Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 109996
• Name: oriT_p2017-45-54-05_1
• Organism: Enterobacter asburiae strain 2017-45-54-05
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP109698 (278423..278677 [-], 255 nt)
• oriT length: 255 nt
• IRs (inverted repeats): 153..158, 160..165 (AAAAGT..ACTTTT) 122..130, 135..143 (TTAAGGCTT..AAGCCTTAA) 49..55, 60..66 (GAATTTT..AAAATTC)
• Location of nic site: 78..79
• Conserved sequence flanking the nic site: TTTGGTTAAA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 255 nt

>oriT_p2017-45-54-05_1
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobH_OI909_RS26815_p2017-45-54-05_1

• ID: 6599
• GenBank: WP_012477394
• Name: mobH_OI909_RS26815_p2017-45-54-05_1
• UniProt ID: A0A2Z2E7B6
• Length: 1050 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1050 a.a.
• Molecular weight: 118067.47 Da
• Isoelectric Point: 4.6347

>WP_012477394.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDT
VETAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFSQLSVSRPEVG
SCATSSSVHNEKLLSEPSELPELNREQNADPQGTNERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFASTESDQNGEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

Protein domains

Predicted by InterproScan.

• Putative helicase (TraI_2) (108-304)

Protein structure

• AlphaFold DB: A0A2Z2E7B6

T4CP

traD_OI909_RS26820_p2017-45-54-05_1

• ID: 7288
• GenBank: WP_001284073
• Name: traD_OI909_RS26820_p2017-45-54-05_1
• UniProt ID: _
• Length: 694 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 694 a.a.
• Molecular weight: 78123.91 Da
• Isoelectric Point: 8.0177

>WP_001284073.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (406-609)

• AAA-like domain (AAA_10) (199-249)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 50126..59333

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OI909_RS25630 (OI909_25625)	45236..45952	-	717	WP_000838219	zinc finger-like domain-containing protein	-
OI909_RS25635 (OI909_25630)	45949..46149	-	201	WP_000594930	hypothetical protein	-
OI909_RS25640 (OI909_25635)	46169..47221	-	1053	WP_001065778	hypothetical protein	-
OI909_RS25645 (OI909_25640)	47698..48573	+	876	WP_000594611	RepB family plasmid replication initiator protein	-
OI909_RS25650 (OI909_25645)	49722..50075	+	354	WP_000423602	hypothetical protein	-
OI909_RS25655 (OI909_25650)	50093..50443	+	351	WP_001447770	type IV conjugative transfer system protein TraL	-
OI909_RS25660 (OI909_25655)	50455..51243	+	789	WP_000783153	TraE/TraK family type IV conjugative transfer system protein	traE
OI909_RS25665 (OI909_25660)	51243..52514	+	1272	WP_000592092	type-F conjugative transfer system secretin TraK	traK
OI909_RS25670 (OI909_25665)	52516..52977	+	462	WP_000521242	plasmid transfer protein HtdO	-
OI909_RS25675 (OI909_25670)	52967..54322	+	1356	WP_000351841	IncHI-type conjugal transfer protein TrhB	traB
OI909_RS25680 (OI909_25675)	54330..54812	+	483	WP_000377632	hypothetical protein	-
OI909_RS25685 (OI909_25680)	54931..55683	+	753	WP_182911723	protein-disulfide isomerase HtdT	-
OI909_RS25690 (OI909_25685)	55693..56643	+	951	WP_001022588	IncHI-type conjugal transfer lipoprotein TrhV	traV
OI909_RS25695 (OI909_25690)	56652..59333	+	2682	WP_000387412	TraC family protein	virb4
OI909_RS25700 (OI909_25695)	61882..62043	+	162	WP_001371949	hypothetical protein	-

Host bacterium

• ID: 10431
• GenBank: NZ_CP109698
• Plasmid name: p2017-45-54-05_1
• Incompatibility group: IncHI2
• Plasmid size: 303280 bp
• Coordinate of oriT [Strand]: 278423..278677 [-]
• Host baterium: Enterobacter asburiae strain 2017-45-54-05

Cargo genes

• Drug resistance gene: ant(2'')-Ia, aadA2, qacE, sul1, qnrA1, tet(A), blaSHV-12, formA, mcr-9
• Virulence gene: -
• Metal resistance gene: terW, terZ, terA, terB, terC, terD, terE, merE, merD, merA, merC, merP, merT, merR, pcoS, pcoE, ncrC, rcnR/yohL, arsC, arsB, arsR, arsH
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -