
 171..178, 180..187  (AAACCGCA..TGCGGTTT)
 116..122, 130..136  (ACATAAA..TTTATGT)
 84..89, 101..106  (ATTTAG..CTAAAT)
 84..89, 92..97  (ATTTAG..CTAAAT)
 62..67, 75..80  (CATAAA..TTTATG)
 48..54, 65..71  (TTATTTT..AAAATAA)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 362 nt

>oriT_N2-2|unnamed1
AAAGAGCAATCTCGTCATCGAAGACTAAATTTCTGTATGGAAAACAGTTATTTTTCGTGTGCATAAAATAAAGATTTATGTGCATTTAGTTCTAAATCACCTAAATAATGGTTGAACATAAATGTTTTTTTTATGTGCATTCAGCAATAAATCTGGTACCACGAAAAAACAAACCGCACTGCGGTTTCACCATGCAAATGGTGCCAGTTTCCCCTTATGCTCTTTCCTTTTCCACCCCCTCCTTCCTTCGGAATCGGGGGCCGGCTTTTTGCTGCCGCAAAAAGCCTTGGAAAAAGAAACACTTATTTGAACAGATCGTTGCAAAGTAATGTGCAGAAATCGATGAAAAGGAGCGTTAACAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   6649 GenBank   WP_013603221
Name   mobP2_D9C17_RS22255_N2-2|unnamed1 insolico UniProt ID   E9RJ23
Length   410 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 48596.08 Da        Isoelectric Point: 10.0206

>WP_013603221.1 MULTISPECIES: MobP2 family relaxase [Bacillaceae]
MDSPGVVLVSKYVSGKSTKFSKYVNYINRDEAVRTEKFQTYNVNKLDGYNQYMGNPEKSSGIFTQHKDSL
SPVEKNQLKEIFRQAQKNDSVMWQDVISFDNKWLEERGIYNSQTGWVNEGAIQNSIRKGMEVLLREEQLE
QSGVWSAAIHYNTDNIHVHIALVEPNPTKEYGVFTNKKTGEVYQARRGNRKLKTLDKMKSKVANTLMDRD
KELSKISQLIHDRIAPKGLKFQPRLDTYMTKMYNHIYENLPEDMRLWKYNNNALNNIRPEIDSVITMYIQ
KYHPEDYKELDQSLKEEMEFRKSVYGDGPKQVERYKEYRKNKHKELYTKLGNSMLKEMAEIRRRESQSKQ
MNRSGTYAPSSVSNRQWDTKGRRIKRSDINKIKHALDKDYQSMKNMRKYQQMQYEMEQSR

  Protein domains


Predicted by InterproScan.

(3-389)


  Protein structure


Source ID Structure
AlphaFold DB E9RJ23


T4CP


ID   7356 GenBank   WP_013603210
Name   t4cp2_D9C17_RS22205_N2-2|unnamed1 insolico UniProt ID   _
Length   786 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 786 a.a.        Molecular weight: 90624.17 Da        Isoelectric Point: 4.9504

>WP_013603210.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Bacillus]
MHKEFKQWKKVFADKYFLIAFTLFCYVAVTCITNFFIHLLKQIPVILSSFKNFDQSQLSSPFSSMTWKWF
FEFDWSMGIVYGVVYVLASIFILRQVYRFRIAFRDINKQTKGTARWTEIMEIQETYKAVKDDDNEYEGSA
GMPIVHFNDHLYVDTNSTHTLVVASTQSGKTETYSYPYLDVIMRAKEKDSVVITDIKGDMLKNTRAEFEK
YGYEVMCFNLLNPYWGMAYNPLELVKQAYLKKDYSKAQMLCNTLSYSLFHNDKSHADPMWENASIALVNA
LILAVCDLCIKNGQPENITMYTLTVMLNELGSNPDEDGYTRLDHFFGNLPPSHPAKLQYGTIQFSQGITR
SGIYTGTMAKLKNYTYDTIARMTARNDLNIEDLAYGEKPVALFIVYPDWDDSNYTIISTFLSQVNAVLSE
KATLSKESTLPRKVRFLFEEVANIPPIEGLNRSLAVGLSRGMLYTLVIQNVSQLRDVYGDDMAVAIMGNL
GNQIYIMSDEWEDAEKFSEKLGVTTVISADRQGDLMDVHKSYSEREEERPLMLPDELRRLKKGEWVVLRT
KKREDLKRNRVVPYPIFASLDNGTNMLHRYEYLMHRFDNKIALGDLGFRGEHETLDLESLLIEFEFNEPV
KEERKSKPGKGKKQKAKKAPGEPVSSEFPLPEEPIGFTESSEGMDSLFASIPVENMNEHGFYEDPPMNES
LHEDDLEASEEDIIMNSLEEVYETSTPISDAIKPDHYVYIKLLAHKQLSESEYAYFDSLKTVEELRAFFR
APEKKEIYEQIKTHIE

  Protein domains


Predicted by InterproScan.

(148-567)

  Protein structure



No available structure.




Host bacterium


ID   10505 GenBank   NZ_CP032864
Plasmid name   N2-2|unnamed1 Incompatibility group   -
Plasmid size   64616 bp Coordinate of oriT [Strand]   15425..15786 [+]
Host baterium   Bacillus subtilis subsp. subtilis strain N2-2

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -