Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 110000 |
| Name | oriT_p2017-45-51-04-01_4  |
| Organism | Enterobacter roggenkampii strain 2017-45-51-04-01 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP109729 (59873..59977 [-], 105 nt) |
| oriT length | 105 nt |
| IRs (inverted repeats) | 32..37, 40..45 (GCCGGC..GCCGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 105 nt
>oriT_p2017-45-51-04-01_4
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 6602 | GenBank | WP_004206924 |
| Name | Relaxase_OI980_RS25175_p2017-45-51-04-01_4 |
UniProt ID | A0A2V4FJ40 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75087.84 Da Isoelectric Point: 10.0285
>WP_004206924.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2V4FJ40 |
T4CP
| ID | 7292 | GenBank | WP_004206885 |
| Name | t4cp2_OI980_RS25040_p2017-45-51-04-01_4 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 26157..57075
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| OI980_RS24985 (OI980_24980) | 21525..21743 | + | 219 | WP_004187411 | hypothetical protein | - |
| OI980_RS24990 (OI980_24985) | 21746..21955 | + | 210 | WP_004187413 | hypothetical protein | - |
| OI980_RS24995 (OI980_24990) | 22078..23343 | - | 1266 | WP_024190314 | translesion error-prone DNA polymerase V subunit UmuC | - |
| OI980_RS25000 (OI980_24995) | 23331..23765 | - | 435 | WP_044068898 | translesion error-prone DNA polymerase V autoproteolytic subunit | - |
| OI980_RS25005 (OI980_25000) | 23858..24190 | - | 333 | WP_004187427 | type II toxin-antitoxin system toxin endoribonuclease PemK-mt | - |
| OI980_RS25010 (OI980_25005) | 24192..24449 | - | 258 | WP_004187429 | type II toxin-antitoxin system antitoxin PemI | - |
| OI980_RS25015 (OI980_25010) | 24541..25194 | - | 654 | WP_004206890 | type II CAAX endopeptidase family protein | - |
| OI980_RS25020 (OI980_25015) | 25274..25657 | + | 384 | WP_004206889 | DUF1496 domain-containing protein | - |
| OI980_RS25025 (OI980_25020) | 25762..26157 | + | 396 | WP_004187436 | lytic transglycosylase domain-containing protein | - |
| OI980_RS25030 (OI980_25025) | 26157..27464 | + | 1308 | WP_015059988 | hypothetical protein | trbA |
| OI980_RS25035 (OI980_25030) | 27475..28425 | + | 951 | WP_004206886 | DsbC family protein | trbB |
| OI980_RS25040 (OI980_25035) | 28438..30525 | + | 2088 | WP_004206885 | conjugal transfer protein TrbC | - |
| OI980_RS25045 | 30576..30671 | - | 96 | WP_004206884 | DinQ-like type I toxin DqlB | - |
| OI980_RS25050 (OI980_25040) | 31898..32953 | - | 1056 | WP_021526650 | plasmid replication initiator RepA | - |
| OI980_RS25055 (OI980_25045) | 33250..33480 | - | 231 | WP_004187496 | IncL/M type plasmid replication protein RepC | - |
| OI980_RS25060 (OI980_25050) | 33554..34207 | - | 654 | WP_004206935 | hypothetical protein | - |
| OI980_RS25065 (OI980_25055) | 34210..34602 | - | 393 | WP_284608287 | hypothetical protein | traY |
| OI980_RS25070 (OI980_25060) | 34647..35252 | - | 606 | WP_000509966 | recombinase family protein | - |
| OI980_RS25075 (OI980_25065) | 35347..38244 | + | 2898 | WP_284592664 | Tn3-like element Tn5403 family transposase | - |
| OI980_RS25080 (OI980_25070) | 38260..40059 | - | 1800 | WP_284608288 | DotA/TraY family protein | traY |
| OI980_RS25085 (OI980_25075) | 40052..40702 | - | 651 | WP_004187488 | hypothetical protein | - |
| OI980_RS25090 (OI980_25080) | 40699..41907 | - | 1209 | WP_004187486 | conjugal transfer protein TraW | traW |
| OI980_RS25095 (OI980_25085) | 41904..44954 | - | 3051 | WP_011154474 | conjugative transfer protein | traU |
| OI980_RS25100 (OI980_25090) | 44951..45445 | - | 495 | WP_004187480 | hypothetical protein | - |
| OI980_RS25105 (OI980_25095) | 45491..45880 | - | 390 | WP_011154472 | DUF6750 family protein | traR |
| OI980_RS25110 (OI980_25100) | 45897..46427 | - | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| OI980_RS25115 (OI980_25105) | 46451..47155 | - | 705 | WP_004206933 | conjugal transfer protein TraP | traP |
| OI980_RS25120 (OI980_25110) | 47167..48516 | - | 1350 | WP_004206932 | conjugal transfer protein TraO | traO |
| OI980_RS25125 (OI980_25115) | 48528..49679 | - | 1152 | WP_015062843 | DotH/IcmK family type IV secretion protein | traN |
| OI980_RS25130 (OI980_25120) | 49688..50401 | - | 714 | WP_223294958 | DotI/IcmL family type IV secretion protein | traM |
| OI980_RS25135 (OI980_25125) | 50469..50879 | + | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| OI980_RS25140 (OI980_25130) | 50908..51063 | + | 156 | WP_172693602 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| OI980_RS25145 (OI980_25135) | 51064..51576 | - | 513 | WP_011091071 | hypothetical protein | traL |
| OI980_RS25150 (OI980_25140) | 51542..54847 | - | 3306 | WP_219818010 | LPD7 domain-containing protein | - |
| OI980_RS25155 (OI980_25145) | 54872..55132 | - | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| OI980_RS25160 (OI980_25150) | 55122..56285 | - | 1164 | WP_004206926 | plasmid transfer ATPase TraJ | virB11 |
| OI980_RS25165 (OI980_25155) | 56296..57075 | - | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| OI980_RS25170 (OI980_25160) | 57072..57572 | - | 501 | WP_004206925 | DotD/TraH family lipoprotein | - |
| OI980_RS25175 (OI980_25165) | 57586..59565 | - | 1980 | WP_004206924 | TraI/MobA(P) family conjugative relaxase | - |
| OI980_RS25180 (OI980_25170) | 59552..59869 | - | 318 | WP_004206923 | plasmid mobilization protein MobA | - |
| OI980_RS25185 (OI980_25175) | 60145..60510 | + | 366 | WP_004206922 | hypothetical protein | - |
| OI980_RS25190 (OI980_25180) | 61011..61547 | - | 537 | WP_004206920 | hypothetical protein | - |
| OI980_RS25195 (OI980_25185) | 61680..61991 | - | 312 | WP_004187333 | hypothetical protein | - |
Host bacterium
| ID | 10435 | GenBank | NZ_CP109729 |
| Plasmid name | p2017-45-51-04-01_4 | Incompatibility group | IncL/M |
| Plasmid size | 63890 bp | Coordinate of oriT [Strand] | 59873..59977 [-] |
| Host baterium | Enterobacter roggenkampii strain 2017-45-51-04-01 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |