Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 109742 |
| Name | oriT_145|P2  |
| Organism | Klebsiella oxytoca isolate 145 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_OW967376 (45394..45499 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_145|P2
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 6449 | GenBank | WP_004187323 |
| Name | Relaxase_LQ212_RS30000_145|P2 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 7075 | GenBank | WP_004206885 |
| Name | t4cp2_LQ212_RS29680_145|P2 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 48295..67584
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LQ212_RS29980 (AI2623V1_5844) | 43379..43690 | + | 312 | WP_004187333 | hypothetical protein | - |
| LQ212_RS29985 (AI2623V1_5845) | 43823..44359 | + | 537 | WP_004187332 | hypothetical protein | - |
| LQ212_RS29990 (AI2623V1_5847) | 44861..45256 | - | 396 | WP_019725163 | hypothetical protein | - |
| LQ212_RS29995 | 45291..45818 | + | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| LQ212_RS30000 (AI2623V1_5849) | 45805..47784 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| LQ212_RS30005 (AI2623V1_5850) | 47798..48298 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| LQ212_RS30010 (AI2623V1_5851) | 48295..49074 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| LQ212_RS30015 (AI2623V1_5852) | 49085..50248 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| LQ212_RS30020 (AI2623V1_5853) | 50238..50498 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| LQ212_RS30025 (AI2623V1_5854) | 50523..53960 | + | 3438 | WP_015586048 | LPD7 domain-containing protein | - |
| LQ212_RS30030 (AI2623V1_5855) | 53926..54438 | + | 513 | WP_011091071 | hypothetical protein | traL |
| LQ212_RS30035 (AI2623V1_5856) | 54439..54651 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| LQ212_RS30040 (AI2623V1_5857) | 54623..55033 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| LQ212_RS30045 (AI2623V1_5858) | 55101..55814 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| LQ212_RS30050 | 55823..56974 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| LQ212_RS30055 (AI2623V1_5861) | 56986..58335 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| LQ212_RS30060 (AI2623V1_5862) | 58347..59051 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| LQ212_RS30065 (AI2623V1_5863) | 59075..59605 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| LQ212_RS30070 (AI2623V1_5864) | 59622..60011 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| LQ212_RS30075 (AI2623V1_5865) | 60057..60551 | + | 495 | WP_004187480 | hypothetical protein | - |
| LQ212_RS30080 (AI2623V1_5866) | 60548..63598 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| LQ212_RS30085 (AI2623V1_5867) | 63718..64803 | + | 1086 | WP_228719183 | conjugal transfer protein TraW | traW |
| LQ212_RS30090 (AI2623V1_5868) | 64800..65396 | + | 597 | WP_015060003 | hypothetical protein | - |
| LQ212_RS30095 (AI2623V1_5869) | 65389..67584 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| LQ212_RS30100 (AI2623V1_5870) | 67586..68239 | + | 654 | WP_015060005 | hypothetical protein | - |
| LQ212_RS30105 (AI2623V1_5871) | 68320..68550 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 10177 | GenBank | NZ_OW967376 |
| Plasmid name | 145|P2 | Incompatibility group | IncL/M |
| Plasmid size | 68845 bp | Coordinate of oriT [Strand] | 45394..45499 [+] |
| Host baterium | Klebsiella oxytoca isolate 145 |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |